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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD388
Library VS
(Link to library)
Clone ID VSD388
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15502-1
Original site URL
Representative seq. ID VSD388E
(Link to Original site)
Representative DNA sequence 
>VSD388 (VSD388Q) /CSM/VS/VSD3-D/VSD388Q.Seq.d/
TCAGGATTTCAATGTTTGATAGATACTGATGAATTATCACATTCTTCAATCATTCTTCGT
AAATTATCTTGATAAATATCTGTAACATATTCATAATTTGATTCAAACATATCGAAACCA
TCATCATATATAAGACCATTAGCACTACCACTAACTTGACTACCAATATTTAATAAAGAA
TTAAATAATTAAATAAACAAATAAATAAATAAATAAATAAATAAAATGACAGGAACTAGA
ACTTTTTTTGTTGGTGGAAATCATAAAATGAATGGTAGTAAATCAATGTTAGAATCTTTA
AGTAAAGGAATGAATGAATCAGTTGAAAATAAAGAAAATGTTGATATTTTCATTGCACCA
TCATATCCATATTTAGAATTTTTATCAAATAAATTAGATAATAAAAAGTTTAAAGTTTGT
TCACAAAAATTGTTATAGTGTTGCCAAAGGTGCATTCACAGGTGAAATCAGTGCTAATAT
GTTGGTTGATTTAGGTATTCCATACGTTATCATTGGTCATAGTGAGAGAAGAAATGTATT
TAGCGAATCAAGTGAGTTAATTACAAAGAAAACAAAGTATGCAATTTCATTAGGTCTCAC
AGTTATCTTATGTTTAGGTGAACTTTTAGCAGACAGAAAATCAAATAACACTGAACACAT
CCTAAGTGAACAATTGAAAGATTTCGCTTCATTCACTCCAGAAGAATGGTCAAAGATCGT
AATTGCCTATGAACCAGTTTGGGCAATTGGTACTGGTGCCGTTGCAACTCCACAAGAAGC
TCAAGATACTCATGTTTTCATTCGTAAATGGATTAGTGAAAAGGTTTCTGAAGATGTTGC
AAAGAAAACCTCGATCATGTATGGTGGTTCTGTTAATGCTGACAATTGTCATAACNTTTC
AAAGCAATCTGATATCGATGGTTTCTTAGTTGGTGGTGCTTCCCTTGTTGCCAGTGATTT
CATTGCCATCATTAAATCTGCTGTTCCAAAGAAATATTGAATCTCAAATTTATAAAAATA
AAAATAAAAAATAAATAAAAATCTATAAGNATTATTTTAATCTATAA
sequence update 2001. 3.25
Translated Amino Acid sequence 
qdfnv**ilmnyhilqsffvnyldkyl*hihnliqtyrnhhhi*dh*hyh*ldyqylikn
*iik*tnk*ink*ik*qelelfllveiik*mvvnqc*nl*vke*mnqlkikkmlifslhh
hihi*nfyqin*IIKSLKFVHKNCYSVAKGAFTGEISANMLVDLGIPYVIIGHSERRNVF
SESSELITKKTKYAISLGLTVILCLGELLADRKSNNTEHILSEQLKDFASFTPEEWSKIV
IAYEPVWAIGTGAVATPQEAQDTHVFIRKWISEKVSEDVAKKTSIMYGGSVNADNCHNXS
KQSDIDGFLVGGASLVASDFIAIIKSAVPKKY*isnl*k*k*kinknl*xlf*si
Translated Amino Acid sequence (All Frames) 
Frame A:
sgfqclidtdelshssiilrkls**isvtys*fdsniskpssyirplalplt*lpifnke
lnn*ink*ink*inkmtgtrtffvggnhkmngsksmleslskgmnesvenkenvdifiap
sypyleflsnkldnkkfkvcsqkll*ccqrcihr*nqc*yvg*frysiryhws**ekkci
*rik*vnykenkvcnfirshsylmfr*tfsrqkik*h*thpk*tierfrfihsrrmvkdr
ncl*tslgnwywcrcnstrssryscfhs*md**kgf*rcckenldhvwwfc*c*qls*xf
kai*yrwflswwcfpccq*fhchh*iccskeilnlkfikikiknk*ksixiiliy


Frame B:
qdfnv**ilmnyhilqsffvnyldkyl*hihnliqtyrnhhhi*dh*hyh*ldyqylikn
*iik*tnk*ink*ik*qelelfllveiik*mvvnqc*nl*vke*mnqlkikkmlifslhh
hihi*nfyqin*IIKSLKFVHKNCYSVAKGAFTGEISANMLVDLGIPYVIIGHSERRNVF
SESSELITKKTKYAISLGLTVILCLGELLADRKSNNTEHILSEQLKDFASFTPEEWSKIV
IAYEPVWAIGTGAVATPQEAQDTHVFIRKWISEKVSEDVAKKTSIMYGGSVNADNCHNXS
KQSDIDGFLVGGASLVASDFIAIIKSAVPKKY*isnl*k*k*kinknl*xlf*si


Frame C:
rismfdry**iitffnhss*iilinicnifii*fkhietiiiyktistttnlttni**ri
k*lnkqink*ink*ndrn*nffcwwks*new**invrifk*rne*is*k*rkc*yfhcti
isifrifik*ir**kv*slftkivivlpkvhsqvksvlicwli*vfhtlslvivreemyl
anqvs*lqrkqsmqfh*vsqlsyv*vnf*qtenqitlnts*vnn*kislhslqkngqrs*
lpmnqfgqlvlvplqlhkklkilmfsfvnglvkrflkmlqrkprscmvvllmltivitfq
snlismvs*lvvlpllpvislpslnllfqrniesqiyknknkk*ikiykxyfnl*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD388 (VSD388Q) /CSM/VS/VSD3-D/VSD388Q.Seq.d/                       1509   0.0
