SKIP000737
SKIP ID SKIP000737
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Diseased
Cell Name 1(En) GM25267
Cell Name 1(Ja) GM25267
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) QT延長性症候群
ICD Code 1 I490
Disease Name 1(En) LONG QT SYNDROME 3
OMIM1 603830
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age 18
Age Range 10-19
Sex Female
Race(En) -
Race(Ja) -
Genetic Diagnosis Yes
Not Detected No
Description(En) LQT3 hiPSC derived from fibroblast
Description(Ja) LQT3患者線維芽細胞由来iPS細胞
Cell Morphology human ES-like
Grade Research Grade
Vector Retrovirus
Transgene KLF4, MYC, POU5F1, SOX2
Adhesiveness Yes
Feeder No
Feeder Cell -
Medium mTeSR1 + 20% Knock-out Serum Replacement
Genome Editing -
CO2 5%
Mycoplasma Negative
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay Surface antigen expression and alkaline phosphatase
in vitro Differentiation Yes
in vitro Differentiation Assay EB formation assay and Pluritest
in vivo Differentiation No
in vivo Differentiation Assay -
Other 1 Assay -
Other 1 Assay Method -
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay G-banding
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR Yes
HLA -
Stem Cell Transcriptome analysis Yes
Stem Cell Transcriptome analysis Assay Real-time PCR
Author Name(En) -
Author Name(Ja) -
Author Organization(En) -
Author Organization(Ja) -
Author Contact Email -
PI Organization(En) Gladstone Institute of Cardiovascular Disease, UCSF
PI Organization(Ja) Gladstone Institute of Cardiovascular Disease, UCSF
PI Name(En) Dr. Bruce R. Conklin
PI Name(Ja) Dr. Bruce R. Conklin
PI Contact Email -
Availability Available
Provider Organization(En) Coriell Institute
Provider Organization(Ja) Coriell Institute
Provider Email -
Provider URL https://catalog.coriell.org
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 24268663
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15840476
DOI 10.1016/j.biomaterials.2013.10.052
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10.1016/j.hrthm.2005.01.020
Title Three-dimensional filamentous human diseased cardiac tissue model.
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Compendium of cardiac channel mutations in 541 consecutive unrelated patients referred for long QT syndrome genetic testing.
Authors Ma Z, Koo S, Finnegan MA, Loskill P, Huebsch N, Marks NC, Conklin BR, Grigoropoulos CP, Healy KE
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Tester DJ, Will ML, Haglund CM, Ackerman MJ
Journal Biomaterials
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Heart Rhythm
Year 2013
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2005
Volume 35
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2
Issue 5
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5
Pages 1367-77
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507-17
URL http://www.ncbi.nlm.nih.gov/pubmed/24268663
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http://www.ncbi.nlm.nih.gov/pubmed/15840476
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