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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSH485
Library VS
(Link to library)
Clone ID VSH485
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09803-1
Original site URL
Representative seq. ID VSH485P
(Link to Original site)
Representative DNA sequence 
>VSH485 (VSH485Q) /CSM/VS/VSH4-D/VSH485Q.Seq.d/
GTTTCAGGAAAGAAATTCCCAAAGAAATTCGATCTCGAACCAAAAACAAGAATTATGAGA
GTACAAAATCTTCATTTAGCTATGCTTTTCATTGATGAAGATTTAAAAATTAAAGTTCAA
GGTGTTGCTGCTGAAGAATTTGTTGATAATAATAAAAAGATGATTTTGGGTTTTTTATGG
ACATTATATCGTAAATATCGTATTTCAGTTATTAATGAAGGTGATAAATCATCAGAGGAA
GGTTTATTAGCTTGGTGTAAAAAGACCACAGAAGGTTATAATAATGTTAATATCACAGGC
TTTAAATCTAGTTTCCGTGATGGTCATGGTTTCTTGGCATTAGCACATAAATATGATCCA
ACCGTTTTCAAATATGATGAATATGAAGGTCATGATAATATTGCTCGTTTGAATGCTGCC
TTTGATTTCGCAGAGAAAGGATTAGGTATTCCAAAGCTTTTAGAAGCTGAATCCCTCTCC
XXXXXXXXXXTAGCCTCACTCGAAACAATCTCTCGAAGGTGAGAAACACTCTCAAGAGGA
ATTGGTCAAACAAAAGAAGGATCTCGAAGATGCTCTCAACAAAATCAGAGAACAAAATGA
TAATAGAAACTCTCGTATCACCGATCTCCAATCAAAGATTGACGATGCCCTTCGTGGTCT
CGATGATGAGAAATTGGCTAAACTCGATCTCGAATCTCGTCTTGCCAAATGTGAAAAAGA
TAAAGCTATCCTCGAATTAAAACTCGCTGAAATCCTCGACGAAAAAGATAGACTCGAAAA
GAAAATCGATGAAGATAAAAAGAGAGCTGAAGCTGAACGTTTGGGTCTCGGTTTAATTCG
TCAACATCTCGCTGCTCAATTCTCTGATATTCATAAATGGCAATCCTTTTTGGAACATCC
AGAAACTGTTCCATACACTGGTACCCCTGTCGATTTAGATTCTGAACTCTCAAGTCTTTC
CTTTGAAGAACAAGCAAAGAAATTAGCTTCAAAATTAGAATCTGAAAATATTTTAATNAA
AAATATCTTAAGGTAAAGAAGAAGCAAAAGCTGCTGCTAAAAAATAAATCTAAATAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
VSGKKFPKKFDLEPKTRIMRVQNLHLAMLFIDEDLKIKVQGVAAEEFVDNNKKMILGFLW
TLYRKYRISVINEGDKSSEEGLLAWCKKTTEGYNNVNITGFKSSFRDGHGFLALAHKYDP
TVFKYDEYEGHDNIARLNAAFDFAEKGLGIPKLLEAESLS---

---SLTRNNLSKVRNTLKRNWSNKRRISKMLSTKSENKMIIETLVSPISNQRLTMPFVVS
MMRNWLNSISNLVLPNVKKIKLSSN*nslksstkkidskrksmkikrelklnvwvsv*fv
nisllnslifingnpfwniqklfhtlvplsi*ilnsqvfplknkqrn*lqn*nlkif*xk
is*gkeeakaaakk*i*i
Translated Amino Acid sequence (All Frames) 
Frame A:
VSGKKFPKKFDLEPKTRIMRVQNLHLAMLFIDEDLKIKVQGVAAEEFVDNNKKMILGFLW
TLYRKYRISVINEGDKSSEEGLLAWCKKTTEGYNNVNITGFKSSFRDGHGFLALAHKYDP
TVFKYDEYEGHDNIARLNAAFDFAEKGLGIPKLLEAESLS---

---*phskqslegekhsqeelvkqkkdledalnkireqndnrnsritdlqskiddalrgl
ddeklakldlesrlakcekdkailelklaeildekdrlekkidedkkraeaerlglglir
qhlaaqfsdihkwqsflehpetvpytgtpvdldselsslsfeeqakklasklesenilxk
nilr*rrskscc*kinlnk

Frame B:
fqernsqrnsisnqkqel*eykifi*lcfslmki*klkfkvlllknlliiikr*fwvfyg
hyivnivfqllmkvinhqrkvy*lgvkrpqkviimlisqalnlvsvmvmvswh*hinmiq
pfsnmmnmkvmiillv*mlplisqrkd*vfqsf*klnps---

