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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO265
Library AF
(Link to library)
Clone ID AFO265
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11680-1
Original site URL
Representative seq. ID AFO265E
(Link to Original site)
Representative DNA sequence 
>AFO265 (AFO265Q) /CSM/AF/AFO2-C/AFO265Q.Seq.d/
AGATGTTGGTATAATACCAGCTACAAATTGTCTTGACAAATCTTGTAAAACCATTTACAA
TTCATTATTTGATATTTATTATTCAGGTTGGACTTTTACAAGAGAATGGAGAGTTAATGA
AAATACTTGTTCAGATTTTGTAACATGTCCACAAATTAATTGTCAATCAACATTAAATCA
AAATTTAAATTGTAAATATTTATGTGATCAACAAGATTCTTTAGATTTTTGTGGTTATTG
TAGTAGTAGTAGTAGTGGTAGTATTGATAACACTACCACTACCAATAGTACATGTTTTAA
CTTTTTAGAATTAAATAATTCAATATCATGCGAAAATAGTGATCCAATTTGTATATTATC
AAATGGTGATTATTTATATAATCTTAGTAAAGAAGAATGTGAATTATCATCATCAATTGG
TAGTTGTACTCATTCATGTGGATTCGAATGTATTGGTGTTAGTAGACTTTGTATATTTGG
TGACTCTAATAAAACAACTTGTGAAAATAATGGTTACCTTTGGAATGGTGTTGATGGATA
TTGTGTTGATTTATCATCAACTACTGAAGAGCAATGTTATGGTAATTATAATGCATCTTC
TTCTTCCTCATCCTCTTCTCCCTCTTCTGATAGTCGTCCACTTTGGGTAGATTGTCCAAA
TAGATCAACAGATCTATGTAGTGATGGTTTCTTTTGTGATCTTAAAGAGGTACCATGTAA
AACTAAAGAACAATGTGAATCAATTGGTGGTGAATGTTCAGATATATTTTTCTTTAAACC
AGAGAATGTTCAATATTATCCATTTGGATTTGGTAAATGTGTTACAAATCATACATTCCT
TCAAAGAGATAATTCAAAGACAGTATCTTGTCCAAATGGTCAAGTTGATTCACCAAATGG
TTGTTTCTATCAATCACCGGTTTATAAAACAAAAGCTCAATGTTTAGATACACCAGGTAA
TAGATGGTGGACACCTTCAGATAATAGATCACAATGTCTATCGAATGAAAATCAAGGTTG
TAGAATAATGGATGTGGATTCATTTGCAAATTTACCATTTAATTTCATATTCAATGAAAT
GGATCAACATCAATGTGAAGGTTGTAGACAACTTTTACCAAGTTCCTCCAAATGGACTGA
TAAATTCAAATGGACAAATGCAACTTGGTTACCTGGTATTCATATTGATAAACCAAAATG
GTATAGAAATTTAACTTCAACCCCTCGTGTATTTAAATCAACATTTAACTATCAAAGTTT
TTATCTAGATTTAGTTGATGCAGTGGAAGACAAACTGCNATTTCTATAGATCTCAAACAT
T
sequence update 2001. 6. 9
Translated Amino Acid sequence 
DVGIIPATNCLDKSCKTIYNSLFDIYYSGWTFTREWRVNENTCSDFVTCPQINCQSTLNQ
NLNCKYLCDQQDSLDFCGYCSSSSSGSIDNTTTTNSTCFNFLELNNSISCENSDPICILS
NGDYLYNLSKEECELSSSIGSCTHSCGFECIGVSRLCIFGDSNKTTCENNGYLWNGVDGY
CVDLSSTTEEQCYGNYNASSSSSSSSPSSDSRPLWVDCPNRSTDLCSDGFFCDLKEVPCK
TKEQCESIGGECSDIFFFKPENVQYYPFGFGKCVTNHTFLQRDNSKTVSCPNGQVDSPNG
CFYQSPVYKTKAQCLDTPGNRWWTPSDNRSQCLSNENQGCRIMDVDSFANLPFNFIFNEM
DQHQCEGCRQLLPSSSKWTDKFKWTNATWLPGIHIDKPKWYRNLTSTPRVFKSTFNYQSF
YLDLVDAVEDKLXFL*isni
Translated Amino Acid sequence (All Frames) 
Frame A:
rcwyntsykls*qil*nhlqfii*yllfrldfykrmes**kylfrfcnmstn*lsiniks
kfkl*ifm*strffrflwll*****w*y**hyhyq*ymf*lfrik*fnimrk**snlyii
kw*lfi*s**rrm*iiiinw*lysfmwirmywc**tlyiw*l**nnl*k*wlplewc*wi
