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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHG181
Library VH
(Link to library)
Clone ID VHG181
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16204-1
Original site URL
Representative seq. ID VHG181P
(Link to Original site)
Representative DNA sequence 
>VHG181 (VHG181Q) /CSM/VH/VHG1-D/VHG181Q.Seq.d/
AACAATAAAATTTTATTTTTCATTATTTATTTTTGATAAATTTTAAAGAATAAATATATA
TATATATATAAAATTATAATATGGCAAAAAAAAAGAGTAAAGAAACCAAATACTTGGGAT
CCAAATTATAATAAAAGTAATAAAAAATCCAAATTAGAATCAAATGAAAATAAAGTTGAA
ACTAATAATAATAATGAAATAATAATAGGTAATAATAGTGGTAGTGGTAATGATGCATAT
ATAACAACAGCAGGTGATAAAAAGATTGATAGTAANAACTTTTGGAATAATATTGAACCA
AAGAGTGAAATATTTGAACAATACTACAAGGCAATGGGTATCATTGATAATGAANAAGAG
TTTAAAAAGATGTTTGAATTAATGGGTAAACCATTACCAACCACATTTCGTATTAATACT
ACGATTGGAAAGAGTTTACAATCATTGGTAATCGATCAATTGAAAGAGTTGGTCAGTGAG
AGTAGTGGTTATAAAGACATTGAAATCGATGGTGAATTGGTTGAATTGCCATCACCATTT
TCATGGTATCCAAATGAAATGGCATGGAAATCGTCAATTCCAXXXXXXXXXXGATTTATT
GGGCATCAGATAGTGTTCTNTCAATCATTAGTAATGAATCCAATAACTCATTAAAGGTTA
TTAATTGNGCATTGAAATTATTCCAACGTCATGATGGTTTAGGTGCAATGGAATGTGCTT
ATANAATATCACAANATTNTGTACTTTGGATTGAACCATTNTTGAGCNAACGTATCATCA
CTATGACTCATGACGATTTGGTTATGATTTTCAAAAAGAATGAACCTTTNTTCACCNATT
TCAATGAATCAATTTGTGATCNATTAAAAGCTATGGAACCAGGTTGNTTCGTTATTAAAA
TTAGTGGTGCTTTACGTGAAACTNTATCATCAGGTATGGTTTTCTCTGCCTGGANAGGTA
AAGTTTCAATGCATCTTTTAGTTTCAAAACAAGAAATTGAATNTTATAAAGGTNTTTTCC
ANGTCATCGATGATCCAGTATCTCCAACTAATAATACTGCTATCGAATCAGTTAAAAAAG
ATGAATCAAAAGAAATTTCTTCTGAAATTGTCCAAGATCAACCNAAAGAACAAAAAG
sequence update 2002.10.25
Translated Amino Acid sequence 
nnkilffiiyf**ILKNKYIYIYKIIIWQKKRVKKPNTWDPNYNKSNKKSKLESNENKVE
TNNNNEIIIGNNSGSGNDAYITTAGDKKIDSXNFWNNIEPKSEIFEQYYKAMGIIDNEXE
FKKMFELMGKPLPTTFRINTTIGKSLQSLVIDQLKELVSESSGYKDIEIDGELVELPSPF
SWYPNEMAWKSSIP---

---IYWASDSVLSIISNESNNSLKVINXALKLFQRHDGLGAMECAYXISQXXVLWIEPXL
SXRIITMTHDDLVMIFKKNEPXFTXFNESICDXLKAMEPGXFVIKISGALRETXSSGMVF
SAWXGKVSMHLLVSKQEIEXYKGXFXVIDDPVSPTNNTAIESVKKDESKEISSEIVQDQP
KEQK
Translated Amino Acid sequence (All Frames) 
Frame A:
nnkilffiiyf**ILKNKYIYIYKIIIWQKKRVKKPNTWDPNYNKSNKKSKLESNENKVE
TNNNNEIIIGNNSGSGNDAYITTAGDKKIDSXNFWNNIEPKSEIFEQYYKAMGIIDNEXE
FKKMFELMGKPLPTTFRINTTIGKSLQSLVIDQLKELVSESSGYKDIEIDGELVELPSPF
SWYPNEMAWKSSIP---

---dllgir*csxnh***iq*likgy*lxieiipts*wfrcngmclxnitxxctld*tix
extyhhyds*rfgydfqke*tfxhxfq*inl*siksygtrlxry*n*wcft*nxiirygf
lclxr*sfnasfsfktrn*xl*rxfpxhr*ssisn**ycyris*kr*ikrnff*ncprst
xrtk

Frame B:
tikfyfslfifdkf*riniyiyikl*ygkkke*rnqilgiqiiikviknpn*nqmkiklk
liiimk***viivvvvmmhi*qqqvikrlivxtfgiilnqrvkylnnttrqwvslimxks
lkrcln*wvnhyqphfvlilrlervynhw*sin*kswsvrvvviktlksmvnwlnchhhf
hgiqmkwhgnrqf---

