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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHO482
Library VH
(Link to library)
Clone ID VHO482
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16427-1
Original site URL
Representative seq. ID VHO482P
(Link to Original site)
Representative DNA sequence 
>VHO482 (VHO482Q) /CSM/VH/VHO4-D/VHO482Q.Seq.d/
AATTTATTCATTGTGTGATTGTGTTGTGTGTGTATTTTGGTTGATACACATTATATATAT
ATATAATACAATAAAATGTCATTTTTTTTAGGACAGATTTTAGAAAAGATGGGTTCCATC
GATAAAGATATTAGATTCATGGCAACTCATGATCTTGCAAATGAATTAGAAAAAGATACT
TTTAAAATGGATCCAACTTATGAAAATAAAATTGTTACAAAATTATTAGCATTAACAGCT
GATTCAGCAAATAATGTTCAAGAAAATGTTGTTAAATGTTTAGGTTTATTAATTAAAAGA
GTTAAAGATTCACAAGCAACAGAAATTATTGATACATTATCAAAAAATATTTTAGAAGAA
TCAAATAAAGAAGAATTAGTTGAAATTTCAGGTATTGGTTTAAAAACAATCATTACAAAT
TTACCAAGTGAAGGTAGTTCAATTTCAACTTTAGTAATTAAAAATTTAGTACCAAAATTA
TTAATTGGTATTGATAGTGAAAAATTAAAAGATAAAAATGAAATTAAAATGTCATGTTTA
GATATTTTAAATGATTTATTACAAAAGTATGGATCATTTATGATTGGAGATTTAGAGXXX
XXXXXXXCCAAACATCTNTCAATTCCTTTCACTCTTACATGATGGTGATTTAATTGTTAG
ACGTAGTGCCCTCCTTTCATTGAATTACATTGCTCACAATAAACCAAATCTCATTCGTAA
TGATCTCTCTGTCTATTTACCAATCTTGTACAACAATGCTAAAATCAAACCAGAACTCAT
TCGTGAAGTTGATCTTGGTCCATTCAAACATAAGGTCGATGATGGTATTGAAATCAGAAA
AACAGCTTTCGAATGTATGTACACTCTTTTGGATACCTCTATCGATAAGATCGATGTAGC
TCCATTCATCGTTTCATTATGTGATGGTCTCAAAGATACCCAATACGATATCAAATTGTT
ATGTCATTTAATGATCATTCGTTTAGCAAACTCAAATGGTGCTGCTCTCCTCGAAAATAT
TACTCTCCTCCTTGAACCACTTCGTGTTATTCTCATGACTAAAGTAAATGAAACCGCTGT
TAAACAACAAATCGAACGTAATGAAGAATGTATTCGTTCAGCTTTACGTGCTGTCGCTTC
CATCTCTAGAATTCCAAATAGTGATAGTATCGTTAAATTTGAAGAATTTGTTAAAAATAC
AATTCGTACAACTCCATTAGCTGCTCAATTTAATTCAATTTTATC
sequence update 2002.10.25
Translated Amino Acid sequence 
nlfiv*lccvcilvdthyiyi*YNKMSFFLGQILEKMGSIDKDIRFMATHDLANELEKDT
FKMDPTYENKIVTKLLALTADSANNVQENVVKCLGLLIKRVKDSQATEIIDTLSKNILEE
SNKEELVEISGIGLKTIITNLPSEGSSISTLVIKNLVPKLLIGIDSEKLKDKNEIKMSCL
DILNDLLQKYGSFMIGDLE---

---PNIXQFLSLLHDGDLIVRRSALLSLNYIAHNKPNLIRNDLSVYLPILYNNAKIKPEL
IREVDLGPFKHKVDDGIEIRKTAFECMYTLLDTSIDKIDVAPFIVSLCDGLKDTQYDIKL
LCHLMIIRLANSNGAALLENITLLLEPLRVILMTKVNETAVKQQIERNEECIRSALRAVA
SISRIPNSDSIVKFEEFVKNTIRTTPLAAQFNSIL
Translated Amino Acid sequence (All Frames) 
Frame A:
nlfiv*lccvcilvdthyiyi*YNKMSFFLGQILEKMGSIDKDIRFMATHDLANELEKDT
FKMDPTYENKIVTKLLALTADSANNVQENVVKCLGLLIKRVKDSQATEIIDTLSKNILEE
SNKEELVEISGIGLKTIITNLPSEGSSISTLVIKNLVPKLLIGIDSEKLKDKNEIKMSCL
DILNDLLQKYGSFMIGDLE---