---SLTRNNLSKVRNTLKRNWSNKRRISKMLSTKSENKMIIETLVSPISNQRLTMPFVVS
MMRNWLNSISNLVLPNVKKIKLSSN*nslksstkkidskrksmkikrelklnvwvsv*fv
nisllnslifingnpfwniqklfhtlvplsi*ilnsqvfplknkqrn*lqn*nlkif*xk
is*gkeeakaaakk*i*i

Frame C:
frkeipkeirsrtknknyestkssfsyafh**rfkn*ssrccc*ric****kddfgffmd
iis*isyfsy**r**iirgrfislv*kdhrrl**c*yhrl*i*fp*wswflgist*i*sn
rfqi**i*rs**ycsfeccl*frreriryskafrs*ipl---

---asletisrr*etlsrgigqtkegsrrcsqqnqrtk***klsyhrspikd*rcpswsr
**eig*trsrisscqm*kr*sypriktr*nprrkr*trkenr*r*kes*s*tfgsrfnss
tsrcsil*ys*mailfgtsrncsihwypcrfrf*tlksfl*rtskeisfkiri*kyfnxk
ylkvkkkqklllknksk*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH485 (VSH485Q) /CSM/VS/VSH4-D/VSH485Q.Seq.d/                       2072   0.0
SSJ769 (SSJ769Q) /CSM/SS/SSJ7-C/SSJ769Q.Seq.d/                       1116   0.0
VFK648 (VFK648Q) /CSM/VF/VFK6-B/VFK648Q.Seq.d/                       1112   0.0
VSD547 (VSD547Q) /CSM/VS/VSD5-B/VSD547Q.Seq.d/                       1106   0.0
AFF119 (AFF119Q) /CSM/AF/AFF1-A/AFF119Q.Seq.d/                       1094   0.0
VSC851 (VSC851Q) /CSM/VS/VSC8-C/VSC851Q.Seq.d/                       1082   0.0
SSL735 (SSL735Q) /CSM/SS/SSL7-B/SSL735Q.Seq.d/                       1080   0.0
VFA317 (VFA317Q) /CSM/VF/VFA3-A/VFA317Q.Seq.d/                       1065   0.0
SFF534 (SFF534Q) /CSM/SF/SFF5-B/SFF534Q.Seq.d/                       1017   0.0
VFA379 (VFA379Q) /CSM/VF/VFA3-D/VFA379Q.Seq.d/                        940   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(L46371) Dictyostelium discoideum cortexillin II mRNA, compl...   952   0.0    4
(AC115581) Dictyostelium discoideum chromosome 2 map complem...   688   0.0    5
(BJ428721) Dictyostelium discoideum cDNA clone:ddv1b05, 3' e...   460   0.0    5
(AU267522) Dictyostelium discoideum vegetative cDNA clone:VS...   952   0.0    1
(BJ415401) Dictyostelium discoideum cDNA clone:ddv22o12, 5' ...   952   0.0    1
(BJ410805) Dictyostelium discoideum cDNA clone:ddv1n19, 5' e...   940   0.0    1
(AU267523) Dictyostelium discoideum vegetative cDNA clone:VS...   492   0.0    2
(BJ410557) Dictyostelium discoideum cDNA clone:ddv1b05, 5' e...   839   0.0    3
(C91173) Dictyostelium discoideum slug cDNA, clone SSJ769.        466   0.0    3
(BJ433629) Dictyostelium discoideum cDNA clone:ddv22o12, 3' ...   466   0.0    3
dna update 2009. 6.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y0T4) RecName: Full=Cortexillin-1; AltName: Full=Cortexillin ...   237   6e-61
L08068_1(L08068|pid:none) Physarum polycephalum actin-binding pr...   197   2e-52
A48403(A48403)alpha-actinin - Caenorhabditis elegans (fragment)       127   5e-28
T26147(T26147) hypothetical protein W04D2.1a - Caenorhabditis el...   127   5e-28
AK304554_1(AK304554|pid:none) Homo sapiens cDNA FLJ58087 complet...   124   4e-27
(Q9QXQ0) RecName: Full=Alpha-actinin-4; AltName: Full=Non-muscle...   124   4e-27
D89980_1(D89980|pid:none) Homo sapiens mRNA for alpha actinin 4,...   124   4e-27
U19893_1(U19893|pid:none) Rattus norvegicus alpha actinin mRNA, ...   124   4e-27
(Q5RCS6) RecName: Full=Alpha-actinin-4; AltName: Full=Non-muscle...   124   4e-27
(O43707) RecName: Full=Alpha-actinin-4; AltName: Full=Non-muscle...   124   4e-27
protein update 2009. 3.23
PSORT 

