| SKIP000704 | |
| SKIP ID | SKIP000704 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | - |
| Cell Tissue(Ja) | - |
| Cell Origin | Diseased |
| Cell Name 1(En) | GM23298 |
| Cell Name 1(Ja) | GM23298 |
| Cell Name 2(En) | - |
| Cell Name 2(Ja) | - |
| Disease Name 1(Ja) | レット症候群 |
| ICD Code 1 | F842 |
| Disease Name 1(En) | RETT SYNDROME |
| OMIM1 | 312750 |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | - |
| Age Range | 0-9 |
| Sex | Female |
| Race(En) | - |
| Race(Ja) | - |
| Genetic Diagnosis | Yes |
| Not Detected | No |
| Description(En) | RETT SYNDROME hiPSC derived from fibroblast. Using the EOS (Early Transposon promoter and Oct-4 (Pou5f1) and Sox2 enhancers) lentiviral vector and puromycin selection, aid the isolation of iPS cell lines that expressed endogenous pluripotency markers. |
| Description(Ja) | ヒト線維芽細胞由来iPS細胞。 Early Transposon (ETn)由来のプロモーターに、Oct-4 (別名Pou5f1)のエンハンサー領域を三量化して連結することで、未分化な多能性幹細胞だけで特異的に発現するEOSレンチウイルスベクターとピューロマイシンでiPS細胞コロニーを選択。 |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
| Vector | Retrovirus |
| Transgene | Oct-4, Sox2, Klf4, c-Myc |
| Adhesiveness | Yes |
| Feeder | Yes |
| Feeder Cell | - |
| Medium | Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent + 20% Knock-out Serum Replacement Not inactivated |
| Genome Editing | - |
| CO2 | 5% |
| Mycoplasma | Negative |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | Yes |
| Pluripotent Markers Assay | Surface antigen expression and alkaline phosphatase |
| in vitro Differentiation | Yes |
| in vitro Differentiation Assay | EB formation assay |
| in vivo Differentiation | Yes |
| in vivo Differentiation Assay | Teratoma formation assay |
| Other 1 Assay | - |
| Other 1 Assay Method | - |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | Yes |
| Karyotype Assay | - |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | Yes |
| HLA | - |
| Stem Cell Transcriptome analysis | Yes |
| Stem Cell Transcriptome analysis Assay | Real-time PCR |
| Author Name(En) | Akitsu Hotta |
| Author Name(Ja) | 堀田秋津 |
| Author Organization(En) | Developmental and Stem Cell Biology Program and Ontario Human iPS Cell Facility |
| Author Organization(Ja) | Developmental and Stem Cell Biology Program and Ontario Human iPS Cell Facility |
| Author Contact Email | - |
| PI Organization(En) | Developmental and Stem Cell Biology Program and Ontario Human iPS Cell Facility |
| PI Organization(Ja) | Developmental and Stem Cell Biology Program and Ontario Human iPS Cell Facility |
| PI Name(En) | James Ellis |
| PI Name(Ja) | James Ellis |
| PI Contact Email | - |
| Availability | Available |
| Provider Organization(En) | Coriell Institute |
| Provider Organization(Ja) | Coriell Institute |
| Provider Email | - |
| Provider URL | https://catalog.coriell.org |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 19404254 |
| DOI | 10.1038/nmeth.1325 |
| Title | Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. |
| Authors | Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J |
| Journal | Nat Methods |
| Year | 2009 |
| Volume | 6 |
| Issue | 5 |
| Pages | 370-6 |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/19404254 |
| Free input | - |
| Note | - |