| SKIP000738 | |
| SKIP ID | SKIP000738 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | - |
| Cell Tissue(Ja) | - |
| Cell Origin | Diseased |
| Cell Name 1(En) | GM25305 |
| Cell Name 1(Ja) | GM25305 |
| Cell Name 2(En) | - |
| Cell Name 2(Ja) | - |
| Disease Name 1(Ja) | QT延長性症候群 |
| ICD Code 1 | I490 |
| Disease Name 1(En) | LONG QT SYNDROME 2 |
| OMIM1 | 613688 |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | 48 |
| Age Range | 40-49 |
| Sex | Female |
| Race(En) | Chinese |
| Race(Ja) | Chinese |
| Genetic Diagnosis | Yes |
| Not Detected | No |
| Description(En) | LONG QT SYNDROME 2 hiPSC derived from fibroblast |
| Description(Ja) | QT延長症候群患者線維芽細胞由来iPS細胞 |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
| Vector | Retrovirus |
| Transgene | KLF4, MYC, POU5F1, SOX2 |
| Adhesiveness | Yes |
| Feeder | No |
| Feeder Cell | - |
| Medium | mTeSR1 + 20% Knock-out Serum Replacement |
| Genome Editing | - |
| CO2 | 5% |
| Mycoplasma | - |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | Yes |
| Pluripotent Markers Assay | Surface antigen expression and alkaline phosphatase |
| in vitro Differentiation | Yes |
| in vitro Differentiation Assay | EB formation assay and Pluritest |
| in vivo Differentiation | Yes |
| in vivo Differentiation Assay | - |
| Other 1 Assay | - |
| Other 1 Assay Method | - |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | Yes |
| Karyotype Assay | G-banding |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | Yes |
| HLA | - |
| Stem Cell Transcriptome analysis | Yes |
| Stem Cell Transcriptome analysis Assay | Real-time PCR |
| Author Name(En) | C. Ian Spencer |
| Author Name(Ja) | C. Ian Spencer |
| Author Organization(En) | Gladstone Institute of Cardiovascular Disease |
| Author Organization(Ja) | Gladstone Institute of Cardiovascular Disease |
| Author Contact Email | - |
| PI Organization(En) | Gladstone Institute of Cardiovascular Disease |
| PI Organization(Ja) | Gladstone Institute of Cardiovascular Disease |
| PI Name(En) | Bruce R. Conklin |
| PI Name(Ja) | Bruce R. Conklin |
| PI Contact Email | - |
| Availability | Available |
| Provider Organization(En) | Coriell Institute |
| Provider Organization(Ja) | Coriell Institute |
| Provider Email | - |
| Provider URL | https://catalog.coriell.org |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 25254341 -- 15840476 |
| DOI | 10.1016/j.stemcr.2014.06.003 -- 10.1016/j.hrthm.2005.01.020 |
| Title | Calcium transients closely reflect prolonged action potentials in iPSC models of inherited cardiac arrhythmia. -- Compendium of cardiac channel mutations in 541 consecutive unrelated patients referred for long QT syndrome genetic testing. |
| Authors | Spencer CI, Baba S, Nakamura K, Hua EA, Sears MA, Fu CC, Zhang J, Balijepalli S, Tomoda K, Hayashi Y, Lizarraga P, Wojciak J, Scheinman MM, Aalto-Setaelae K, Makielski JC, January CT, Healy KE, Kamp TJ, Yamanaka S, Conklin BR -- Tester DJ, Will ML, Haglund CM, Ackerman MJ |
| Journal | Stem Cell Reports -- Heart Rhythm |
| Year | 2014 -- 2005 |
| Volume | 3 -- 2 |
| Issue | 2 -- 5 |
| Pages | 269-81 -- 507-17 |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/25254341 -- http://www.ncbi.nlm.nih.gov/pubmed/15840476 |
| Free input | -- |
| Note | - |