| SKIP000188 | |
| SKIP ID | SKIP000188 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | - |
| Cell Tissue(Ja) | - |
| Cell Origin | Diseased |
| Cell Name 1(En) | R306C-iPS |
| Cell Name 1(Ja) | R306C-iPS |
| Cell Name 2(En) | GM23298 |
| Cell Name 2(Ja) | GM23298 |
| Disease Name 1(Ja) | レット症候群 |
| ICD Code 1 | F842 |
| Disease Name 1(En) | Rett syndrome |
| OMIM1 | 312750 |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | 8 |
| Age Range | 0-9 |
| Sex | Female |
| Race(En) | - |
| Race(Ja) | - |
| Genetic Diagnosis | Yes |
| Not Detected | No |
| Description(En) | Coriell ID:GM23298 iPSCs derived from GM11270 Fibroblast. Classical symptoms. MECP2 916C>T (R306C) mutation |
| Description(Ja) | Coriell ID:GM23298 レット症候群患者線維芽細胞（GM11270) 由来。典型的な症状。MECP2 遺伝子変異 916C>T (R306C)。 |
| Cell Morphology | human ES-like |
| Grade | -- |
| Vector | Retrovirus |
| Transgene | Oct4, Sox2, Klf, c-Myc, retroviral vector |
| Adhesiveness | Yes |
| Feeder | Yes |
| Feeder Cell | DR4 MEFs on 0.1% Gelatin |
| Medium | Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine |
| Genome Editing | - |
| CO2 | - |
| Mycoplasma | - |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | - |
| Pluripotent Markers Assay | - |
| in vitro Differentiation | - |
| in vitro Differentiation Assay | - |
| in vivo Differentiation | - |
| in vivo Differentiation Assay | - |
| Other 1 Assay | - |
| Other 1 Assay Method | - |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | Yes |
| Karyotype Assay | 46,XX[25] |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | - |
| HLA | - |
| Stem Cell Transcriptome analysis | - |
| Stem Cell Transcriptome analysis Assay | - |
| Author Name(En) | James Ellis |
| Author Name(Ja) | James Ellis |
| Author Organization(En) | Ontario Human iPS Facility, SickKids, University of Toronto |
| Author Organization(Ja) | Ontario Human iPS Facility, SickKids, University of Toronto |
| Author Contact Email | - |
| PI Organization(En) | - |
| PI Organization(Ja) | - |
| PI Name(En) | - |
| PI Name(Ja) | - |
| PI Contact Email | - |
| Availability | Available |
| Provider Organization(En) | NIGMS Coriell Cell Repository |
| Provider Organization(Ja) | NIGMS Coriell Cell Repository |
| Provider Email | - |
| Provider URL | http://ccr.coriell.org/Sections/Collections/NIGMS/ipsc_list.aspx?PgId=696 |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 19404254 |
| DOI | 10.1038/nmeth.1325 |
| Title | Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. |
| Authors | Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, and Ellis J. |
| Journal | Nat Methods |
| Year | 2009 |
| Volume | 6 |
| Issue | 5 |
| Pages | 370-6 |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/?term=19404254 |
| Free input | - |
| Note | - |