lc*fiiny*ramlw*l*ciffflilfslf**sstlgrlsk*inrsm**wfll*s*rgtm*
n*rtm*inww*mfryifl*trecsilsiwiw*mcyksyipskr*fkdsilskwss*ftkw
lflsitgl*nkssmfrytr**mvdtfr**itmsie*ksrl*nngcgfickfti*fhiq*n
gstsm*rl*ttftkflqmd**iqmdkcnlvtwysy**tkmv*kfnfnpsci*ini*lskf
lsrfs*csgrqtaisidlkh


Frame B:
DVGIIPATNCLDKSCKTIYNSLFDIYYSGWTFTREWRVNENTCSDFVTCPQINCQSTLNQ
NLNCKYLCDQQDSLDFCGYCSSSSSGSIDNTTTTNSTCFNFLELNNSISCENSDPICILS
NGDYLYNLSKEECELSSSIGSCTHSCGFECIGVSRLCIFGDSNKTTCENNGYLWNGVDGY
CVDLSSTTEEQCYGNYNASSSSSSSSPSSDSRPLWVDCPNRSTDLCSDGFFCDLKEVPCK
TKEQCESIGGECSDIFFFKPENVQYYPFGFGKCVTNHTFLQRDNSKTVSCPNGQVDSPNG
CFYQSPVYKTKAQCLDTPGNRWWTPSDNRSQCLSNENQGCRIMDVDSFANLPFNFIFNEM
DQHQCEGCRQLLPSSSKWTDKFKWTNATWLPGIHIDKPKWYRNLTSTPRVFKSTFNYQSF
YLDLVDAVEDKLXFL*isni


Frame C:
mlv*yqlqivltnlvkpftihylifiiqvgllqengelmkilvqil*hvhklivnqh*ik
i*ivniyvinkil*ifvvivvvvvvvvlitlplpivhvltf*n*iiqyhakiviqfvyyq
mviiyiilvkknvnyhhqlvvvlihvdsnvlvlvdfvylvtlikqlvkimvtfgmvlmdi
vliyhqllksnvmviimhlllphpllpllivvhfg*ivqidqqiyvvmvsfvilkryhvk
lknnvnqlvvnvqiyfslnqrmfniihldlvnvlqiihsfkeiiqrqylvqmvklihqmv
vsinhrfikqklnv*ihqvidgghlqiidhnvyrmkikvve*wmwihlqiyhlisysmkw
ininvkvvdnfyqvppnglinsngqmqlgylvfilinqngiei*lqplvylnqhltikvf
i*i*lmqwktncxfyrsqt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO265 (AFO265Q) /CSM/AF/AFO2-C/AFO265Q.Seq.d/                       2091   0.0
CHD516 (CHD516Q) /CSM/CH/CHD5-A/CHD516Q.Seq.d/                        341   2e-92
SLB372 (SLB372Q) /CSM/SL/SLB3-C/SLB372Q.Seq.d/                         48   3e-04
FC-BI07 (FC-BI07Q) /CSM/FC/FC-BI/FC-BI07Q.Seq.d/                       48   3e-04
CHP407 (CHP407Q) /CSM/CH/CHP4-A/CHP407Q.Seq.d/                         48   3e-04
CHN344 (CHN344Q) /CSM/CH/CHN3-B/CHN344Q.Seq.d/                         48   3e-04
VSF352 (VSF352Q) /CSM/VS/VSF3-C/VSF352Q.Seq.d/                         42   0.021
VHH223 (VHH223Q) /CSM/VH/VHH2-A/VHH223Q.Seq.d/                         42   0.021
VFD310 (VFD310Q) /CSM/VF/VFD3-A/VFD310Q.Seq.d/                         42   0.021
SSA747 (SSA747Q) /CSM/SS/SSA7-B/SSA747Q.Seq.d/                         42   0.021
own update 2002.10.30
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    48  2e-04  12
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    36  4e-04  11
AC110163|AC110163.7 Mus musculus clone RP23-134I10, LOW-PASS SEQUENCE SAMPLING.    56  0.001  1
AZ239043|AZ239043.1 RPCI-23-72G12.TV RPCI-23 Mus musculus genomic clone RPCI-23-72G12, DNA sequence.    56  0.001  1