---IYWASDSVLSIISNESNNSLKVINXALKLFQRHDGLGAMECAYXISQXXVLWIEPXL
SXRIITMTHDDLVMIFKKNEPXFTXFNESICDXLKAMEPGXFVIKISGALRETXSSGMVF
SAWXGKVSMHLLVSKQEIEXYKGXFXVIDDPVSPTNNTAIESVKKDESKEISSEIVQDQP
KEQK

Frame C:
q*nfifhylflinfke*iyiyi*nynmakkksketkylgskl**k**kiqirik*k*s*n
*****nnnr***w*w**ciynnsr**kd***xlle*y*tke*ni*tilqgngyh***xrv
*kdv*ing*titnhisy*yydwkeftiignrsiervgq*e*wl*rh*nrw*ig*iaitif
mvsk*ngmeivns---

---fighqivfxqslvmnpith*rllixh*nysnvmmv*vqwnvlixyhxixyfglnhx*
anvssl*lmtiwl*fskrmnlxspismnqfvix*klwnqvxsllklvvlyvklyhqvwfs
lpgxvkfqcif*fqnkklnxikvfsxssmiqylqliillsnqlkkmnqkkfllklskinx
knkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHG181 (VHG181Q) /CSM/VH/VHG1-D/VHG181Q.Seq.d/                       1651   0.0
VHQ268 (VHQ268Q) /CSM/VH/VHQ2-C/VHQ268Q.Seq.d/                        934   0.0
VHI830 (VHI830Q) /CSM/VH/VHI8-B/VHI830Q.Seq.d/                        894   0.0
VHN606 (VHN606Q) /CSM/VH/VHN6-A/VHN606Q.Seq.d/                        890   0.0
VHC607 (VHC607Q) /CSM/VH/VHC6-A/VHC607Q.Seq.d/                        712   0.0
VSH441 (VSH441Q) /CSM/VS/VSH4-B/VSH441Q.Seq.d/                         36   1.1
VHL848 (VHL848Q) /CSM/VH/VHL8-B/VHL848Q.Seq.d/                         36   1.1
VHB858 (VHB858Q) /CSM/VH/VHB8-C/VHB858Q.Seq.d/                         36   1.1
VFN388 (VFN388Q) /CSM/VF/VFN3-D/VFN388Q.Seq.d/                         36   1.1
VFM765 (VFM765Q) /CSM/VF/VFM7-C/VFM765Q.Seq.d/                         36   1.1
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ440451) Dictyostelium discoideum cDNA clone:ddv43a21, 3' ...   977   0.0    1
(BJ446706) Dictyostelium discoideum cDNA clone:ddv63g18, 3' ...   864   0.0    3
(BJ441965) Dictyostelium discoideum cDNA clone:ddv48l08, 3' ...   872   0.0    2
(BJ445004) Dictyostelium discoideum cDNA clone:ddv58k02, 3' ...   775   0.0    4
(BJ427780) Dictyostelium discoideum cDNA clone:ddv63g18, 5' ...   658   0.0    2
(BJ419456) Dictyostelium discoideum cDNA clone:ddv36m02, 5' ...   658   0.0    2
(BJ421755) Dictyostelium discoideum cDNA clone:ddv43a21, 5' ...   658   0.0    2
(BJ423201) Dictyostelium discoideum cDNA clone:ddv48l08, 5' ...   650   0.0    2
(BJ426169) Dictyostelium discoideum cDNA clone:ddv58k02, 5' ...   644   0.0    2
(CQ481865) Sequence 13732 from Patent WO0160860.                   40   0.21   2
dna update 2008.11. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1HFZ0) RecName: Full=tRNA (cytosine-5-)-methyltransferase NSUN...    65   4e-09
AK150631_1(AK150631|pid:none) Mus musculus bone marrow macrophag...    65   4e-09
(Q28E61) RecName: Full=tRNA (cytosine-5-)-methyltransferase NSUN...    64   8e-09
(Q08J23) RecName: Full=tRNA (cytosine-5-)-methyltransferase NSUN...    64   1e-08
EF676449_1(EF676449|pid:none) Picea sitchensis clone WS02738_B19...    61   8e-08
CU928179_370(CU928179|pid:none) Zygosaccharomyces rouxii strain ...    60   2e-07
(Q5ZLV4) RecName: Full=tRNA (cytosine-5-)-methyltransferase NSUN...    60   2e-07
AK075999_1(AK075999|pid:none) Mus musculus 10 days embryo whole ...    57   9e-07
BC013625_1(BC013625|pid:none) Mus musculus NOL1/NOP2/Sun domain ...    57   9e-07
AY099759_1(AY099759|pid:none) Arabidopsis thaliana unknown prote...    57   9e-07
protein update 2009. 7. 6
PSORT 

