| 2144 | |
| NO. | 2144 |
| ***** Sequence and structural information***** | - |
| PROTEIN | Ribonuclease HI |
| SOURCE | Escherichia coli |
| LENGTH | 155 |
| MOL-WEIGHT | 17740.11 |
| PIR_ID | NRECH |
| SWISSPROT_ID | RNH_ECOLI (P0A7Y4) |
| E.C.NUMBER | EC 3.1.4.8 |
| PMD.NO | A920875 |
| PDB_wild | 2RN2 Homologous PDB Entries |
| PDB_mutant | - |
| MUTATION | K 91 R, D 94 E |
| MUTATED_CHAIN | - |
| NO_MOLECULE | 1 |
| SEC.STR. | Coil, Coil |
| ASA | 80.5, 99.6 |
| ***** Experimental condition ***** | - |
| T | - |
| pH | 5.50 |
| BUFFER_NAME | glycine-HCl |
| BUFFER_CONC | 10 mM |
| ION_NAME_1 | - |
| ION_CONC_1 | - |
| PROTEIN_CONC | - |
| MEASURE | CD |
| METHOD | Thermal |
| ***** Thermodynamic data ***** | - |
| dG_H2O | - |
| ddG_H2O | - |
| dG | - |
| ddG | - |
| Tm | 50.2 |
| dTm | -1.8 |
| dHvH | 87.5 |
| dHcal | - |
| m | - |
| Cm | - |
| dCp | - |
| STATE | - |
| REVERSIBILITY | yes |
| ACTIVITY | 130 |
| ACTIVITY_Km | - |
| ACTIVITY_Kcat | - |
| ACTIVITY_Kd | - |
| ***** Literature ***** | - |
| KEY_WORDS | structural stability; mutagenesis; free energy change; thermostabilization; Escherichia coli ribonuclease HI |
| REFERENCE | J BIOL CHEM 267, 22014-22017 (1992) PMID: 1331044 |
| AUTHOR | Kimura S., Kanaya S. & Nakamura H. |
| REMARKS | ddG was measured at Tm (52.0) of wild type |
| RELATED_ENTRIES | 5,6,7,8,9,10,11,12,13,14,15,16,2143,2145,2146,2147,2148,13174,13175,13176,13177,13178,13179,13180,13181,13182,13183,13939,13940,13941,13942,13943,13944 |