| Preparation |
Solanum lycopersicum cv. Micro-Tom WT, self polinated over 8 generations is used in this study. Seeds were sawn in the pot (500 ml) filled with mixture of vermiculite and Powersoil (mix ratio 1 to 1, Kureha Chemical Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan) and kept in the controlled room at 25C. Until germination, seeds were covered with plastic film and kept dark. After 4 days of germination, they were grown with photoperiod of 16 hr light(7000 lux) / 8 hr dark. Hyponex(R) (Hyponex Ltd., Osaka, Japan), 1000 times diluted, was applied to plants once a week as a nutrient. Peel of fruit at the stage of breaker (approx. 35 days after anthesis) is harvested as sample "BP". |