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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHL181
Library VH
(Link to library)
Clone ID VHL181
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15928-1
Original site URL
Representative seq. ID VHL181P
(Link to Original site)
Representative DNA sequence 
>VHL181 (VHL181Q) /CSM/VH/VHL1-D/VHL181Q.Seq.d/
AAAAAGTAAAAATAAAATATTGAAAATGGAAGAGATACAAAAATTAATTAATGAATTAGG
TGGTTCACAATATAAAGATAAAGATACAGAACATTATTCTGAAGAAGCAGTAGAGTTATT
AATTAAAAAGATTGATAATAAATCAAAAGGGAAAACAGTTATTGAAAGTAATACATTANA
TTTATAAGACATTAGCAGATTGGTCAAAAGTTGAAGATAATAGAGAAACAATCATTAGAT
CACCATCAAATGTAATTGAAAAAACTTTTCAATTATTTGAATTCTTATTACCAAATGGCG
TAGAAACATTAAATACAGATACTACAGTTGAACAATCAGAATTATTTTCAATGGTTTGTA
GATTACTTGGTAATTTAACCTATGAAAATGCACCAAATCGTGAATTTATCTTTGATAAAA
CTCCTTCCATTTTAAAATATATTTCAAGTTTTGTATCTCAAACAAAGTATCAATCATTAC
AAAGAACTTCTTGTGCTGCCATTGCAAATCTTTCTTCTGAAACTGATTTTATTCAACTTG
AATTCTTTAAATTAGGAGTAGTTTCAATTTTAATCGATTTAATTTGTAGAGAAGGTACAA
CTGATGAAGTTAATCAAATGGCAATTAAAGCATTTAATAATTTAGTTGATAATGAAAATA
CXXXXXXXXXXGAACAATTACAATTAGATGATGCTAAAATTCAAGAAATTGAAGAGAAAT
TTGAAGAAAAAGAAAAAACAATTGAAAAAACAGATGAAAAAACAGATGAAAAAACAAATG
AAAAGAAACAATCAAAGAAAGATATGAGAGTAATTTATGAAGCAACTAGATTATTACTTA
GATTTTTAGATAATTCTGAATTATCAAATCATCAAGAGAAAATGAAACAATTGATTGAGG
AATCAGTTGAACCATTCTTTAGTTTATTACAATCTCCTTTCCCAATTTTACAAGTTGAAG
GTGCAAAAGGTTTAGTTTTACTCATTAAACATGATAAACAACTCTTTTTATCACGTCCAT
CATGGGTAAAAGATTTGGTTGAAGTTTTATCCCTTTCAATCATTCCTTTCCAACAACTCT
CAAGAGAACAATCAACAATTACTACCGATCCAAATCATCAAAAATTAATTGCACAAATTC
AATCAAATTGTAATTTTAGTACCGAACTTCAAAATGTTATCCTTTCAAATATTTTTTACA
ATTTCTCTTTAGATGAATCAATTTGTAAAAAAATGAAAGATCAAAATGTTATTTCNAATT
AAAAACTTTA
sequence update 2002.10.25
Translated Amino Acid sequence 
kvkiky*kwkrykn*lmn*vvhnikikiqniilkkq*sy*LKRLIINQKGKQLLKVIHXI
YKTLADWSKVEDNRETIIRSPSNVIEKTFQLFEFLLPNGVETLNTDTTVEQSELFSMVCR
LLGNLTYENAPNREFIFDKTPSILKYISSFVSQTKYQSLQRTSCAAIANLSSETDFIQLE
FFKLGVVSILIDLICREGTTDEVNQMAIKAFNNLVDNEN---

---EQLQLDDAKIQEIEEKFEEKEKTIEKTDEKTDEKTNEKKQSKKDMRVIYEATRLLLR
FLDNSELSNHQEKMKQLIEESVEPFFSLLQSPFPILQVEGAKGLVLLIKHDKQLFLSRPS
WVKDLVEVLSLSIIPFQQLSREQSTITTDPNHQKLIAQIQSNCNFSTELQNVILSNIFYN
FSLDESICKKMKDQNVISN*kl
Translated Amino Acid sequence (All Frames) 
Frame A:
kk*k*niengrdtkin**irwfti*r*ryrtlf*rssrvin*kd***ikrensy*k*yix
firh*qigqklkiiekqsldhhqm*lkklfnylnsyyqma*kh*iqilqlnnqnyfqwfv
dylvi*pmkmhqivnlslikllpf*nifqvlylkqsinhykellvlplqifllklilfnl
nsln*e*fqf*si*fvekvqlmklikwqlkhlii*limki---

