| 1V02 | |
| PDB ID | 1V02 |
| Japanese name | ソルガム |
| English name | Sorghum |
| Scientific name | Sorghum bicolor Moench |
| Protein name | Dhurrinase. |
| Gene name | - |
| Source/Organism | Sorghum Bicolor |
| Compound | Molecule: Dhurrinase; Synonym: Dhurrinase-1; Chain: A, B, C, D, F; Engineered: Yes; Molecule: Dhurrinase; Synonym: Dhurrinase-1; Chain: E; Engineered: Yes |
| Classification | Hydrolase |
| EC number | 3.2.1.21 (Dhurrinase) |
| Authors | J.Moriniere, L.Verdoucq, D.R.Bevan, A.Esen, B.Henrissat, M.Czjzek |
| Reference | L.Verdoucq, J.Moriniere, D.R.Bevan, A.Esen, A.Vasella, B.Henrissat, M.Czjzek Structural Determinants Of Substrate Specificity In Family 1 Beta -Glucosidases: Novel Insights From The Crystal Structure Of Sorghum Dhurrinase-1, A Plant Beta -Glucosidase With Strict Specificity, In Complex With Its Natural Substrate To Be Published |
| Key word | Beta-Glucosidase, Dhurrin Hydrolysis, Pest Defense, Family Gh1, Hydrolase |
| Cross reference | UNP:Q41290,Q41290 EMBL; U33817; AAC49177.1; -. PIR; T14732; T14732. PDB; 1V02; X-ray; A/B/C/D/E/F=-. PDB; 1V03; X-ray; A=-. GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl ...; IEA. GO; GO:0005975; P:carbohydrate metabolism; IEA. InterPro; IPR001360; Glyco_hydro_1. Pfam; PF00232; Glyco_hydro_1; 1. PRINTS; PR00131; GLHYDRLASE1. PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; UNKNOWN_1. PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1. |
| Experimental method | X-Ray Diffraction, Resolution: 1.80 Angstrom, R-Factor: 0.17156, R-Free: 0.20974 |
| Sequence characteristics | Length: 565 AA, Molecular weight: 63779 Da |
| Sequence | MALLLASAINHTAHPAGLRSHPNNESFSRHHLCSSPQNISKRRSNLSFRPRAQTISSESA GIHRLSPWEIPRRDWFPPSFLFGAATSAYQIEGAWNEDGKGPSTWDHFCHNFPEWIVDRS NGDVAADSYHMYAEDVRLLKEMGMDAYRFSISWPRILPKGTLAGGINEKGVEYYNKLIDL LLENGIEPYITIFHWDTPQALVDAYGGFLDEEDYKDYTDFAKVCFEKFGKTVKNWLTFNE PETFCSVSYGTGVLAPGRCSPGVSCAVPTGNSLSEPYIVAHNLLRAHAETVDIYNKYHKG ADGRIGLALNVFGRVPYTNTFLDQQAQERSMDKCLGWFLEPVVRGDYPFSMRVSARDRVP YFKEKEQEKLVGSYDMIGINYYTSTFSKHIDLSPNNSPVLNTDDAYASQETKGPDGNAIG PPTGNAWINMYPKGLHDILMTMKNKYGNPPMYITENGMGDIDKGDLPKPVALEDHTRLDY IQRHLSVLKQSIDLGADVRGYFAWSLLDNFEWSSGYTERFGIVYVDRENGCERTMKRSAR WLQEFNGAAKKVENNKILTPAGQLN |
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