>VSA218 (VSA218Q) /CSM/VS/VSA2-A/VSA218Q.Seq.d/
XXXXXXXXXXAATGTTTTCGGTGTTATCTCTGTAATTCGTAATGCACTTCCACATTTACG
TATAAATAAGTTTTTTGCAAATGGGCCTAGAATAATTAACATTTCATCAATTGCTGGATT
TACAGGATCATTTCCAGGATTTAGTATTTATTCATCAACTAAATTCGCATTGGAAGGTTT
AACAGAAGGTTTGAATGAAGATTTGAAAGAGTTTGGTATACATGCATCAACTGTATTACC
AGGTTATTTTAGAACATCATTTTTAGAGAAAGGTTCAGTGGCAGTTCCATCACATCCAAT
TAAAGAATATACAGCAGTTAGAAAAATTCAAGAAATTCATGAAAATCAAATTAATGGTAA
TCAACAAGGTGATCCTGTAAAAGGTTGTAAAGTTATCATTGATCATGCATTATCTGAAAA
TCCAACAACTCATCTTTTCTTAGGTCCAGACTCAATGAAATATGCTCAATCCAAAATTGA
AGCAATTCAAAATGATATCAAATCAAATTATGATGCAGCTTCTAAAACTGATTTCACAAA
TTAATTTAATAAATAAAAATAAAAAAAAAAGTACAATTTACTATTTAA

---VFGVISVIRNALPHLRINKFFANGPRIINISSIAGFTGSFPGFSIYSSTKFALEGLT
EGLNEDLKEFGIHASTVLPGYFRTSFLEKGSVAVPSHPIKEYTAVRKIQEIHENQINGNQ
QGDPVKGCKVIIDHALSENPTTHLFLGPDSMKYAQSKIEAIQNDIKSNYDAASKTDFTN*
fnk*k*kkkynllf

Frame A:
---mfsvlsl*fvmhfhiyv*isflqmgle*ltfhqlldlqdhfqdlvfihqlnshwkv*
qkv*mki*kslvymhqlyyqvilehhf*rkvqwqfhhiqlkniqqlekfkkfmkiklmvi
nkvil*kvvklslimhylkiqqlifs*vqtq*nmlnpklkqfkmisnqimmqllklisqi
nlinknkkkstiyyl


Frame B:
---cfrcylcns*ctstftyk*vfckwa*nn*hfincwiyriisri*ylfin*irigrfn
rrfe*rfervwytcincitrlf*niifrerfsgssitsn*riyss*knsrns*ksn*w*s
tr*sckrl*syh*scii*ksnnssflrsrlneicsiqn*snsk*yqikl*csf*n*fhkl
i**ikikkkvqfti*


Frame C:
---VFGVISVIRNALPHLRINKFFANGPRIINISSIAGFTGSFPGFSIYSSTKFALEGLT
EGLNEDLKEFGIHASTVLPGYFRTSFLEKGSVAVPSHPIKEYTAVRKIQEIHENQINGNQ
QGDPVKGCKVIIDHALSENPTTHLFLGPDSMKYAQSKIEAIQNDIKSNYDAASKTDFTN*
fnk*k*kkkynllf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD394 (VSD394Q) /CSM/VS/VSD3-D/VSD394Q.Seq.d/                       1078   0.0
VSA218 (VSA218Q) /CSM/VS/VSA2-A/VSA218Q.Seq.d/                       1078   0.0
VFL636 (VFL636Q) /CSM/VF/VFL6-B/VFL636Q.Seq.d/                        963   0.0
VSG293 (VSG293Q) /CSM/VS/VSG2-D/VSG293Q.Seq.d/                        902   0.0
VSD393 (VSD393Q) /CSM/VS/VSD3-D/VSD393Q.Seq.d/                        557   e-158
VSG358 (VSG358Q) /CSM/VS/VSG3-C/VSG358Q.Seq.d/                        297   1e-79
VHI476 (VHI476Q) /CSM/VH/VHI4-D/VHI476Q.Seq.d/                         38   0.15
AHL743 (AHL743Q) /CSM/AH/AHL7-B/AHL743Q.Seq.d/                         38   0.15
AHG487 (AHG487Q) /CSM/AH/AHG4-D/AHG487Q.Seq.d/                         38   0.15
AFH687 (AFH687Q) /CSM/AF/AFH6-D/AFH687Q.Seq.d/                         38   0.15

