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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSJ469
Library VS
(Link to library)
Clone ID VSJ469
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10683-1
Original site URL
Representative seq. ID VSJ469P
(Link to Original site)
Representative DNA sequence 
>VSJ469 (VSJ469Q) /CSM/VS/VSJ4-C/VSJ469Q.Seq.d/
AATTAGAATGGGTATTTTTTGGATGGATCATGATATTGTAACAAGAAATTTAGAGAAAGC
TCAATCATTAGTTGAAGAAGGTGGTGATTGGGATAGAAAGAATCGTTTGAAAACATATGA
AGCAGTTTATAAAATGTCCATTAGACAATTTAAAGAAGCCTCCGATCTCTACTTGGAAAC
TGTTGCAAGTTTCACTTCAACTGAATTTATCGATTATAGTAGATTCATTCAATATTTAAT
TTTCACTTCATTACTTCATTTGGATAGAGTTTCCTTAAAACAAAAAGTTATCGATTCACC
AGATGTACTCTCTGTTATTAATGACACTCCAAAACTTCXXXXXXXXXXCTGATTCAATTA
AAGGCGANAGATATTTAGCTGNACANTCACGNTTCTTTACTANANAAATNAGNATTTTGG
CTTANAATCNATTTTTAGAATNTTATTCATCTGTAAAATTAGNATCAATGNCAAATCAAN
TTGGTGNATCTTATGNTTTTATTGANANAGAATTATCACGTTTTGNAGCAGCAGGTCGTT
TAAATTGNAAAATTGATAAAGNTTCAGGNGTCATTGAAACNACCNGAAGTGATGCNAAGA
ATCATTTATACAAAACTACNCTTCAACAAGGTGANAACTTATTNAATAGAGNTCNAAAAT
TA
sequence update 2001. 3.22
Translated Amino Acid sequence 
IRMGIFWMDHDIVTRNLEKAQSLVEEGGDWDRKNRLKTYEAVYKMSIRQFKEASDLYLET
VASFTSTEFIDYSRFIQYLIFTSLLHLDRVSLKQKVIDSPDVLSVINDTPKL---

---DSIKGXRYLAXXSRFFTXXXXILAXNXFLEXYSSVKLXSMXNQXGXSYXFIXXELSR
FXAAGRLNXKIDKXSGVIETTXSDAKNHLYKTTLQQGXNLXNRXXKL
Translated Amino Acid sequence (All Frames) 
Frame A:
n*ngyfldgs*ycnkkfressiis*rrw*lg*kesfeni*ssl*nvh*ti*rslrsllgn
cckfhfn*iyrl**ihsifnfhfitsfg*sflktksyrftrctlcy**hskt---

---liqlkaxdi*lxxhxsllxkxxfwlxixf*nxihl*n*xqxqixlvxlmxllxxnyh
vlxqqvv*ixklikxqxslkxpevmxriiytklxfnkvxtyxiexxn

Frame B:
IRMGIFWMDHDIVTRNLEKAQSLVEEGGDWDRKNRLKTYEAVYKMSIRQFKEASDLYLET
VASFTSTEFIDYSRFIQYLIFTSLLHLDRVSLKQKVIDSPDVLSVINDTPKL---

---*fn*rrxifsxtxtxlyxxnxxfglxsifrxlfickixinxksxwxilxfy*xriit
fxssrsfklxn**xfrxh*nxxk*cxesfiqnyxstr*xlix*xski

Frame C:
lewvffgwimil*qei*rklnh*lkkvvigieriv*khmkqfikcpldnlkkppistwkl
lqvslqlnlsiivdsfni*fslhyfiwiefp*nkklsihqmyslllmtlqnf---