AX720079|AX720079.1 Sequence 1 from Patent WO03023034.            56  0.001  1
BD183307|BD183307.1 Novel genes and proteins encoded by the genes.    56  0.001  1
AB058758|AB058758.1 Homo sapiens mRNA for KIAA1855 protein, partial cds.    56  0.001  1
AC116717|AC116717.3 Mus musculus clone RP23-198D7, WORKING DRAFT SEQUENCE, 3 ordered pieces.    54  0.005  1
AC122437|AC122437.2 Mus musculus chromosome UNK clone RP24-215N22, WORKING DRAFT SEQUENCE, 4 unordered pieces.    54  0.005  1
Z92547|Z92547.1 Human DNA sequence from PAC 863K19 on chromosome X. Contains STS.    52  0.007  2
dna update 2003. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE014187_405(AE014187|pid:none) Plasmodium falciparum 3D7 chromo...    40   0.14
CP001111_113(CP001111|pid:none) Stenotrophomonas maltophilia R55...    38   0.71
CR388098_1(CR388098|pid:none) Zebrafish DNA sequence from clone ...    37   1.2
DQ927305_30(DQ927305|pid:none) Tetrahymena pigmentosa strain UM1...    37   1.6
EU577816_2(EU577816|pid:none) HIV-1 isolate 4295.29 from USA vpu...    37   2.1
FB841880_1(FB841880|pid:none) Sequence 61153 from Patent WO20080...    37   2.1
EU577813_2(EU577813|pid:none) HIV-1 isolate 4295.24 from USA vpu...    36   2.7
BX510320_1(BX510320|pid:none) Zebrafish DNA sequence from clone ...    36   2.7
EU577822_2(EU577822|pid:none) HIV-1 isolate 4295.36 from USA vpu...    36   2.7
U40030_3(U40030|pid:none) Caenorhabditis elegans cosmid T13C2, c...    36   2.7
protein update 2009. 6.29
PSORT 

 psg: 0.75  gvh: 0.49  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.12  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFO265 is cyt
5' end seq. ID AFO265F
5' end seq. 
>AFO265F.Seq
AGATGTTGGTATAATACCAGCTACAAATTGTCTTGACAAATCTTGTAAAACCATTTACAA
TTCATTATTTGATATTTATTATTCAGGTTGGACTTTTACAAGAGAATGGAGAGTTAATGA
AAATACTTGTTCAGATTTTGTAACATGTCCACAAATTAATTGTCAATCAACATTAAATCA
AAATTTAAATTGTAAATATTTATGTGATCAACAAGATTCTTTAGATTTTTGTGGTTATTG
TAGTAGTAGTAGTAGTGGTAGTATTGATAACACTACCACTACCAATAGTACATGTTTTAA
CTTTTTAGAATTAAATAATTCAATATCATGCGAAAATAGTGATCCAATTTGTATATTATC
AAATGGTGATTATTTATATAATCTTAGTAAAGAAGAATGTGAATTATCATCATCAATTGG
TAGTTGTACTCATTCATGTGGATTCGAATGTATTGGTGTTAGTAGACTTTGTATATTTGG
TGACTCTAATAAAACAACTTGTGAAAATAATGGTTACCTTTGGAATGGTGTTGATGGATA
TTGTGTTGATTTATCATCAACTACTGAAGAGCAATGTTATGGTAATTATAATGCATCTTC
TTCTTCCTCATCCTCTTCTCCCTCTTCTGATAGTCGTCCACTTTGGGTAGATTG------
----
Length of 5' end seq. 654
3' end seq. ID AFO265Z
3' end seq. 
