SKIP000885 |
SKIP ID |
SKIP000885 |
Organism(En) |
the naked mole-rat/ Heterocephalus glaber |
Organism(Ja) |
ハダカデバネズミ |
Cell Type(En) |
- |
Cell Type(Ja) |
- |
Cell Tissue(En) |
fibroblasts |
Cell Tissue(Ja) |
線維芽細胞 |
Cell Origin |
Normal |
Cell Name 1(En) |
NMR-iPSCs clone 24 |
Cell Name 1(Ja) |
NMR-iPSCs clone 24 |
Cell Name 2(En) |
- |
Cell Name 2(Ja) |
- |
Disease Name 1(Ja) |
- |
ICD Code 1 |
- |
Disease Name 1(En) |
- |
OMIM1 |
- |
Disease Name 2(Ja) |
- |
ICD Code 2 |
- |
Disease Name 2(En) |
- |
OMIM 2 |
- |
Disease Name 3(Ja) |
- |
ICD Code 3 |
- |
Disease Name 3(En) |
- |
OMIM 3 |
- |
Age |
- |
Age Range |
0-9 |
Sex |
Male |
Race(En) |
the naked mole-rat/ Heterocephalus glaber |
Race(Ja) |
ハダカデバネズミ |
Genetic Diagnosis |
-- |
Not Detected |
No |
Description(En) |
NMR-iPSCs derive from the naked mole-rat (NMR, Heterocephalus glaber).NMR-iPSCs do not exhibit teratoma-forming tumorigenicity due to the species-specific activation of tumour-suppressor alternative reading frame (ARF) and a disruption mutation of the oncogene ES cell-expressed Ras (ERAS).INK4a was suppressed (as it is in both mice and humans) and ARF expression was retained in NMR-iPSCs. |
Description(Ja) |
がん化耐性ハダカデバネズミ由来iPS細胞株。 tumour-suppressor alternative reading frame (ARF)の活性化及び癌原遺伝子ERASの欠失により腫瘍を形成しない。qRT-PCRによりINK4aの発現は減少、ARFの発現は増加していることを確認。 |
Cell Morphology |
mouse ES-like |
Grade |
Research Grade |
Vector |
Retrovirus |
Transgene |
Oct4, Sox2, Klf4 and c-Myc |
Adhesiveness |
- |
Feeder |
Yes |
Feeder Cell |
mitomycin C-treated SNL-STO (MSTO) feeder feeder layers on gelatin-coated six-well plates |
Medium |
DMEM/F12 (Sigma Aldrich) supplemented with 20% knockout serum replacement (Life Technologies), 1% penicillin/streptomycin, 2 mM L-glutamine, 0.1 mM NEAA, 0.1 mM β-mercaptoethanol (2-ME, Sigma Aldrich) and 4 ng/ml fibroblast growth factor 2 (PeproTech). |
Genome Editing |
- |
CO2 |
- |
Mycoplasma |
- |
Detection of Contaminants Mycoplasma |
- |
Pluripotent Markers |
Yes |
Pluripotent Markers Assay |
RNA-seq |
in vitro Differentiation |
Yes |
in vitro Differentiation Assay |
Immunocytochemistry |
in vivo Differentiation |
Yes |
in vivo Differentiation Assay |
Teratomas forming assay |
Other 1 Assay |
- |
Other 1 Assay Method |
- |
Other 2 Assay |
- |
Other 2 Assay Method |
- |
Other 3 Assay |
- |
Other 3 Assay Method |
- |
Karyotype |
Yes |
Karyotype Assay |
G-band analyses |
Remaining Vector Detection |
- |
Remaining Vector Detection Assay |
- |
STR |
- |
HLA |
- |
Stem Cell Transcriptome analysis |
- |
Stem Cell Transcriptome analysis Assay |
- |
Author Name(En) |
Kyoko Miura |
Author Name(Ja) |
三浦恭子 |
Author Organization(En) |
Biomedical Animal Research Laboratory, Institute for Genetic Medicine, Hokkaido University, Hokkaido |
Author Organization(Ja) |
北海道大学 遺伝子病制御研究所 動物機能医科学研究室 |
Author Contact Email |
miura[at]igm[dot]hokudai[dot]ac[dot]jp |
PI Organization(En) |
- |
PI Organization(Ja) |
- |
PI Name(En) |
- |
PI Name(Ja) |
- |
PI Contact Email |
- |
Availability |
Information Only |
Provider Organization(En) |
Biomedical Animal Research Laboratory, Institute for Genetic Medicine, Hokkaido University, Hokkaido |
Provider Organization(Ja) |
北海道大学 遺伝子病制御研究所 動物機能医科学研究室 |
Provider Email |
miura[at]igm[dot]hokudai[dot]ac[dot]jp |
Provider URL |
http://www.igm.hokudai.ac.jp/debanezumi/ |
Ethical Statement(En) |
- |
Ethical Statement(Ja) |
- |
Terms of Use(En) |
- |
Terms of Use(Ja) |
- |
PubMed ID |
27161380 |
DOI |
10.1038/ncomms11471 |
Title |
Tumour resistance in induced pluripotent stem cells derived from naked mole-rats. |
Authors |
Shingo Miyawaki, Hideyuki Okano et al. |
Journal |
NATURE COMMUNICATIONS |
Year |
2015 |
Volume |
7 |
Issue |
11471 |
Pages |
- |
URL |
http://www.ncbi.nlm.nih.gov/pubmed/27161380 |
Free input |
- |
Note |
- |