 psg: 0.52  gvh: 0.03  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VHG181 is nuc
5' end seq. ID VHG181F
5' end seq. 
>VHG181F.Seq
AACAATAAAATTTTATTTTTCATTATTTATTTTTGATAAATTTTAAAGAATAAATATATA
TATATATATAAAATTATAATATGGCAAAAAAAAAGAGTAAAGAAACCAAATACTTGGGAT
CCAAATTATAATAAAAGTAATAAAAAATCCAAATTAGAATCAAATGAAAATAAAGTTGAA
ACTAATAATAATAATGAAATAATAATAGGTAATAATAGTGGTAGTGGTAATGATGCATAT
ATAACAACAGCAGGTGATAAAAAGATTGATAGTAANAACTTTTGGAATAATATTGAACCA
AAGAGTGAAATATTTGAACAATACTACAAGGCAATGGGTATCATTGATAATGAANAAGAG
TTTAAAAAGATGTTTGAATTAATGGGTAAACCATTACCAACCACATTTCGTATTAATACT
ACGATTGGAAAGAGTTTACAATCATTGGTAATCGATCAATTGAAAGAGTTGGTCAGTGAG
AGTAGTGGTTATAAAGACATTGAAATCGATGGTGAATTGGTTGAATTGCCATCACCATTT
TCATGGTATCCAAATGAAATGGCATGGAAATCGTCAATTCCANNNNNNNNNN
Length of 5' end seq. 592
3' end seq. ID VHG181Z
3' end seq. 
>VHG181Z.Seq
NNNNNNNNNNGATTTATTGGGCATCAGATAGTGTTCTNTCAATCATTAGTAATGAATCCA
ATAACTCATTAAAGGTTATTAATTGNGCATTGAAATTATTCCAACGTCATGATGGTTTAG
GTGCAATGGAATGTGCTTATANAATATCACAANATTNTGTACTTTGGATTGAACCATTNT
TGAGCNAACGTATCATCACTATGACTCATGACGATTTGGTTATGATTTTCAAAAAGAATG
AACCTTTNTTCACCNATTTCAATGAATCAATTTGTGATCNATTAAAAGCTATGGAACCAG
GTTGNTTCGTTATTAAAATTAGTGGTGCTTTACGTGAAACTNTATCATCAGGTATGGTTT
TCTCTGCCTGGANAGGTAAAGTTTCAATGCATCTTTTAGTTTCAAAACAAGAAATTGAAT
NTTATAAAGGTNTTTTCCANGTCATCGATGATCCAGTATCTCCAACTAATAATACTGCTA
TCGAATCAGTTAAAAAAGATGAATCAAAAGAAATTTCTTCTGAAATTGTCCAAGATCAAC
CNAAAGAACAAAAAG
Length of 3' end seq. 555
Connected seq. ID VHG181P
Connected seq. 
>VHG181P.Seq
AACAATAAAATTTTATTTTTCATTATTTATTTTTGATAAATTTTAAAGAATAAATATATA
TATATATATAAAATTATAATATGGCAAAAAAAAAGAGTAAAGAAACCAAATACTTGGGAT
CCAAATTATAATAAAAGTAATAAAAAATCCAAATTAGAATCAAATGAAAATAAAGTTGAA
ACTAATAATAATAATGAAATAATAATAGGTAATAATAGTGGTAGTGGTAATGATGCATAT
ATAACAACAGCAGGTGATAAAAAGATTGATAGTAANAACTTTTGGAATAATATTGAACCA
AAGAGTGAAATATTTGAACAATACTACAAGGCAATGGGTATCATTGATAATGAANAAGAG
TTTAAAAAGATGTTTGAATTAATGGGTAAACCATTACCAACCACATTTCGTATTAATACT
ACGATTGGAAAGAGTTTACAATCATTGGTAATCGATCAATTGAAAGAGTTGGTCAGTGAG
AGTAGTGGTTATAAAGACATTGAAATCGATGGTGAATTGGTTGAATTGCCATCACCATTT
TCATGGTATCCAAATGAAATGGCATGGAAATCGTCAATTCCA----------GATTTATT
GGGCATCAGATAGTGTTCTNTCAATCATTAGTAATGAATCCAATAACTCATTAAAGGTTA
TTAATTGNGCATTGAAATTATTCCAACGTCATGATGGTTTAGGTGCAATGGAATGTGCTT
ATANAATATCACAANATTNTGTACTTTGGATTGAACCATTNTTGAGCNAACGTATCATCA
CTATGACTCATGACGATTTGGTTATGATTTTCAAAAAGAATGAACCTTTNTTCACCNATT
TCAATGAATCAATTTGTGATCNATTAAAAGCTATGGAACCAGGTTGNTTCGTTATTAAAA
TTAGTGGTGCTTTACGTGAAACTNTATCATCAGGTATGGTTTTCTCTGCCTGGANAGGTA
AAGTTTCAATGCATCTTTTAGTTTCAAAACAAGAAATTGAATNTTATAAAGGTNTTTTCC
ANGTCATCGATGATCCAGTATCTCCAACTAATAATACTGCTATCGAATCAGTTAAAAAAG
ATGAATCAAAAGAAATTTCTTCTGAAATTGTCCAAGATCAACCNAAAGAACAAAAAG
Length of connected seq. 1127
Full length Seq ID -
Full length Seq. -
Length of full length seq. -