SKIP001133
SKIP ID SKIP001133
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Diseased→Genome editing
Cell Name 1(En) hiPS-RP#5Rw
Cell Name 1(Ja) hiPS-RP#5Rw
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) 網膜色素変性症
ICD Code 1 H35.5
Disease Name 1(En) retinitis pigmentosa
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range --
Sex Female
Race(En) -
Race(Ja) -
Genetic Diagnosis Yes
Not Detected No
Description(En) hiPSC, whose rhodopsin E181K mutation was corrected by knocking in a wildtype gene using helper-dependent adenoviral vector
Description(Ja) 網膜色素変性症患者繊維芽細胞由来iPS細胞。rhodopsin遺伝子E181K変異をゲノム編集によって健常に修復。
Cell Morphology human ES-like
Grade Research Grade
Vector Retrovirus
Transgene OCT4,SOX2,KLF4,C-MYC
Adhesiveness -
Feeder Yes
Feeder Cell MEF
Medium -
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers -
Pluripotent Markers Assay -
in vitro Differentiation -
in vitro Differentiation Assay -
in vivo Differentiation -
in vivo Differentiation Assay -
Other 1 Assay Yes
Other 1 Assay Method Differentiation of rod photoreceptor cells
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype -
Karyotype Assay -
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Yoko Ozawa
Author Name(Ja) 小沢洋子
Author Organization(En) Laboratory of Retinal Cell Biology, Department of Ophthalmology, Keio University School of Medicine
Author Organization(Ja) 慶應義塾大学医学部眼科学教室網膜細胞生物学研究室
Author Contact Email ozawa[at]a5[dot]keio[dot]jp
PI Organization(En) Laboratory of Retinal Cell Biology, Department of Ophthalmology, Keio University School of Medicine
PI Organization(Ja) 慶應義塾大学医学部眼科学教室網膜細胞生物学研究室
PI Name(En) Yoko Ozawa
PI Name(Ja) 小沢洋子
PI Contact Email ozawa[at]a5[dot]keio[dot]jp
Availability Available
Provider Organization(En) Laboratory of Retinal Cell Biology, Department of Ophthalmology, Keio University School of Medicine
Provider Organization(Ja) 慶應義塾大学医学部眼科学教室網膜細胞生物学研究室
Provider Email ozawa[at]a5[dot]keio[dot]jp
Provider URL http://www.keio-eye.net/
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 24935155
DOI 10.1186/1756-6606-7-45
Title The use of induced pluripotent stem cells to reveal pathogenic gene mutations and explore treatments for retinitis pigmentosa.
Authors Yoshida T, Ozawa Y, Suzuki K, Yuki K, Ohyama M, Akamatsu W, Matsuzaki Y, Shimmura S, Mitani K, Tsubota K, Okano H
Journal Mol Brain
Year 2014
Volume 7
Issue -
Pages 45
URL http://www.ncbi.nlm.nih.gov/pubmed/24935155
Free input -
Note -