---PNIXQFLSLLHDGDLIVRRSALLSLNYIAHNKPNLIRNDLSVYLPILYNNAKIKPEL
IREVDLGPFKHKVDDGIEIRKTAFECMYTLLDTSIDKIDVAPFIVSLCDGLKDTQYDIKL
LCHLMIIRLANSNGAALLENITLLLEPLRVILMTKVNETAVKQQIERNEECIRSALRAVA
SISRIPNSDSIVKFEEFVKNTIRTTPLAAQFNSIL

Frame B:
iyslcdcvvcvfwlihiiyiyntikchff*drf*krwvpsikildswqlmilqmn*kkil
lkwiqlmkikllqny*h*qliqqimfkkmllnv*vy*lkelkihkqqkllihyqkif*kn
qikkn*lkfqvlv*kqslqiyqvkvvqfql**lki*yqny*lvlivkn*kikmklkchv*
if*miyyksmdhl*lei*---

---qtsxnsfhsymmvi*lldvvpsfh*itlltinqisfvmislsiyqscttmlksnqns
fvklilvhsnirsmmvlksekqlsnvctlfwiplsirsm*lhssfhyvmvskipntisnc
yvi**sfv*qtqmvllsskillsslnhfvlfs*lk*mkpllnnksnvmknvfvqlyvlsl
pslefqivivslnlknllkiqfvqlh*llnliqfy

Frame C:
fihcvivlcvyfg*ytlyiyiiq*nviffrtdfrkdgfhr*ry*ihgns*sck*irkryf
*ngsnl*k*ncykiisins*fsk*csrkcc*mfrfin*ks*rftsnrny*yiikkyfrri
k*rris*nfrywfknnhykftk*r*fnfnfsn*kfstkiinwy***kikr*k*n*nvmfr
yfk*fitkvwiiydwrfr---

