SKIP000218 |
SKIP ID |
SKIP000218 |
Organism(En) |
- |
Organism(Ja) |
- |
Cell Type(En) |
- |
Cell Type(Ja) |
- |
Cell Tissue(En) |
- |
Cell Tissue(Ja) |
- |
Cell Origin |
Normal |
Cell Name 1(En) |
409B2 |
Cell Name 1(Ja) |
409B2 |
Cell Name 2(En) |
HPS0076 |
Cell Name 2(Ja) |
HPS0076 |
Disease Name 1(Ja) |
- |
ICD Code 1 |
- |
Disease Name 1(En) |
- |
OMIM1 |
- |
Disease Name 2(Ja) |
- |
ICD Code 2 |
- |
Disease Name 2(En) |
- |
OMIM 2 |
- |
Disease Name 3(Ja) |
- |
ICD Code 3 |
- |
Disease Name 3(En) |
- |
OMIM 3 |
- |
Age |
36 |
Age Range |
30-39 |
Sex |
Female |
Race(En) |
- |
Race(Ja) |
- |
Genetic Diagnosis |
-- |
Not Detected |
No |
Description(En) |
Human iPS cell line, derived from same fibloblast as 201B7(HPS0063) and 253G1(HPS0002). |
Description(Ja) |
201B7(HPS0063)および253G1(HPS0002)と同一人由来。エピゾーマルベクターによる樹立、導入細胞には導入遺伝子は残っていない。 |
Cell Morphology |
human ES-like |
Grade |
-- |
Vector |
Plasmid |
Transgene |
Episomal vector pCXLE, Oct3/4, Sox2, Klf4, L-Myc, Lin28, p53 shRNA |
Adhesiveness |
- |
Feeder |
Yes |
Feeder Cell |
SNL 76/7 (X-rays:5000R or MMC) 1.5-2.5x10^(6) cells/100mm dish |
Medium |
Primate ES Cell Medium+4ng/mL human basic FGF |
Genome Editing |
- |
CO2 |
- |
Mycoplasma |
- |
Detection of Contaminants Mycoplasma |
- |
Pluripotent Markers |
- |
Pluripotent Markers Assay |
- |
in vitro Differentiation |
- |
in vitro Differentiation Assay |
- |
in vivo Differentiation |
- |
in vivo Differentiation Assay |
- |
Other 1 Assay |
- |
Other 1 Assay Method |
- |
Other 2 Assay |
- |
Other 2 Assay Method |
- |
Other 3 Assay |
- |
Other 3 Assay Method |
- |
Karyotype |
Yes |
Karyotype Assay |
46XX(20) |
Remaining Vector Detection |
- |
Remaining Vector Detection Assay |
- |
STR |
- |
HLA |
- |
Stem Cell Transcriptome analysis |
- |
Stem Cell Transcriptome analysis Assay |
- |
Author Name(En) |
Shinya Yamanaka |
Author Name(Ja) |
山中伸弥 |
Author Organization(En) |
Center for iPS Cell Research and Application, Kyoto University |
Author Organization(Ja) |
京都大学iPS細胞研究所 |
Author Contact Email |
- |
PI Organization(En) |
- |
PI Organization(Ja) |
- |
PI Name(En) |
- |
PI Name(Ja) |
- |
PI Contact Email |
- |
Availability |
Available |
Provider Organization(En) |
RIKEN BRC |
Provider Organization(Ja) |
理化学研究所バイオリソースセンター |
Provider Email |
- |
Provider URL |
http://www2.brc.riken.jp/lab/cell/detail.cgi?cell_no=HPS0076&type=1 |
Ethical Statement(En) |
- |
Ethical Statement(Ja) |
- |
Terms of Use(En) |
- |
Terms of Use(Ja) |
- |
PubMed ID |
21460823 |
DOI |
10.1038/nmeth.1591 |
Title |
A more efficient method to generate integration-free human iPS cells. |
Authors |
Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S |
Journal |
Nat Methods |
Year |
2011 |
Volume |
8 |
Issue |
5 |
Pages |
409-12 |
URL |
http://www.ncbi.nlm.nih.gov/pubmed/21460823 |
Free input |
- |
Note |
- |