160
GRIP ID 160
Main Title Information about hetero oligomer between opioid recetpro ( OPR) and CXCR2 chemokine receptor (CXCR2), and interfaces of OPR
GPCR Class A
GPCR Subfamily Opioid
GPCR Subtype delta
GPCR Name deltaOPR
Partner Class A
Partner Subfamily Chemokine
Partner Subtype CXCR2
Partner Name CXCR2
Evidence Experiment
Annotations Hetero-oligomerization of the human deltaOPR and CXCR2 could be detected by using coimmunoprecipitation, BRET, BRET50, BRETmax and the construction of pairs of individually inactive but potentially complementary receptor G-protein alpha subunit fusion proteins. CXCR2 antagonist enhances the function of deltaOPR agonists in the presence of CXCR2, which is observed for both enkephalin- and alkaloid-based opioid agonists, and the effective concentrations of the CXCR2 antagonist reflected CXCR2 occupancy. These results indicate that a CXCR antagonist can enhance the function of agonists at a receptor for which it has no inherent direct affinity by acting as an allosteric regulator of a receptor.
Articles 18307412
Methods coimmunoprecipitation,BRET,BRET50,BRETmax,construction of pairs of individually inactive but potentially complementary receptor G-protein alpha subunit fusion proteins
Target NP_000903.2
Mutation Information
Experimentaly Information
Predicted Information