SE58 |
Sample Set ID |
SE58 |
Title |
Exploring matrix effects and quantification performance in metabolomics experiments using artificial biological gradients |
Description |
We introduce a powerful approach that provides semiquantitative calibration curves over a biologically defined concentration range for all detected compounds. By performing metabolomics on a stepwise gradient between two biological specimens, we obtain a data set where each peak would ideally show a linear dependency on the mixture ratio. An example gradient between extracts of tomato leaf and fruit demonstrates good calibration statistics for a large proportion of the peaks but also highlights cases with strong background-dependent signal interference. Analysis of artificial biological gradients is a general and inexpensive tool for calibration that greatly facilitates data interpretation, quality control and method comparisons. |
Authors |
Henning Redestig, Makoto Kobayashi, Kazuki Saito, Miyako Kusano |
Reference |
Henning R et al. (2011) Analytical Chemistry 83: 5645-5651 |
Comment |
- |
Experimental Conditions |
conditions (1) |