>AFO265Z.Seq
----------TCTTCTTCCTCATCCTCTTCTCCCTCTTCTGATAGTCGTCCACTTTGGGT
AGATTGTCCAAATAGATCAACAGATCTATGTAGTGATGGTTTCTTTTGTGATCTTAAAGA
GGTACCATGTAAAACTAAAGAACAATGTGAATCAATTGGTGGTGAATGTTCAGATATATT
TTTCTTTAAACCAGAGAATGTTCAATATTATCCATTTGGATTTGGTAAATGTGTTACAAA
TCATACATTCCTTCAAAGAGATAATTCAAAGACAGTATCTTGTCCAAATGGTCAAGTTGA
TTCACCAAATGGTTGTTTCTATCAATCACCGGTTTATAAAACAAAAGCTCAATGTTTAGA
TACACCAGGTAATAGATGGTGGACACCTTCAGATAATAGATCACAATGTCTATCGAATGA
AAATCAAGGTTGTAGAATAATGGATGTGGATTCATTTGCAAATTTACCATTTAATTTCAT
ATTCAATGAAATGGATCAACATCAATGTGAAGGTTGTAGACAACTTTTACCAAGTTCCTC
CAAATGGACTGATAAATTCAAATGGACAAATGCAACTTGGTTACCTGGTATTCATATTGA
TAAACCAAAATGGTATAGAAATTTAACTTCAACCCCTCGTGTATTTAAATCAACATTTAA
CTATCAAAGTTTTTATCTAGATTTAGTTGATGCAGTGGAAGACAAACTGCNATTTCTATA
GATCTCAAACATT
Length of 3' end seq. 723
Connected seq. ID AFO265P
Connected seq. 
>AFO265P.Seq
AGATGTTGGTATAATACCAGCTACAAATTGTCTTGACAAATCTTGTAAAACCATTTACAA
TTCATTATTTGATATTTATTATTCAGGTTGGACTTTTACAAGAGAATGGAGAGTTAATGA
AAATACTTGTTCAGATTTTGTAACATGTCCACAAATTAATTGTCAATCAACATTAAATCA
AAATTTAAATTGTAAATATTTATGTGATCAACAAGATTCTTTAGATTTTTGTGGTTATTG
TAGTAGTAGTAGTAGTGGTAGTATTGATAACACTACCACTACCAATAGTACATGTTTTAA
CTTTTTAGAATTAAATAATTCAATATCATGCGAAAATAGTGATCCAATTTGTATATTATC
AAATGGTGATTATTTATATAATCTTAGTAAAGAAGAATGTGAATTATCATCATCAATTGG
TAGTTGTACTCATTCATGTGGATTCGAATGTATTGGTGTTAGTAGACTTTGTATATTTGG
TGACTCTAATAAAACAACTTGTGAAAATAATGGTTACCTTTGGAATGGTGTTGATGGATA
TTGTGTTGATTTATCATCAACTACTGAAGAGCAATGTTATGGTAATTATAATGCATCTTC
TTCTTCCTCATCCTCTTCTCCCTCTTCTGATAGTCGTCCACTTTGGGTAGATTG------
----TCTTCTTCCTCATCCTCTTCTCCCTCTTCTGATAGTCGTCCACTTTGGGTAGATTG
TCCAAATAGATCAACAGATCTATGTAGTGATGGTTTCTTTTGTGATCTTAAAGAGGTACC
ATGTAAAACTAAAGAACAATGTGAATCAATTGGTGGTGAATGTTCAGATATATTTTTCTT
TAAACCAGAGAATGTTCAATATTATCCATTTGGATTTGGTAAATGTGTTACAAATCATAC
ATTCCTTCAAAGAGATAATTCAAAGACAGTATCTTGTCCAAATGGTCAAGTTGATTCACC
AAATGGTTGTTTCTATCAATCACCGGTTTATAAAACAAAAGCTCAATGTTTAGATACACC
AGGTAATAGATGGTGGACACCTTCAGATAATAGATCACAATGTCTATCGAATGAAAATCA
AGGTTGTAGAATAATGGATGTGGATTCATTTGCAAATTTACCATTTAATTTCATATTCAA
TGAAATGGATCAACATCAATGTGAAGGTTGTAGACAACTTTTACCAAGTTCCTCCAAATG
GACTGATAAATTCAAATGGACAAATGCAACTTGGTTACCTGGTATTCATATTGATAAACC
AAAATGGTATAGAAATTTAACTTCAACCCCTCGTGTATTTAAATCAACATTTAACTATCA
AAGTTTTTATCTAGATTTAGTTGATGCAGTGGAAGACAAACTGCNATTTCTATAGATCTC
AAACATT
Length of connected seq. 1377
Full length Seq ID AFO265E
Full length Seq. 