SSK644 (SSK644Q) /CSM/SS/SSK6-B/SSK644Q.Seq.d/                       1493   0.0
VSI710 (VSI710Q) /CSM/VS/VSI7-A/VSI710Q.Seq.d/                       1465   0.0
VSG406 (VSG406Q) /CSM/VS/VSG4-A/VSG406Q.Seq.d/                        856   0.0
SSM191 (SSM191Q) /CSM/SS/SSM1-D/SSM191Q.Seq.d/                        728   0.0
SSI865 (SSI865Q) /CSM/SS/SSI8-C/SSI865Q.Seq.d/                        539   e-152
SSL279 (SSL279Q) /CSM/SS/SSL2-D/SSL279Q.Seq.d/                        504   e-141
VSI633 (VSI633Q) /CSM/VS/VSI6-B/VSI633Q.Seq.d/                        424   e-117
AFG581 (AFG581Q) /CSM/AF/AFG5-D/AFG581Q.Seq.d/                        268   2e-70
VSA293 (VSA293Q) /CSM/VS/VSA2-D/VSA293Q.Seq.d/                         38   0.27
own update 2003. 7. 5
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1156  0.0  8
AF124845|AF124845.1 Candida albicans triose phosphate isomerase (TPI1) gene, complete cds.    46  2e-07  3
E17071|E17071.1 Candida albicans mRNA for antigen peptide.        46  7e-07  2
E24635|E24635.1 Antigen protein and nucleic acid encoding the same.    46  7e-07  2
AJ390491|AJ390491.1 Candida albicans mRNA for triosephosphate isomerase (tpi1 gene).    46  7e-07  2
AF063107|AF063107.1 Achlya bisexualis triosephosphate isomerase + glyceraldehyde-3-phosphate dehydrogenase (TigA) mRNA, complete cds.    50  2e-05  2
BE512130|BE512130.1 946067B04.y1 946 - tassel primordium prepared by Schmidt lab Zea mays cDNA, mRNA sequence.    44  3e-05  2
BU499963|BU499963.1 946180H10.y1 946 - tassel primordium prepared by Schmidt lab Zea mays cDNA, mRNA sequence.    44  3e-05  2
BU037919|BU037919.1 946143C03.y1 946 - tassel primordium prepared by Schmidt lab Zea mays cDNA, mRNA sequence.    44  3e-05  2
CF012915|CF012915.1 QBK20d11.xg QBK Zea mays cDNA clone QBK20d11, mRNA sequence.    44  3e-05  2
dna update 2003. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869R8) RecName: Full=Triosephosphate isomerase;          Short...   378   e-138
FN357296_92(FN357296|pid:none) Schistosoma mansoni genome sequen...   211   4e-60
(P48501) RecName: Full=Triosephosphate isomerase;          Short...   211   5e-60
CP000601_176(CP000601|pid:none) Ostreococcus lucimarinus CCE9901...   206   1e-59
AX172665_1(AX172665|pid:none) Sequence 155 from Patent WO0144476.     208   2e-59
EU976195_1(EU976195|pid:none) Zea mays clone 687466 triosephosph...   208   5e-59
CR954209_8(CR954209|pid:none) Ostreococcus tauri strain OTTH0595...   207   7e-59
EF147662_1(EF147662|pid:none) Populus trichocarpa clone WS0124_D...   205   7e-59
BT078479_1(BT078479|pid:none) Lepeophtheirus salmonis Pacific fo...   212   1e-58
(Q9M4S8) RecName: Full=Triosephosphate isomerase, chloroplastic;...   199   1e-57
protein update 2009. 7.29
PSORT 

 psg: 0.69  gvh: 0.44  alm: 0.27  top: 0.57  tms: 0.20  mit: 0.26  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 24.0 %: plasma membrane
	 16.0 %: nuclear
	 12.0 %: vacuolar
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for VSD388 is end
5' end seq. ID VSD388F
5' end seq. 