---khlsipftlt*w*fnc*t*cppfielhcsq*tkshs**slclftnlvqqc*nqtrth
s*s*swsiqt*gr*wy*nqknsfrmyvhsfgylyr*drcssihrfim*wsqrypiryqiv
msfndhsfsklkwccsprkyyspp*ttscyshd*sk*nrc*ttnrt**rmysfsftccrf
hl*nsk***yr*i*ric*kynsynsiscsi*fnfi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO482 (VHO482Q) /CSM/VH/VHO4-D/VHO482Q.Seq.d/                       1352   0.0
VHQ485 (VHQ485Q) /CSM/VH/VHQ4-D/VHQ485Q.Seq.d/                       1259   0.0
VHC193 (VHC193Q) /CSM/VH/VHC1-D/VHC193Q.Seq.d/                       1259   0.0
VHB862 (VHB862Q) /CSM/VH/VHB8-C/VHB862Q.Seq.d/                       1259   0.0
SHL726 (SHL726Q) /CSM/SH/SHL7-B/SHL726Q.Seq.d/                       1259   0.0
SHJ830 (SHJ830Q) /CSM/SH/SHJ8-B/SHJ830Q.Seq.d/                       1259   0.0
SHJ681 (SHJ681Q) /CSM/SH/SHJ6-D/SHJ681Q.Seq.d/                       1259   0.0
SHG746 (SHG746Q) /CSM/SH/SHG7-B/SHG746Q.Seq.d/                       1259   0.0
SHG570 (SHG570Q) /CSM/SH/SHG5-C/SHG570Q.Seq.d/                       1259   0.0
SHF352 (SHF352Q) /CSM/SH/SHF3-C/SHF352Q.Seq.d/                       1259   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116956) Dictyostelium discoideum chromosome 2 map 1418423...  1259   0.0    1
(BJ446800) Dictyostelium discoideum cDNA clone:ddv63j22, 3' ...  1259   0.0    1
(BJ445539) Dictyostelium discoideum cDNA clone:ddv59d22, 3' ...  1259   0.0    1
(BJ437942) Dictyostelium discoideum cDNA clone:ddv35i23, 3' ...  1259   0.0    1
(BJ437564) Dictyostelium discoideum cDNA clone:ddv34l16, 3' ...  1259   0.0    1
(BJ408879) Dictyostelium discoideum cDNA clone:dds47d07, 3' ...  1259   0.0    1
(BJ407818) Dictyostelium discoideum cDNA clone:dds43a22, 3' ...  1259   0.0    1
(BJ407711) Dictyostelium discoideum cDNA clone:dds43l08, 3' ...  1259   0.0    1
(BJ407156) Dictyostelium discoideum cDNA clone:dds37k17, 3' ...  1259   0.0    1
(BJ407078) Dictyostelium discoideum cDNA clone:dds37l11, 3' ...  1259   0.0    1
dna update 2008.12.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86KD1) RecName: Full=Cullin-associated NEDD8-dissociated prote...   385   e-105
BC004232_1(BC004232|pid:none) Homo sapiens cullin-associated and...   183   1e-44
AK001791_1(AK001791|pid:none) Homo sapiens cDNA FLJ10929 fis, cl...   183   1e-44
AB179357_1(AB179357|pid:none) Macaca fascicularis testis cDNA cl...   183   1e-44
BC026220_1(BC026220|pid:none) Homo sapiens cullin-associated and...   183   1e-44
AB020636_1(AB020636|pid:none) Homo sapiens mRNA for KIAA0829 pro...   183   1e-44
AK292456_1(AK292456|pid:none) Homo sapiens cDNA FLJ77762 complet...   183   1e-44
AL833880_1(AL833880|pid:none) Homo sapiens mRNA; cDNA DKFZp434P1...   183   1e-44
(A7MBJ5) RecName: Full=Cullin-associated NEDD8-dissociated prote...   183   1e-44
(Q6ZQ38) RecName: Full=Cullin-associated NEDD8-dissociated prote...   182   2e-44
protein update 2009. 7.19
PSORT 

 psg: 0.90  gvh: 0.44  alm: 0.36  top: 0.40  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: vesicles of secretory system
	  8.0 %: endoplasmic reticulum