---DSIKGXRYLAXXSRFFTXXXXILAXNXFLEXYSSVKLXSMXNQXGXSYXFIXXELSR
FXAAGRLNXKIDKXSGVIETTXSDAKNHLYKTTLQQGXNLXNRXXKL
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ469 (VSJ469Q) /CSM/VS/VSJ4-C/VSJ469Q.Seq.d/                       1074   0.0
AFA582 (AFA582Q) /CSM/AF/AFA5-D/AFA582Q.Seq.d/                        470   e-131
SSM892 (SSM892Q) /CSM/SS/SSM8-D/SSM892Q.Seq.d/                        444   e-124
AHC896 (AHC896Q) /CSM/AH/AHC8-D/AHC896Q.Seq.d/                        383   e-105
VFA490 (VFA490Q) /CSM/VF/VFA4-D/VFA490Q.Seq.d/                        349   3e-95
AFK561 (AFK561Q) /CSM/AF/AFK5-C/AFK561Q.Seq.d/                        347   1e-94
AFF282 (AFF282Q) /CSM/AF/AFF2-D/AFF282Q.Seq.d/                        337   1e-91
AFM613 (AFM613Q) /CSM/AF/AFM6-A/AFM613Q.Seq.d/                        321   8e-87
AFJ152 (AFJ152Q) /CSM/AF/AFJ1-C/AFJ152Q.Seq.d/                        321   8e-87
AHB478 (AHB478Q) /CSM/AH/AHB4-D/AHB478Q.Seq.d/                        313   2e-84
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AG261377|AG261377.1 Lotus japonicus DNA, clone:LjT52o21_sfi.      52  9e-06  2
BM886078|BM886078.1 sam12a07.y1 Gm-c1063 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1063-5030 5' similar to TR:Q9SZX2 Q9SZX2 PUTATIVE PROTEASOME REGULATORY SUBUNIT. ;, mRNA sequence.    48  9e-06  2
BM086589|BM086589.1 sah30g02.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-3003 5' similar to TR:Q9SZX2 Q9SZX2 PUTATIVE PROTEASOME REGULATORY SUBUNIT. ;, mRNA sequence.    48  1e-05  2
BI643417|BI643417.1 RS1_B03 Sugar beet root cDNA library (subtracted) Beta vulgaris cDNA 5', mRNA sequence.    50  2e-04  2
BI643292|BI643292.1 RS3_C04 Sugar beet root cDNA library (subtracted) Beta vulgaris cDNA 5' similar to 26S proteasome regulatory particle-like (e-60), mRNA sequence.    50  2e-04  2
BI643279|BI643279.1 RS3_E12 Sugar beet root cDNA library (subtracted) Beta vulgaris cDNA 5' similar to 26S proteasome regulatory particle-like (e-60), mRNA sequence.    50  2e-04  2
BE330639|BE330639.1 so82d11.y1 Gm-c1040 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1040-2158 5' similar to TR:O65072 O65072 KIAA0107-LIKE PROTEIN ;, mRNA sequence.    40  0.002  2
BQ605169|BQ605169.1 ar05-a10 Artemia EII Stage, Stratagene (cat. 200450) Artemia franciscana cDNA clone ar05-a10 5' similar to 26S proteasome regulatory complex subunit p42A, mRNA sequence.    48  0.007  2
CA835497|CA835497.1 MCS037F12_160502 Ice plant Lambda Uni-Zap XR expression library, 5 days 0.5 M NaCl treatment, Crassulacean acid metabolism, phase I (2 AM). Mesembryanthemum crystallinum cDNA clone MCS037F12 5, mRNA sequence.    50  0.035  1
BF595394|BF595394.1 su65f10.y1 Gm-c1069 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1069-1820 5' similar to TR:Q9SZX2 Q9SZX2 PUTATIVE PROTEASOME REGULATORY SUBUNIT. ;, mRNA sequence.    48  0.14  1
dna update 2003. 7.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55C75) RecName: Full=26S proteasome non-ATPase regulatory subu...   224   7e-95
AL049657_3(AL049657|pid:none) Arabidopsis thaliana DNA chromosom...   129   2e-49
DQ975456_1(DQ975456|pid:none) Capsicum annuum 26S proteasome sub...   132   2e-49
(Q93Y35) RecName: Full=Probable 26S proteasome non-ATPase regula...   129   3e-49
BT052608_1(BT052608|pid:none) Medicago truncatula clone MTYFD_FE...   129   7e-49
BT041149_1(BT041149|pid:none) Zea mays full-length cDNA clone ZM...   132   2e-48
DQ630904_1(DQ630904|pid:none) Gryllus firmus putative accessory ...   132   5e-47
DQ630907_1(DQ630907|pid:none) Gryllus pennsylvanicus putative ac...   132   6e-47
BC064247_1(BC064247|pid:none) Xenopus tropicalis proteasome (pro...   137   2e-46
AF051233_1(AF051233|pid:none) Picea mariana KIAA0107-like protei...   119   2e-46
protein update 2009. 3.25
PSORT 