>VSD388F.Seq
TCAGGATTTCAATGTTTGATAGATACTGATGAATTATCACATTCTTCAATCATTCTTCGT
AAATTATCTTGATAAATATCTGTAACATATTCATAATTTGATTCAAACATATCGAAACCA
TCATCATATATAAGACCATTAGCACTACCACTAACTTGACTACCAATATTTAATAAAGAA
TTAAATAATTAAATAAACAAATAAATAAATAAATAAATAAATAAAATGACAGGAACTAGA
ACTTTTTTTGTTGGTGGAAATCATAAAATGAATGGTAGTAAATCAATGTTAGAATCTTTA
AGTAAAGGAATGAATGAATCAGTTGAAAATAAAGAAAATGTTGATATTTTCATTGCACCA
TCATATCCATATTTAGAATTTTTATCAAATAAATTAGATAATAAAAAGTTTAAAGTTTGT
TCACAAAATTGTTATAGTGTTGCCAAAGGTGCATTCACAGGTGAAATCAGTGC-------
---
Length of 5' end seq. 473
3' end seq. ID VSD388Z
3' end seq. 
>VSD388Z.Seq
----------TTCACAAAAATTGTTATAGTGTTGCCAAAGGTGCATTCACAGGTGAAATC
AGTGCTAATATGTTGGTTGATTTAGGTATTCCATACGTTATCATTGGTCATAGTGAGAGA
AGAAATGTATTTAGCGAATCAAGTGAGTTAATTACAAAGAAAACAAAGTATGCAATTTCA
TTAGGTCTCACAGTTATCTTATGTTTAGGTGAACTTTTAGCAGACAGAAAATCAAATAAC
ACTGAACACATCCTAAGTGAACAATTGAAAGATTTCGCTTCATTCACTCCAGAAGAATGG
TCAAAGATCGTAATTGCCTATGAACCAGTTTGGGCAATTGGTACTGGTGCCGTTGCAACT
CCACAAGAAGCTCAAGATACTCATGTTTTCATTCGTAAATGGATTAGTGAAAAGGTTTCT
GAAGATGTTGCAAAGAAAACCTCGATCATGTATGGTGGTTCTGTTAATGCTGACAATTGT
CATAACNTTTCAAAGCAATCTGATATCGATGGTTTCTTAGTTGGTGGTGCTTCCCTTGTT
GCCAGTGATTTCATTGCCATCATTAAATCTGCTGTTCCAAAGAAATATTGAATCTCAAAT
TTATAAAAATAAAAATAAAAAATAAATAAAAATCTATAAGNATTATTTTAATCTATAA
Length of 3' end seq. 648
Connected seq. ID VSD388P
Connected seq. 
