| 1H49 | |
| PDB ID | 1H49 |
| Japanese name | トウモロコシ |
| English name | Corn |
| Scientific name | Zea mays L. |
| Protein name | Beta-Glucosidase, Chloroplast Precursor |
| Gene name | Name=Glu1; |
| Source/Organism | Zea Mays |
| Compound | Molecule: Beta-Glucosidase; Synonym: Chloroplast Precursor, Gentiobiase, Cellobiase, Beta-D-Glucoside Glucohydrolase, Glu1; Chain: A, B; Fragment: Beta-Glucosidase, Residues 55-566; Engineered: Yes; Mutation: Yes; Other_details: In Complex With The Ligand Dimboa-Glucoside |
| Classification | Hydrolase |
| EC number | 3.2.1.21 (Beta-Glucosidase) |
| Authors | M.Czjzek, J.Moriniere, L.Verdoucq, D.R.Bevan, B.Henrissat, A.Esen |
| Reference | L.Verdoucq, M.Czjzek, J.Moriniere, D.R.Bevan, A.Esen Mutational And Structural Analysis Of Aglycone Specificity In Maize And Sorghum Beta-Glucosidases J.Biol.Chem. V. 278 25055 2003 |
| Key word | Glycoside Hydrolase, Beta-Glucosidase, Family 1, Retention Of The Anomeric Configuration, Inactive Mutant E191d |
| Cross reference | SWS:BGLC_MAIZE,P49235 EMBL; U25157; AAA65946.1; -. EMBL; X74217; CAA52293.1; -. PIR; A48860; A48860. PDB; 1E1E; X-ray; A/B=55-566. PDB; 1E1F; X-ray; A/B=55-566. PDB; 1E4L; X-ray; A/B=55-566. PDB; 1E4N; X-ray; A/B=55-566. PDB; 1E55; X-ray; A/B=55-566. PDB; 1E56; X-ray; A/B=55-566. PDB; 1H49; X-ray; A/B=55-566. PDB; 1HXJ; X-ray; A/B=60-566. Maize-2DPAGE; P49235; COLEOPTILE. MaizeDB; 13870; -. InterPro; IPR001360; Glyco_hydro_1. Pfam; PF00232; Glyco_hydro_1; 1. PRINTS; PR00131; GLHYDRLASE1. PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 1. PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1. |
| Experimental method | X-Ray Diffraction, Resolution: 1.90 Angstrom, R-Factor: 0.18353, R-Free: 0.22079 |
| Sequence characteristics | Length: 566 AA, Molecular weight: 64237 Da |
| Sequence | MAPLLAAAMNHAAAHPGLRSHLVGPNNESFSRHHLPSSSPQSSKRRCNLSFTTRSARVGS QNGVQMLSPSEIPQRDWFPSDFTFGAATSAYQIEGAWNEDGKGESNWDHFCHNHPERILD GSNSDIGANSYHMYKTDVRLLKEMGMDAYRFSISWPRILPKGTKEGGINPDGIKYYRNLI NLLLENGIEPYVTIFHWDVPQALEEKYGGFLDKSHKSIVEDYTYFAKVCFDNFGDKVKNW LTFNEPQTFTSFSYGTGVFAPGRCSPGLDCAYPTGNSLVEPYTAGHNILLAHAEAVDLYN KHYKRDDTRIGLAFDVMGRVPYGTSFLDKQAEERSWDINLGWFLEPVVRGDYPFSMRSLA RERLPFFKDEQKEKLAGSYNMLGLNYYTSRFSKNIDISPNYSPVLNTDDAYASQEVNGPD GKPIGPPMGNPWIYMYPEGLKDLLMIMKNKYGNPPIYITENGIGDVDTKETPLPMEAALN DYKRLDYIQRHIATLKESIDLGSNVQGYFAWSLLDNFEWFAGFTERYGIVYVDRNNNCTR YMKESAKWLKEFNTAKKPSKKILTPA |
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