>AFO265E.Seq
AGATGTTGGTATAATACCAGCTACAAATTGTCTTGACAAATCTTGTAAAACCATTTACAA
TTCATTATTTGATATTTATTATTCAGGTTGGACTTTTACAAGAGAATGGAGAGTTAATGA
AAATACTTGTTCAGATTTTGTAACATGTCCACAAATTAATTGTCAATCAACATTAAATCA
AAATTTAAATTGTAAATATTTATGTGATCAACAAGATTCTTTAGATTTTTGTGGTTATTG
TAGTAGTAGTAGTAGTGGTAGTATTGATAACACTACCACTACCAATAGTACATGTTTTAA
CTTTTTAGAATTAAATAATTCAATATCATGCGAAAATAGTGATCCAATTTGTATATTATC
AAATGGTGATTATTTATATAATCTTAGTAAAGAAGAATGTGAATTATCATCATCAATTGG
TAGTTGTACTCATTCATGTGGATTCGAATGTATTGGTGTTAGTAGACTTTGTATATTTGG
TGACTCTAATAAAACAACTTGTGAAAATAATGGTTACCTTTGGAATGGTGTTGATGGATA
TTGTGTTGATTTATCATCAACTACTGAAGAGCAATGTTATGGTAATTATAATGCATCTTC
TTCTTCCTCATCCTCTTCTCCCTCTTCTGATAGTCGTCCACTTTGGGTAGATTGTCCAAA
TAGATCAACAGATCTATGTAGTGATGGTTTCTTTTGTGATCTTAAAGAGGTACCATGTAA
AACTAAAGAACAATGTGAATCAATTGGTGGTGAATGTTCAGATATATTTTTCTTTAAACC
AGAGAATGTTCAATATTATCCATTTGGATTTGGTAAATGTGTTACAAATCATACATTCCT
TCAAAGAGATAATTCAAAGACAGTATCTTGTCCAAATGGTCAAGTTGATTCACCAAATGG
TTGTTTCTATCAATCACCGGTTTATAAAACAAAAGCTCAATGTTTAGATACACCAGGTAA
TAGATGGTGGACACCTTCAGATAATAGATCACAATGTCTATCGAATGAAAATCAAGGTTG
TAGAATAATGGATGTGGATTCATTTGCAAATTTACCATTTAATTTCATATTCAATGAAAT
GGATCAACATCAATGTGAAGGTTGTAGACAACTTTTACCAAGTTCCTCCAAATGGACTGA
TAAATTCAAATGGACAAATGCAACTTGGTTACCTGGTATTCATATTGATAAACCAAAATG
GTATAGAAATTTAACTTCAACCCCTCGTGTATTTAAATCAACATTTAACTATCAAAGTTT
TTATCTAGATTTAGTTGATGCAGTGGAAGACAAACTGCNATTTCTATAGATCTCAAACAT
T
Length of full length seq. 1321