SKIP001134 |
SKIP ID |
SKIP001134 |
Organism(En) |
- |
Organism(Ja) |
- |
Cell Type(En) |
- |
Cell Type(Ja) |
- |
Cell Tissue(En) |
- |
Cell Tissue(Ja) |
- |
Cell Origin |
Normal→Genome editing |
Cell Name 1(En) |
hiPS-201B7-Rm (Rhodopsin mutation E181K) |
Cell Name 1(Ja) |
hiPS-201B7-Rm (Rhodopsin mutation E181K) |
Cell Name 2(En) |
- |
Cell Name 2(Ja) |
- |
Disease Name 1(Ja) |
網膜色素変性症 |
ICD Code 1 |
H35.5 |
Disease Name 1(En) |
retinitis pigmentosa |
OMIM1 |
- |
Disease Name 2(Ja) |
- |
ICD Code 2 |
- |
Disease Name 2(En) |
- |
OMIM 2 |
- |
Disease Name 3(Ja) |
- |
ICD Code 3 |
- |
Disease Name 3(En) |
- |
OMIM 3 |
- |
Age |
- |
Age Range |
-- |
Sex |
-- |
Race(En) |
- |
Race(Ja) |
- |
Genetic Diagnosis |
Yes |
Not Detected |
No |
Description(En) |
hiPSC 201B7, whose rhodopsin E181K mutation was introduced by knocking in a gene with Rhodopsin E181K mutation using helper-dependent adenoviral vector |
Description(Ja) |
rhodopsin遺伝子E181K変異をゲノム編集によって導入した、健常人由来iPS細胞201B7株。 |
Cell Morphology |
human ES-like |
Grade |
Research Grade |
Vector |
Retrovirus |
Transgene |
OCT4,SOX2,KLF4,C-MYC |
Adhesiveness |
- |
Feeder |
Yes |
Feeder Cell |
MEF |
Medium |
- |
Genome Editing |
- |
CO2 |
- |
Mycoplasma |
- |
Detection of Contaminants Mycoplasma |
- |
Pluripotent Markers |
- |
Pluripotent Markers Assay |
- |
in vitro Differentiation |
- |
in vitro Differentiation Assay |
- |
in vivo Differentiation |
- |
in vivo Differentiation Assay |
- |
Other 1 Assay |
Yes |
Other 1 Assay Method |
Differentiation of rod photoreceptor cells |
Other 2 Assay |
- |
Other 2 Assay Method |
- |
Other 3 Assay |
- |
Other 3 Assay Method |
- |
Karyotype |
- |
Karyotype Assay |
- |
Remaining Vector Detection |
- |
Remaining Vector Detection Assay |
- |
STR |
- |
HLA |
- |
Stem Cell Transcriptome analysis |
- |
Stem Cell Transcriptome analysis Assay |
- |
Author Name(En) |
Yoko Ozawa |
Author Name(Ja) |
小沢洋子 |
Author Organization(En) |
Laboratory of Retinal Cell Biology, Department of Ophthalmology, Keio University School of Medicine |
Author Organization(Ja) |
慶應義塾大学医学部眼科学教室網膜細胞生物学研究室 |
Author Contact Email |
ozawa[at]a5[dot]keio[dot]jp |
PI Organization(En) |
Laboratory of Retinal Cell Biology, Department of Ophthalmology, Keio University School of Medicine |
PI Organization(Ja) |
慶應義塾大学医学部眼科学教室網膜細胞生物学研究室 |
PI Name(En) |
Yoko Ozawa |
PI Name(Ja) |
小沢洋子 |
PI Contact Email |
ozawa[at]a5[dot]keio[dot]jp |
Availability |
Available |
Provider Organization(En) |
Laboratory of Retinal Cell Biology, Department of Ophthalmology, Keio University School of Medicine |
Provider Organization(Ja) |
慶應義塾大学医学部眼科学教室網膜細胞生物学研究室 |
Provider Email |
ozawa[at]a5[dot]keio[dot]jp |
Provider URL |
http://www.keio-eye.net/ |
Ethical Statement(En) |
- |
Ethical Statement(Ja) |
- |
Terms of Use(En) |
- |
Terms of Use(Ja) |
- |
PubMed ID |
24935155 |
DOI |
10.1186/1756-6606-7-45 |
Title |
The use of induced pluripotent stem cells to reveal pathogenic gene mutations and explore treatments for retinitis pigmentosa. |
Authors |
Yoshida T, Ozawa Y, Suzuki K, Yuki K, Ohyama M, Akamatsu W, Matsuzaki Y, Shimmura S, Mitani K, Tsubota K, Okano H |
Journal |
Mol Brain |
Year |
2014 |
Volume |
7 |
Issue |
- |
Pages |
45 |
URL |
http://www.ncbi.nlm.nih.gov/pubmed/24935155 |
Free input |
- |
Note |
- |