---EQLQLDDAKIQEIEEKFEEKEKTIEKTDEKTDEKTNEKKQSKKDMRVIYEATRLLLR
FLDNSELSNHQEKMKQLIEESVEPFFSLLQSPFPILQVEGAKGLVLLIKHDKQLFLSRPS
WVKDLVEVLSLSIIPFQQLSREQSTITTDPNHQKLIAQIQSNCNFSTELQNVILSNIFYN
FSLDESICKKMKDQNVISN*kl

Frame B:
ksknkilkmeeiqklinelggsqykdkdtehyseeavellikkidnkskgktviesntlx
l*disrlvks*r**rnnh*itikcn*knfsii*ilitkwrrnikyryys*tiriifngl*
itw*fnl*kctks*iyl**nsfhfkiyfkfcisnkvsiitknflcchcksff*n*fyst*
il*irssfnfnrfnl*rryn**s*sngn*si**fs***ky---

---nnyn*mmlkfkklkrnlkkkkkqlkkqmkkqmkkqmkrnnqrki*e*fmkqldyyld
f*iilnyqiikrk*nn*lrnqlnhslvyynllsqfyklkvqkv*fyslnminnsfyhvhh
g*kiwlkfypfqsflsnnsqennqqllpiqiikn*lhkfnqivilvpnfkmlsfqiffti
sl*mnqfvkk*kikmlfxiknf

Frame C:
kvkiky*kwkrykn*lmn*vvhnikikiqniilkkq*sy*LKRLIINQKGKQLLKVIHXI
YKTLADWSKVEDNRETIIRSPSNVIEKTFQLFEFLLPNGVETLNTDTTVEQSELFSMVCR
LLGNLTYENAPNREFIFDKTPSILKYISSFVSQTKYQSLQRTSCAAIANLSSETDFIQLE
FFKLGVVSILIDLICREGTTDEVNQMAIKAFNNLVDNEN---

