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BRET experiments using chimeric V1a or V2 vasopressin or oxytocin and receptors labeled on the C-terminus with luciferase or yellow fluorescent protein led to specific BRET signals, suggesting the formation of V1a, V2 and OT homodimers. In addition, coexpressions of different pairs of receptors (V1a/V2, V1a/OTR and V2/OTR) also resulted in a specific BRET signal. BRET50 values (corresponding to the probability of one receptor to interact with another receptor) were identical whatever the pair of receptors coexpressed in the cells, which suggests that the proportion of homodimers and heterodimers depends on the relative expression of each receptor. |