SKIP001134
SKIP ID SKIP001134
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Normal→Genome editing
Cell Name 1(En) hiPS-201B7-Rm (Rhodopsin mutation E181K)
Cell Name 1(Ja) hiPS-201B7-Rm (Rhodopsin mutation E181K)
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) 網膜色素変性症
ICD Code 1 H35.5
Disease Name 1(En) retinitis pigmentosa
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range --
Sex --
Race(En) -
Race(Ja) -
Genetic Diagnosis Yes
Not Detected No
Description(En) hiPSC 201B7, whose rhodopsin E181K mutation was introduced by knocking in a gene with Rhodopsin E181K mutation using helper-dependent adenoviral vector
Description(Ja) rhodopsin遺伝子E181K変異をゲノム編集によって導入した、健常人由来iPS細胞201B7株。
Cell Morphology human ES-like
Grade Research Grade
Vector Retrovirus
Transgene OCT4,SOX2,KLF4,C-MYC
Adhesiveness -
Feeder Yes
Feeder Cell MEF
Medium -
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers -
Pluripotent Markers Assay -
in vitro Differentiation -
in vitro Differentiation Assay -
in vivo Differentiation -
in vivo Differentiation Assay -
Other 1 Assay Yes
Other 1 Assay Method Differentiation of rod photoreceptor cells
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype -
Karyotype Assay -
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Yoko Ozawa
Author Name(Ja) 小沢洋子
Author Organization(En) Laboratory of Retinal Cell Biology, Department of Ophthalmology, Keio University School of Medicine
Author Organization(Ja) 慶應義塾大学医学部眼科学教室網膜細胞生物学研究室
Author Contact Email ozawa[at]a5[dot]keio[dot]jp
PI Organization(En) Laboratory of Retinal Cell Biology, Department of Ophthalmology, Keio University School of Medicine
PI Organization(Ja) 慶應義塾大学医学部眼科学教室網膜細胞生物学研究室
PI Name(En) Yoko Ozawa
PI Name(Ja) 小沢洋子
PI Contact Email ozawa[at]a5[dot]keio[dot]jp
Availability Available
Provider Organization(En) Laboratory of Retinal Cell Biology, Department of Ophthalmology, Keio University School of Medicine
Provider Organization(Ja) 慶應義塾大学医学部眼科学教室網膜細胞生物学研究室
Provider Email ozawa[at]a5[dot]keio[dot]jp
Provider URL http://www.keio-eye.net/
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 24935155
DOI 10.1186/1756-6606-7-45
Title The use of induced pluripotent stem cells to reveal pathogenic gene mutations and explore treatments for retinitis pigmentosa.
Authors Yoshida T, Ozawa Y, Suzuki K, Yuki K, Ohyama M, Akamatsu W, Matsuzaki Y, Shimmura S, Mitani K, Tsubota K, Okano H
Journal Mol Brain
Year 2014
Volume 7
Issue -
Pages 45
URL http://www.ncbi.nlm.nih.gov/pubmed/24935155
Free input -
Note -