---titir*c*nsrn*rei*rkrknn*knr*knr*knk*ketikeryesnl*sn*iit*i
fr*f*iikssrenetid*gis*til*fitisfpnfts*rckrfsfth*t**ttlfitsim
gkrfg*sfipfnhsfpttlkrtinnyyrsksskinctnsikl*f*yrtskcypfkyflqf
lfr*inl*knerskcyfxlktl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL181 (VHL181Q) /CSM/VH/VHL1-D/VHL181Q.Seq.d/                       1245   0.0
VHB203 (VHB203Q) /CSM/VH/VHB2-A/VHB203Q.Seq.d/                       1209   0.0
CFJ429 (CFJ429Q) /CSM/CF/CFJ4-B/CFJ429Q.Seq.d/                       1209   0.0
SFJ725 (SFJ725Q) /CSM/SF/SFJ7-B/SFJ725Q.Seq.d/                       1207   0.0
CHG878 (CHG878Q) /CSM/CH/CHG8-D/CHG878Q.Seq.d/                       1203   0.0
CHK868 (CHK868Q) /CSM/CH/CHK8-C/CHK868Q.Seq.d/                       1201   0.0
CHG791 (CHG791Q) /CSM/CH/CHG7-D/CHG791Q.Seq.d/                       1199   0.0
CHQ791 (CHQ791Q) /CSM/CH/CHQ7-D/CHQ791Q.Seq.d/                       1148   0.0
CHQ524 (CHQ524Q) /CSM/CH/CHQ5-A/CHQ524Q.Seq.d/                       1132   0.0
CHR291 (CHR291Q) /CSM/CH/CHR2-D/CHR291Q.Seq.d/                       1118   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ424832) Dictyostelium discoideum cDNA clone:ddv53a21, 5' ...  1191   0.0    1
(BJ389837) Dictyostelium discoideum cDNA clone:dds20b07, 5' ...  1179   0.0    1
(BJ418523) Dictyostelium discoideum cDNA clone:ddv33e02, 5' ...   955   0.0    2
(BJ365594) Dictyostelium discoideum cDNA clone:ddc35l20, 5' ...   950   0.0    2
(BJ367679) Dictyostelium discoideum cDNA clone:ddc43h18, 5' ...   948   0.0    2
(BJ365571) Dictyostelium discoideum cDNA clone:ddc35f23, 5' ...   946   0.0    2
(BJ361926) Dictyostelium discoideum cDNA clone:ddc19j08, 5' ...   904   0.0    2
(BJ371133) Dictyostelium discoideum cDNA clone:ddc56e24, 5' ...   864   0.0    2
(BJ419087) Dictyostelium discoideum cDNA clone:ddv35b04, 5' ...   862   0.0    2
(BJ368986) Dictyostelium discoideum cDNA clone:ddc48h19, 5' ...   862   0.0    2
dna update 2008.11.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O93614) RecName: Full=Rap1 GTPase-GDP dissociation stimulator 1...    54   9e-06
JE0358(JE0358)ralB binding protein - clawed frog                       54   9e-06
AJ719848_1(AJ719848|pid:none) Gallus gallus mRNA for hypothetica...    45   0.006
AK160989_1(AK160989|pid:none) Mus musculus 15 days embryo head c...    44   0.016
AK172052_1(AK172052|pid:none) Mus musculus activated spleen cDNA...    44   0.016
BC098269_1(BC098269|pid:none) Homo sapiens RAP1, GTP-GDP dissoci...    43   0.021
AK055032_1(AK055032|pid:none) Homo sapiens cDNA FLJ30470 fis, cl...    43   0.021
AF215923_1(AF215923|pid:none) Homo sapiens exchange factor smgGD...    43   0.021
AY889292_1(AY889292|pid:none) Synthetic construct Homo sapiens c...    43   0.021
CP000749_2548(CP000749|pid:none) Marinomonas sp. MWYL1, complete...    36   2.6
protein update 2009. 7.15
PSORT 

 psg: 0.71  gvh: 0.46  alm: 0.30  top: 0.47  tms: 0.07  mit: 0.39  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.11  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: mitochondrial
	 24.0 %: nuclear
	 12.0 %: Golgi
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  4.0 %: vacuolar