>VSD388P.Seq
TCAGGATTTCAATGTTTGATAGATACTGATGAATTATCACATTCTTCAATCATTCTTCGT
AAATTATCTTGATAAATATCTGTAACATATTCATAATTTGATTCAAACATATCGAAACCA
TCATCATATATAAGACCATTAGCACTACCACTAACTTGACTACCAATATTTAATAAAGAA
TTAAATAATTAAATAAACAAATAAATAAATAAATAAATAAATAAAATGACAGGAACTAGA
ACTTTTTTTGTTGGTGGAAATCATAAAATGAATGGTAGTAAATCAATGTTAGAATCTTTA
AGTAAAGGAATGAATGAATCAGTTGAAAATAAAGAAAATGTTGATATTTTCATTGCACCA
TCATATCCATATTTAGAATTTTTATCAAATAAATTAGATAATAAAAAGTTTAAAGTTTGT
TCACAAAATTGTTATAGTGTTGCCAAAGGTGCATTCACAGGTGAAATCAGTGC-------
---TTCACAAAAATTGTTATAGTGTTGCCAAAGGTGCATTCACAGGTGAAATCAGTGCTA
ATATGTTGGTTGATTTAGGTATTCCATACGTTATCATTGGTCATAGTGAGAGAAGAAATG
TATTTAGCGAATCAAGTGAGTTAATTACAAAGAAAACAAAGTATGCAATTTCATTAGGTC
TCACAGTTATCTTATGTTTAGGTGAACTTTTAGCAGACAGAAAATCAAATAACACTGAAC
ACATCCTAAGTGAACAATTGAAAGATTTCGCTTCATTCACTCCAGAAGAATGGTCAAAGA
TCGTAATTGCCTATGAACCAGTTTGGGCAATTGGTACTGGTGCCGTTGCAACTCCACAAG
AAGCTCAAGATACTCATGTTTTCATTCGTAAATGGATTAGTGAAAAGGTTTCTGAAGATG
TTGCAAAGAAAACCTCGATCATGTATGGTGGTTCTGTTAATGCTGACAATTGTCATAACN
TTTCAAAGCAATCTGATATCGATGGTTTCTTAGTTGGTGGTGCTTCCCTTGTTGCCAGTG
ATTTCATTGCCATCATTAAATCTGCTGTTCCAAAGAAATATTGAATCTCAAATTTATAAA
AATAAAAATAAAAAATAAATAAAAATCTATAAGNATTATTTTAATCTATAA
Length of connected seq. 1121
Full length Seq ID VSD388E
Full length Seq. 
>VSD388E.Seq
TCAGGATTTCAATGTTTGATAGATACTGATGAATTATCACATTCTTCAATCATTCTTCGT
AAATTATCTTGATAAATATCTGTAACATATTCATAATTTGATTCAAACATATCGAAACCA
TCATCATATATAAGACCATTAGCACTACCACTAACTTGACTACCAATATTTAATAAAGAA
TTAAATAATTAAATAAACAAATAAATAAATAAATAAATAAATAAAATGACAGGAACTAGA
ACTTTTTTTGTTGGTGGAAATCATAAAATGAATGGTAGTAAATCAATGTTAGAATCTTTA
AGTAAAGGAATGAATGAATCAGTTGAAAATAAAGAAAATGTTGATATTTTCATTGCACCA
TCATATCCATATTTAGAATTTTTATCAAATAAATTAGATAATAAAAAGTTTAAAGTTTGT
TCACAAAAATTGTTATAGTGTTGCCAAAGGTGCATTCACAGGTGAAATCAGTGCTAATAT
GTTGGTTGATTTAGGTATTCCATACGTTATCATTGGTCATAGTGAGAGAAGAAATGTATT
TAGCGAATCAAGTGAGTTAATTACAAAGAAAACAAAGTATGCAATTTCATTAGGTCTCAC
AGTTATCTTATGTTTAGGTGAACTTTTAGCAGACAGAAAATCAAATAACACTGAACACAT
CCTAAGTGAACAATTGAAAGATTTCGCTTCATTCACTCCAGAAGAATGGTCAAAGATCGT
AATTGCCTATGAACCAGTTTGGGCAATTGGTACTGGTGCCGTTGCAACTCCACAAGAAGC
TCAAGATACTCATGTTTTCATTCGTAAATGGATTAGTGAAAAGGTTTCTGAAGATGTTGC
AAAGAAAACCTCGATCATGTATGGTGGTTCTGTTAATGCTGACAATTGTCATAACNTTTC
AAAGCAATCTGATATCGATGGTTTCTTAGTTGGTGGTGCTTCCCTTGTTGCCAGTGATTT
CATTGCCATCATTAAATCTGCTGTTCCAAAGAAATATTGAATCTCAAATTTATAAAAATA
AAAATAAAAAATAAATAAAAATCTATAAGNATTATTTTAATCTATAA
Length of full length seq. 1067