>> prediction for VHO482 is cyt
5' end seq. ID VHO482F
5' end seq. 
>VHO482F.Seq
AATTTATTCATTGTGTGATTGTGTTGTGTGTGTATTTTGGTTGATACACATTATATATAT
ATATAATACAATAAAATGTCATTTTTTTTAGGACAGATTTTAGAAAAGATGGGTTCCATC
GATAAAGATATTAGATTCATGGCAACTCATGATCTTGCAAATGAATTAGAAAAAGATACT
TTTAAAATGGATCCAACTTATGAAAATAAAATTGTTACAAAATTATTAGCATTAACAGCT
GATTCAGCAAATAATGTTCAAGAAAATGTTGTTAAATGTTTAGGTTTATTAATTAAAAGA
GTTAAAGATTCACAAGCAACAGAAATTATTGATACATTATCAAAAAATATTTTAGAAGAA
TCAAATAAAGAAGAATTAGTTGAAATTTCAGGTATTGGTTTAAAAACAATCATTACAAAT
TTACCAAGTGAAGGTAGTTCAATTTCAACTTTAGTAATTAAAAATTTAGTACCAAAATTA
TTAATTGGTATTGATAGTGAAAAATTAAAAGATAAAAATGAAATTAAAATGTCATGTTTA
GATATTTTAAATGATTTATTACAAAAGTATGGATCATTTATGATTGGAGATTTAGAGNNN
NNNNNNN
Length of 5' end seq. 607
3' end seq. ID VHO482Z
3' end seq. 
>VHO482Z.Seq
NNNNNNNNNNCCAAACATCTNTCAATTCCTTTCACTCTTACATGATGGTGATTTAATTGT
TAGACGTAGTGCCCTCCTTTCATTGAATTACATTGCTCACAATAAACCAAATCTCATTCG
TAATGATCTCTCTGTCTATTTACCAATCTTGTACAACAATGCTAAAATCAAACCAGAACT
CATTCGTGAAGTTGATCTTGGTCCATTCAAACATAAGGTCGATGATGGTATTGAAATCAG
AAAAACAGCTTTCGAATGTATGTACACTCTTTTGGATACCTCTATCGATAAGATCGATGT
AGCTCCATTCATCGTTTCATTATGTGATGGTCTCAAAGATACCCAATACGATATCAAATT
GTTATGTCATTTAATGATCATTCGTTTAGCAAACTCAAATGGTGCTGCTCTCCTCGAAAA
TATTACTCTCCTCCTTGAACCACTTCGTGTTATTCTCATGACTAAAGTAAATGAAACCGC
TGTTAAACAACAAATCGAACGTAATGAAGAATGTATTCGTTCAGCTTTACGTGCTGTCGC
TTCCATCTCTAGAATTCCAAATAGTGATAGTATCGTTAAATTTGAAGAATTTGTTAAAAA
TACAATTCGTACAACTCCATTAGCTGCTCAATTTAATTCAATTTTATC
Length of 3' end seq. 648
Connected seq. ID VHO482P
Connected seq. 
>VHO482P.Seq
AATTTATTCATTGTGTGATTGTGTTGTGTGTGTATTTTGGTTGATACACATTATATATAT
ATATAATACAATAAAATGTCATTTTTTTTAGGACAGATTTTAGAAAAGATGGGTTCCATC
GATAAAGATATTAGATTCATGGCAACTCATGATCTTGCAAATGAATTAGAAAAAGATACT
TTTAAAATGGATCCAACTTATGAAAATAAAATTGTTACAAAATTATTAGCATTAACAGCT
GATTCAGCAAATAATGTTCAAGAAAATGTTGTTAAATGTTTAGGTTTATTAATTAAAAGA
GTTAAAGATTCACAAGCAACAGAAATTATTGATACATTATCAAAAAATATTTTAGAAGAA
TCAAATAAAGAAGAATTAGTTGAAATTTCAGGTATTGGTTTAAAAACAATCATTACAAAT
TTACCAAGTGAAGGTAGTTCAATTTCAACTTTAGTAATTAAAAATTTAGTACCAAAATTA
TTAATTGGTATTGATAGTGAAAAATTAAAAGATAAAAATGAAATTAAAATGTCATGTTTA
GATATTTTAAATGATTTATTACAAAAGTATGGATCATTTATGATTGGAGATTTAGAG---
-------CCAAACATCTNTCAATTCCTTTCACTCTTACATGATGGTGATTTAATTGTTAG
ACGTAGTGCCCTCCTTTCATTGAATTACATTGCTCACAATAAACCAAATCTCATTCGTAA
TGATCTCTCTGTCTATTTACCAATCTTGTACAACAATGCTAAAATCAAACCAGAACTCAT
TCGTGAAGTTGATCTTGGTCCATTCAAACATAAGGTCGATGATGGTATTGAAATCAGAAA
AACAGCTTTCGAATGTATGTACACTCTTTTGGATACCTCTATCGATAAGATCGATGTAGC
TCCATTCATCGTTTCATTATGTGATGGTCTCAAAGATACCCAATACGATATCAAATTGTT
ATGTCATTTAATGATCATTCGTTTAGCAAACTCAAATGGTGCTGCTCTCCTCGAAAATAT
TACTCTCCTCCTTGAACCACTTCGTGTTATTCTCATGACTAAAGTAAATGAAACCGCTGT
TAAACAACAAATCGAACGTAATGAAGAATGTATTCGTTCAGCTTTACGTGCTGTCGCTTC
CATCTCTAGAATTCCAAATAGTGATAGTATCGTTAAATTTGAAGAATTTGTTAAAAATAC
AATTCGTACAACTCCATTAGCTGCTCAATTTAATTCAATTTTATC
Length of connected seq. 1235
Full length Seq ID -
Full length Seq. -
Length of full length seq. -