 psg: 0.56  gvh: 0.32  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for VSH485 is cyt
5' end seq. ID VSH485F
5' end seq. 
>VSH485F.Seq
GTTTCAGGAAAGAAATTCCCAAAGAAATTCGATCTCGAACCAAAAACAAGAATTATGAGA
GTACAAAATCTTCATTTAGCTATGCTTTTCATTGATGAAGATTTAAAAATTAAAGTTCAA
GGTGTTGCTGCTGAAGAATTTGTTGATAATAATAAAAAGATGATTTTGGGTTTTTTATGG
ACATTATATCGTAAATATCGTATTTCAGTTATTAATGAAGGTGATAAATCATCAGAGGAA
GGTTTATTAGCTTGGTGTAAAAAGACCACAGAAGGTTATAATAATGTTAATATCACAGGC
TTTAAATCTAGTTTCCGTGATGGTCATGGTTTCTTGGCATTAGCACATAAATATGATCCA
ACCGTTTTCAAATATGATGAATATGAAGGTCATGATAATATTGCTCGTTTGAATGCTGCC
TTTGATTTCGCAGAGAAAGGATTAGGTATTCCAAAGCTTTTAGAAGCTGAATCCCTCTCC
----------
Length of 5' end seq. 480
3' end seq. ID VSH485Z
3' end seq. 
>VSH485Z.Seq
----------TAGCCTCACTCGAAACAATCTCTCGAAGGTGAGAAACACTCTCAAGAGGA
ATTGGTCAAACAAAAGAAGGATCTCGAAGATGCTCTCAACAAAATCAGAGAACAAAATGA
TAATAGAAACTCTCGTATCACCGATCTCCAATCAAAGATTGACGATGCCCTTCGTGGTCT
CGATGATGAGAAATTGGCTAAACTCGATCTCGAATCTCGTCTTGCCAAATGTGAAAAAGA
TAAAGCTATCCTCGAATTAAAACTCGCTGAAATCCTCGACGAAAAAGATAGACTCGAAAA
GAAAATCGATGAAGATAAAAAGAGAGCTGAAGCTGAACGTTTGGGTCTCGGTTTAATTCG
TCAACATCTCGCTGCTCAATTCTCTGATATTCATAAATGGCAATCCTTTTTGGAACATCC
AGAAACTGTTCCATACACTGGTACCCCTGTCGATTTAGATTCTGAACTCTCAAGTCTTTC
CTTTGAAGAACAAGCAAAGAAATTAGCTTCAAAATTAGAATCTGAAAATATTTTAATNAA
AAATATCTTAAGGTAAAGAAGAAGCAAAAGCTGCTGCTAAAAAATAAATCTAAATAAA
Length of 3' end seq. 588
Connected seq. ID VSH485P
Connected seq. 
>VSH485P.Seq
GTTTCAGGAAAGAAATTCCCAAAGAAATTCGATCTCGAACCAAAAACAAGAATTATGAGA
GTACAAAATCTTCATTTAGCTATGCTTTTCATTGATGAAGATTTAAAAATTAAAGTTCAA
GGTGTTGCTGCTGAAGAATTTGTTGATAATAATAAAAAGATGATTTTGGGTTTTTTATGG
ACATTATATCGTAAATATCGTATTTCAGTTATTAATGAAGGTGATAAATCATCAGAGGAA
GGTTTATTAGCTTGGTGTAAAAAGACCACAGAAGGTTATAATAATGTTAATATCACAGGC
TTTAAATCTAGTTTCCGTGATGGTCATGGTTTCTTGGCATTAGCACATAAATATGATCCA
ACCGTTTTCAAATATGATGAATATGAAGGTCATGATAATATTGCTCGTTTGAATGCTGCC
TTTGATTTCGCAGAGAAAGGATTAGGTATTCCAAAGCTTTTAGAAGCTGAATCCCTCTCC
----------TAGCCTCACTCGAAACAATCTCTCGAAGGTGAGAAACACTCTCAAGAGGA
ATTGGTCAAACAAAAGAAGGATCTCGAAGATGCTCTCAACAAAATCAGAGAACAAAATGA
TAATAGAAACTCTCGTATCACCGATCTCCAATCAAAGATTGACGATGCCCTTCGTGGTCT
CGATGATGAGAAATTGGCTAAACTCGATCTCGAATCTCGTCTTGCCAAATGTGAAAAAGA
TAAAGCTATCCTCGAATTAAAACTCGCTGAAATCCTCGACGAAAAAGATAGACTCGAAAA
GAAAATCGATGAAGATAAAAAGAGAGCTGAAGCTGAACGTTTGGGTCTCGGTTTAATTCG
TCAACATCTCGCTGCTCAATTCTCTGATATTCATAAATGGCAATCCTTTTTGGAACATCC
AGAAACTGTTCCATACACTGGTACCCCTGTCGATTTAGATTCTGAACTCTCAAGTCTTTC
CTTTGAAGAACAAGCAAAGAAATTAGCTTCAAAATTAGAATCTGAAAATATTTTAATNAA
AAATATCTTAAGGTAAAGAAGAAGCAAAAGCTGCTGCTAAAAAATAAATCTAAATAAA
Length of connected seq. 1068
Full length Seq ID -
Full length Seq. -
Length of full length seq. -