| SE58 > MS01 | |
| ID | SE58_MS01 |
| Title | GC-TOF-MS |
| Instrument | Agilent 6890N gas chromatograph (Agilent Technologies) and Pegasus IV TOF mass spectrometer (LECO) |
| Instrument Type | - |
| Ionization Method | EI |
| Ion Mode | Positive |
| Description | For metabolome analysis, 1 μL of extracts (∼5.6 μg of each sample) was injected in the splitless mode by a CTC CombiPAL autosampler (CTC Analytics, Zwingen, Switzerland) into an Agilent 6890N gas chromatograph (Agilent Technologies, Wilmington, DE, USA) equipped with a 30 m × 0.25 mm inner diameter fused-silica capillary column with a chemically bound 0.25 μL film Rtx-5 Sil MS stationary phase (Restek, Bellefonte, PA, USA). Helium was used as the carrier gas at a constant flow rate of 1 mL min-1. The temperature program for metabolome analysis started with a 2 min isothermal step at 80 ℃ and this was followed by temperature ramping at 30 ℃ to a final temperature of 320 ℃, which was maintained for 3.5 min. The transfer line and the ion source temperatures were 250 and 200 ℃, respectively. Ions were generated by a 70 eV electron beam at an ionization current of 2.0 mA. The acceleration voltage was turned on after a solvent delay of 237 s. Data acquisition was performed on a Pegasus IV TOF mass spectrometer (LECO, St. Joseph, MI, USA) with an acquisition rate of 30 spectra s-1 in the mass rangeof a mass-to-charge ratio of 60 ≦ m/z ≦ 800. Alkane standard mixtures (C8-C20 and C21-C40) were purchased from Sigma-Aldrich (Tokyo, Japan) and were used for calculating the retention index (RI). The normalized response for the calculation of the signal intensity of each metabolite from the mass-detector response was obtained by each selected ion current that was unique in each metabolite MS spectrumto normalize the peak response. For quality control, we injected methylstearate into every sixth sample. |
| Comment_of_details | - |