 psg: 0.75  gvh: 0.25  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VSJ469 is cyt
5' end seq. ID VSJ469F
5' end seq. 
>VSJ469F.Seq
AATTAGAATGGGTATTTTTTGGATGGATCATGATATTGTAACAAGAAATTTAGAGAAAGC
TCAATCATTAGTTGAAGAAGGTGGTGATTGGGATAGAAAGAATCGTTTGAAAACATATGA
AGCAGTTTATAAAATGTCCATTAGACAATTTAAAGAAGCCTCCGATCTCTACTTGGAAAC
TGTTGCAAGTTTCACTTCAACTGAATTTATCGATTATAGTAGATTCATTCAATATTTAAT
TTTCACTTCATTACTTCATTTGGATAGAGTTTCCTTAAAACAAAAAGTTATCGATTCACC
AGATGTACTCTCTGTTATTAATGACACTCCAAAACTTC----------
Length of 5' end seq. 338
3' end seq. ID VSJ469Z
3' end seq. 
>VSJ469Z.Seq
----------CTGATTCAATTAAAGGCGANAGATATTTAGCTGNACANTCACGNTTCTTT
ACTANANAAATNAGNATTTTGGCTTANAATCNATTTTTAGAATNTTATTCATCTGTAAAA
TTAGNATCAATGNCAAATCAANTTGGTGNATCTTATGNTTTTATTGANANAGAATTATCA
CGTTTTGNAGCAGCAGGTCGTTTAAATTGNAAAATTGATAAAGNTTCAGGNGTCATTGAA
ACNACCNGAAGTGATGCNAAGAATCATTTATACAAAACTACNCTTCAACAAGGTGANAAC
TTATTNAATAGAGNTCNAAAATTA
Length of 3' end seq. 314
Connected seq. ID VSJ469P
Connected seq. 
>VSJ469P.Seq
AATTAGAATGGGTATTTTTTGGATGGATCATGATATTGTAACAAGAAATTTAGAGAAAGC
TCAATCATTAGTTGAAGAAGGTGGTGATTGGGATAGAAAGAATCGTTTGAAAACATATGA
AGCAGTTTATAAAATGTCCATTAGACAATTTAAAGAAGCCTCCGATCTCTACTTGGAAAC
TGTTGCAAGTTTCACTTCAACTGAATTTATCGATTATAGTAGATTCATTCAATATTTAAT
TTTCACTTCATTACTTCATTTGGATAGAGTTTCCTTAAAACAAAAAGTTATCGATTCACC
AGATGTACTCTCTGTTATTAATGACACTCCAAAACTTC----------CTGATTCAATTA
AAGGCGANAGATATTTAGCTGNACANTCACGNTTCTTTACTANANAAATNAGNATTTTGG
CTTANAATCNATTTTTAGAATNTTATTCATCTGTAAAATTAGNATCAATGNCAAATCAAN
TTGGTGNATCTTATGNTTTTATTGANANAGAATTATCACGTTTTGNAGCAGCAGGTCGTT
TAAATTGNAAAATTGATAAAGNTTCAGGNGTCATTGAAACNACCNGAAGTGATGCNAAGA
ATCATTTATACAAAACTACNCTTCAACAAGGTGANAACTTATTNAATAGAGNTCNAAAAT
TA
Length of connected seq. 652
Full length Seq ID -
Full length Seq. -
Length of full length seq. -