| SKIP001150 | |
| SKIP ID | SKIP001150 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | frozen human embryo |
| Cell Tissue(Ja) | frozen human embryo |
| Cell Origin | Normal |
| Cell Name 1(En) | SEES5 |
| Cell Name 1(Ja) | SEES5 |
| Cell Name 2(En) | - |
| Cell Name 2(Ja) | - |
| Disease Name 1(Ja) | - |
| ICD Code 1 | - |
| Disease Name 1(En) | - |
| OMIM1 | - |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | - |
| Age Range | -- |
| Sex | Female |
| Race(En) | - |
| Race(Ja) | - |
| Genetic Diagnosis | -- |
| Not Detected | No |
| Description(En) | Human embryo-derived pluripotent embryonic stem (ES) cells.Under completely xenogeneic-free conditions. |
| Description(Ja) | 完全ゼノフリーのヒト胚由来の多能性胚性幹（ES）細胞 |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
| Vector | -- |
| Transgene | - |
| Adhesiveness | - |
| Feeder | Yes |
| Feeder Cell | inactivated human mesenchymal stem cell (hMSC) |
| Medium | 15% Knockout SR XenoFree CTS (KO-SR XF; Life Technologies; #12618-013), 85% KO-DMED, 2 mM GlutaMAX-I, 0.1 mM NEAAs, penicillin/streptomycin, 50 μg/mL l-ascorbic acid 2-phosphate (Sigma-Aldrich; #A4544), 10 ng/mL heregulin-1β, recombinant human NRG-beta 1/HRG-beta 1 EGF domain (R&D Systems, MN, USA; #396-HB-050/CF), 200 ng/mL LONG R3-IGF1, recombinant human insulin-like growth factor-1 (Sigma-Aldrich; #85580C), and recombinant human full-length bFGF (Life Technologies; #PHG0261) at 20 ng/mL. |
| Genome Editing | - |
| CO2 | 5% |
| Mycoplasma | - |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | Yes |
| Pluripotent Markers Assay | FACS, immunostaining |
| in vitro Differentiation | Yes |
| in vitro Differentiation Assay | EB formation |
| in vivo Differentiation | Yes |
| in vivo Differentiation Assay | teratoma formation |
| Other 1 Assay | Yes |
| Other 1 Assay Method | DMB-HPLC |
| Other 2 Assay | Yes |
| Other 2 Assay Method | ABO typing |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | Yes |
| Karyotype Assay | G-band |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | Yes |
| HLA | Yes |
| Stem Cell Transcriptome analysis | - |
| Stem Cell Transcriptome analysis Assay | - |
| Author Name(En) | Umezawa Akihiro |
| Author Name(Ja) | 梅澤 明弘 |
| Author Organization(En) | National Research Institute for Child Health and Development |
| Author Organization(Ja) | 国立成育医療研究センター |
| Author Contact Email | - |
| PI Organization(En) | National Research Institute for Child Health and Development |
| PI Organization(Ja) | 国立成育医療研究センター |
| PI Name(En) | Umezawa Akihiro |
| PI Name(Ja) | 梅澤 明弘 |
| PI Contact Email | - |
| Availability | Information Only |
| Provider Organization(En) | - |
| Provider Organization(Ja) | - |
| Provider Email | - |
| Provider URL | - |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | - |
| DOI | - |
| Title | Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells |
| Authors | Hidenori Akutsu,Masakazu Machida,Seiichi Kanzaki,Tohru Sugawara,Takashi Ohkura,Naoko Nakamura,Mayu Yamazaki-Inoue,Takumi Miura,Mohan C. Vemuri,Mahendra S. Rao,Kenji Miyado,Akihiro Umezawa |
| Journal | Regenerative Therapy |
| Year | 2015 |
| Volume | 1 |
| Issue | June |
| Pages | 18-29 |
| URL | http://dx.doi.org/10.1016/j.reth.2014.12.004 |
| Free input | - |
| Note | - |