>> prediction for VHL181 is mit
5' end seq. ID VHL181F
5' end seq. 
>VHL181F.Seq
AAAAAGTAAAAATAAAATATTGAAAATGGAAGAGATACAAAAATTAATTAATGAATTAGG
TGGTTCACAATATAAAGATAAAGATACAGAACATTATTCTGAAGAAGCAGTAGAGTTATT
AATTAAAAAGATTGATAATAAATCAAAAGGGAAAACAGTTATTGAAAGTAATACATTANA
TTTATAAGACATTAGCAGATTGGTCAAAAGTTGAAGATAATAGAGAAACAATCATTAGAT
CACCATCAAATGTAATTGAAAAAACTTTTCAATTATTTGAATTCTTATTACCAAATGGCG
TAGAAACATTAAATACAGATACTACAGTTGAACAATCAGAATTATTTTCAATGGTTTGTA
GATTACTTGGTAATTTAACCTATGAAAATGCACCAAATCGTGAATTTATCTTTGATAAAA
CTCCTTCCATTTTAAAATATATTTCAAGTTTTGTATCTCAAACAAAGTATCAATCATTAC
AAAGAACTTCTTGTGCTGCCATTGCAAATCTTTCTTCTGAAACTGATTTTATTCAACTTG
AATTCTTTAAATTAGGAGTAGTTTCAATTTTAATCGATTTAATTTGTAGAGAAGGTACAA
CTGATGAAGTTAATCAAATGGCAATTAAAGCATTTAATAATTTAGTTGATAATGAAAATA
CNNNNNNNNNN
Length of 5' end seq. 671
3' end seq. ID VHL181Z
3' end seq. 
>VHL181Z.Seq
NNNNNNNNNNGAACAATTACAATTAGATGATGCTAAAATTCAAGAAATTGAAGAGAAATT
TGAAGAAAAAGAAAAAACAATTGAAAAAACAGATGAAAAAACAGATGAAAAAACAAATGA
AAAGAAACAATCAAAGAAAGATATGAGAGTAATTTATGAAGCAACTAGATTATTACTTAG
ATTTTTAGATAATTCTGAATTATCAAATCATCAAGAGAAAATGAAACAATTGATTGAGGA
ATCAGTTGAACCATTCTTTAGTTTATTACAATCTCCTTTCCCAATTTTACAAGTTGAAGG
TGCAAAAGGTTTAGTTTTACTCATTAAACATGATAAACAACTCTTTTTATCACGTCCATC
ATGGGTAAAAGATTTGGTTGAAGTTTTATCCCTTTCAATCATTCCTTTCCAACAACTCTC
AAGAGAACAATCAACAATTACTACCGATCCAAATCATCAAAAATTAATTGCACAAATTCA
ATCAAATTGTAATTTTAGTACCGAACTTCAAAATGTTATCCTTTCAAATATTTTTTACAA
TTTCTCTTTAGATGAATCAATTTGTAAAAAAATGAAAGATCAAAATGTTATTTCNAATTA
AAAACTTTA
Length of 3' end seq. 609
Connected seq. ID VHL181P
Connected seq. 
>VHL181P.Seq
AAAAAGTAAAAATAAAATATTGAAAATGGAAGAGATACAAAAATTAATTAATGAATTAGG
TGGTTCACAATATAAAGATAAAGATACAGAACATTATTCTGAAGAAGCAGTAGAGTTATT
AATTAAAAAGATTGATAATAAATCAAAAGGGAAAACAGTTATTGAAAGTAATACATTANA
TTTATAAGACATTAGCAGATTGGTCAAAAGTTGAAGATAATAGAGAAACAATCATTAGAT
CACCATCAAATGTAATTGAAAAAACTTTTCAATTATTTGAATTCTTATTACCAAATGGCG
TAGAAACATTAAATACAGATACTACAGTTGAACAATCAGAATTATTTTCAATGGTTTGTA
GATTACTTGGTAATTTAACCTATGAAAATGCACCAAATCGTGAATTTATCTTTGATAAAA
CTCCTTCCATTTTAAAATATATTTCAAGTTTTGTATCTCAAACAAAGTATCAATCATTAC
AAAGAACTTCTTGTGCTGCCATTGCAAATCTTTCTTCTGAAACTGATTTTATTCAACTTG
AATTCTTTAAATTAGGAGTAGTTTCAATTTTAATCGATTTAATTTGTAGAGAAGGTACAA
CTGATGAAGTTAATCAAATGGCAATTAAAGCATTTAATAATTTAGTTGATAATGAAAATA
C----------GAACAATTACAATTAGATGATGCTAAAATTCAAGAAATTGAAGAGAAAT
TTGAAGAAAAAGAAAAAACAATTGAAAAAACAGATGAAAAAACAGATGAAAAAACAAATG
AAAAGAAACAATCAAAGAAAGATATGAGAGTAATTTATGAAGCAACTAGATTATTACTTA
GATTTTTAGATAATTCTGAATTATCAAATCATCAAGAGAAAATGAAACAATTGATTGAGG
AATCAGTTGAACCATTCTTTAGTTTATTACAATCTCCTTTCCCAATTTTACAAGTTGAAG
GTGCAAAAGGTTTAGTTTTACTCATTAAACATGATAAACAACTCTTTTTATCACGTCCAT
CATGGGTAAAAGATTTGGTTGAAGTTTTATCCCTTTCAATCATTCCTTTCCAACAACTCT
CAAGAGAACAATCAACAATTACTACCGATCCAAATCATCAAAAATTAATTGCACAAATTC
AATCAAATTGTAATTTTAGTACCGAACTTCAAAATGTTATCCTTTCAAATATTTTTTACA
ATTTCTCTTTAGATGAATCAATTTGTAAAAAAATGAAAGATCAAAATGTTATTTCNAATT
AAAAACTTTA
Length of connected seq. 1260
Full length Seq ID -
Full length Seq. -
Length of full length seq. -