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AP003620|AP003620.1 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 6, PAC clone:P0596H06, WORKING DRAFT SEQUENCE, 1 ordered pieces.    52  0.003  5
AP003949|AP003949.1 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 6, BAC clone:OJ1269_H07, WORKING DRAFT SEQUENCE, 1 ordered pieces.    52  0.015  2
BD193850|BD193850.1 Enterococcus faecialis polynucleotides and polypeptides.    46  0.49  1
AZ667982|AZ667982.1 ENTMW10TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42  0.50  2
BH162623|BH162623.1 ENTSU65TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42  0.52  2
AL162717|AL162717.10 Human DNA sequence from clone RP11-123H22 on chromosome  13q14.2-21.2.    38  0.66  3
AJ517070|AJ517070.1 Arabidopsis thaliana GSS, clone 22B/933.      36  1.7  2
AZ543111|AZ543111.1 ENTES02TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38  1.8  2
AZ534494|AZ534494.1 ENTBE91TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38  1.9  2
AC078981|AC078981.19 Homo sapiens 3 BAC RP11-666A9 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    44  1.9  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000685_1834(CP000685|pid:none) Flavobacterium johnsoniae UW101...   164   1e-39
AC117072_34(AC117072|pid:none) Dictyostelium discoideum chromoso...   138   1e-31
CP000480_1581(CP000480|pid:none) Mycobacterium smegmatis str. MC...   134   2e-30
CP000284_588(CP000284|pid:none) Methylobacillus flagellatus KT, ...   130   3e-29
AJ628955_39(AJ628955|pid:none) Streptomyces rimosus subsp. parom...   129   6e-29
CP001026_795(CP001026|pid:none) Burkholderia ambifaria MC40-6 ch...   127   2e-28
AP009387_68(AP009387|pid:none) Burkholderia multivorans ATCC 176...   119   8e-26
CP000494_1096(CP000494|pid:none) Bradyrhizobium sp. BTAi1, compl...   117   2e-25
CP000680_2191(CP000680|pid:none) Pseudomonas mendocina ymp, comp...   115   7e-25
CP000960_881(CP000960|pid:none) Burkholderia cenocepacia MC0-3 c...   113   3e-24

 psg: 0.65  gvh: 0.48  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.36  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: mitochondrial
	 32.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VSA218 is mit

>VSA218Z.Seq
----------AATGTTTTCGGTGTTATCTCTGTAATTCGTAATGCACTTCCACATTTACG
TATAAATAAGTTTTTTGCAAATGGGCCTAGAATAATTAACATTTCATCAATTGCTGGATT
TACAGGATCATTTCCAGGATTTAGTATTTATTCATCAACTAAATTCGCATTGGAAGGTTT
AACAGAAGGTTTGAATGAAGATTTGAAAGAGTTTGGTATACATGCATCAACTGTATTACC
AGGTTATTTTAGAACATCATTTTTAGAGAAAGGTTCAGTGGCAGTTCCATCACATCCAAT
TAAAGAATATACAGCAGTTAGAAAAATTCAAGAAATTCATGAAAATCAAATTAATGGTAA
TCAACAAGGTGATCCTGTAAAAGGTTGTAAAGTTATCATTGATCATGCATTATCTGAAAA
TCCAACAACTCATCTTTTCTTAGGTCCAGACTCAATGAAATATGCTCAATCCAAAATTGA
AGCAATTCAAAATGATATCAAATCAAATTATGATGCAGCTTCTAAAACTGATTTCACAAA
TTAATTTAATAAATAAAAATAAAAAAAAAAGTACAATTTACTATTTAA