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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSA227
Library VS
(Link to library)
Clone ID VSA227
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16500-1
Original site URL
Representative seq. ID VSA227E
(Link to Original site)
Representative DNA sequence 
>VSA227 (VSA227Q) /CSM/VS/VSA2-B/VSA227Q.Seq.d/
AAAATAAATAAATAATAATAATAATAAATTTTAAAATGACTGATTTGAAAATTACATTAG
TTAATGAAGATGGGGAATCAACAATAAGTGGTAAAGGTCATCCATTACCAGCTCCCCTAA
TATTTCCTCCAATATATTGTTTCTGTTTTATCCAATATAAAACTGAAGGTAAACTTTGGG
ACAAAAATGATTTTCAAATAAAATCAGGTAAAATTGAATTCGGCGGAGAAGAATATGATA
TTACTGAATCTAAAGGCACATGGAGTAAAGATGATGAAGAAAACCACATCAAAGTTAGTT
TACATCTAATTGTACCACCAAAAAAAATTTTCCAAAAAAATTTTTAGATCTCAAAAACAG
ATACAATGAAAAACAAAGAAATAAAAAATTTGCATTAATCTTATTTTCATCATA
sequence update 2000. 2.14
Translated Amino Acid sequence 
nk*IIIIINFKMTDLKITLVNEDGESTISGKGHPLPAPLIFPPIYCFCFIQYKTEGKLWD
KNDFQIKSGKIEFGGEEYDITESKGTWSKDDEENHIKVSLHLIVPPKKIFQKNF*isktd
tmknkeiknlh*syfhh
Translated Amino Acid sequence (All Frames) 
Frame A:
kink*****ilk*li*klh*lmkmgnqq*vvkvihyqlp*yflqyivsvlsniklkvnfg
tkmifk*nqvklnsaeknmillnlkahgvkmmkkttsklvyi*lyhqkkfskkifrsqkq
iq*ktkk*kicinlifii


Frame B:
k*innnnnkf*nd*fenyis**rwginnkw*rssitsspnissnilflfypi*n*r*tlg
qk*fsnkir*n*irrrri*yy*i*rhme*r**rkphqs*ftsncttkknfpkkfldlknr
ynekqrnkkfalilfss


Frame C:
nk*IIIIINFKMTDLKITLVNEDGESTISGKGHPLPAPLIFPPIYCFCFIQYKTEGKLWD
KNDFQIKSGKIEFGGEEYDITESKGTWSKDDEENHIKVSLHLIVPPKKIFQKNF*isktd
tmknkeiknlh*syfhh
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH317 (VSH317Q) /CSM/VS/VSH3-A/VSH317Q.Seq.d/                        559   e-158
VSA227 (VSA227Q) /CSM/VS/VSA2-B/VSA227Q.Seq.d/                        559   e-158
VHQ253 (VHQ253Q) /CSM/VH/VHQ2-C/VHQ253Q.Seq.d/                        559   e-158
VFK101 (VFK101Q) /CSM/VF/VFK1-A/VFK101Q.Seq.d/                        559   e-158
SSM842 (SSM842Q) /CSM/SS/SSM8-B/SSM842Q.Seq.d/                        559   e-158
SSL312 (SSL312Q) /CSM/SS/SSL3-A/SSL312Q.Seq.d/                        559   e-158
SSK747 (SSK747Q) /CSM/SS/SSK7-B/SSK747Q.Seq.d/                        559   e-158
SSJ339 (SSJ339Q) /CSM/SS/SSJ3-B/SSJ339Q.Seq.d/                        559   e-158
SSI182 (SSI182Q) /CSM/SS/SSI1-D/SSI182Q.Seq.d/                        559   e-158
SSG857 (SSG857Q) /CSM/SS/SSG8-C/SSG857Q.Seq.d/                        559   e-158
own update 2004. 6. 2
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M27588|M27588.1 Dictyostelium discoideum CsbA (csbA) and CsbB (csbB) genes, complete cds.   446  e-150  3
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.   347  e-147  4
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    46  0.059  9
AC133802|AC133802.1 Rattus norvegicus clone CH230-129F10, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    38  0.14  3
AC090825|AC090825.5 Homo sapiens chromosome 15, clone CTD-2054N24, complete sequence.    46  0.16  2
CC569901|CC569901.1 CH240_445E23.TARBAC13P2 CHORI-240 Bos taurus genomic clone CH240_445E23, genomic survey sequence.    46  0.34  1
AC117974|AC117974.6 Rattus norvegicus clone CH230-423J24, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    46  0.34  1
AG210174|AG210174.1 Oryza sativa (japonica cultivar-group) DNA, clone:NF0007_0_103_1A, 3' flanking sequence of Tos17 insertion in rice strain NF0007.    40  0.65  2
AG210176|AG210176.1 Oryza sativa (japonica cultivar-group) DNA, clone:NF0007_0_402_1A, 3' flanking sequence of Tos17 insertion in rice strain NF0007.    40  0.87  2
CC304000|CC304000.1 CH261-89M10_Sp6.1 CH261 Gallus gallus genomic clone CH261-89M10, DNA sequence.    44  1.3  1
dna update 2003. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P16643) RecName: Full=Glycoprotein 24B;          Short=gp24B; A...   221   8e-57
(P16642) RecName: Full=Glycoprotein 24A;          Short=gp24A; A...   181   7e-45
(Q558X5) RecName: Full=Contact site B protein C;  &AC117075_12(A...   122   4e-27
(Q556L8) RecName: Full=Uncharacterized protein csb family protei...    89   6e-17
(Q54EJ7) RecName: Full=Uncharacterized protein csb family protei...    75   7e-13
(Q55B72) RecName: Full=Uncharacterized protein csb family protei...    74   2e-12
(Q55BG7) RecName: Full=Uncharacterized protein csb family protei...    71   1e-11
AC117075_43(AC117075|pid:none) Dictyostelium discoideum chromoso...    39   0.074
AM910988_160(AM910988|pid:none) Plasmodium knowlesi strain H chr...    33   4.1
EU856396_6(EU856396|pid:none) Unionicola foili mitochondrion, co...    32   6.9
protein update 2009. 3.18
PSORT 

 psg: 0.66  gvh: 0.48  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: peroxisomal

>> prediction for VSA227 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID VSA227E
Full length Seq. 
>VSA227E.Seq
AAAATAAATAAATAATAATAATAATAAATTTTAAAATGACTGATTTGAAAATTACATTAG
TTAATGAAGATGGGGAATCAACAATAAGTGGTAAAGGTCATCCATTACCAGCTCCCCTAA
TATTTCCTCCAATATATTGTTTCTGTTTTATCCAATATAAAACTGAAGGTAAACTTTGGG
ACAAAAATGATTTTCAAATAAAATCAGGTAAAATTGAATTCGGCGGAGAAGAATATGATA
TTACTGAATCTAAAGGCACATGGAGTAAAGATGATGAAGAAAACCACATCAAAGTTAGTT
TACATCTAATTGTACCACCAAAAAAAATTTTCCAAAAAAATTTTTAGATCTCAAAAACAG
ATACAATGAAAAACAAAGAAATAAAAAATTTGCATTAATCTTATTTTCATCATA
Length of full length seq. 414