SKIP000218
SKIP ID SKIP000218
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Normal
Cell Name 1(En) 409B2
Cell Name 1(Ja) 409B2
Cell Name 2(En) HPS0076
Cell Name 2(Ja) HPS0076
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age 36
Age Range 30-39
Sex Female
Race(En) -
Race(Ja) -
Genetic Diagnosis --
Not Detected No
Description(En) Human iPS cell line, derived from same fibloblast as 201B7(HPS0063) and 253G1(HPS0002).
Description(Ja) 201B7(HPS0063)および253G1(HPS0002)と同一人由来。エピゾーマルベクターによる樹立、導入細胞には導入遺伝子は残っていない。
Cell Morphology human ES-like
Grade --
Vector Plasmid
Transgene Episomal vector pCXLE, Oct3/4, Sox2, Klf4, L-Myc, Lin28, p53 shRNA
Adhesiveness -
Feeder Yes
Feeder Cell SNL 76/7 (X-rays:5000R or MMC) 1.5-2.5x10^(6) cells/100mm dish
Medium Primate ES Cell Medium+4ng/mL human basic FGF
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers -
Pluripotent Markers Assay -
in vitro Differentiation -
in vitro Differentiation Assay -
in vivo Differentiation -
in vivo Differentiation Assay -
Other 1 Assay -
Other 1 Assay Method -
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay 46XX(20)
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Shinya Yamanaka
Author Name(Ja) 山中伸弥
Author Organization(En) Center for iPS Cell Research and Application, Kyoto University
Author Organization(Ja) 京都大学iPS細胞研究所
Author Contact Email -
PI Organization(En) -
PI Organization(Ja) -
PI Name(En) -
PI Name(Ja) -
PI Contact Email -
Availability Available
Provider Organization(En) RIKEN BRC
Provider Organization(Ja) 理化学研究所バイオリソースセンター
Provider Email -
Provider URL http://www2.brc.riken.jp/lab/cell/detail.cgi?cell_no=HPS0076&type=1
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 21460823
DOI 10.1038/nmeth.1591
Title A more efficient method to generate integration-free human iPS cells.
Authors Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S
Journal Nat Methods
Year 2011
Volume 8
Issue 5
Pages 409-12
URL http://www.ncbi.nlm.nih.gov/pubmed/21460823
Free input -
Note -