SKIP000266
SKIP ID SKIP000266
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) Nasal Epithelial cells
Cell Tissue(Ja) 鼻腔上皮細胞
Cell Origin Normal
Cell Name 1(En) HPS0223
Cell Name 1(Ja) HPS0223
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age 43
Age Range 40-49
Sex Female
Race(En) -
Race(Ja) -
Genetic Diagnosis --
Not Detected No
Description(En) Human iPS cell line. In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS One. 2012;7:e42855.) designated by the DEPOSITOR is required.
Description(Ja) ヒト人工多能性幹(iPS)細胞株。
Cell Morphology --
Grade --
Vector Sendai virus
Transgene Oct3/4, sox2, Klf4, c-Myc,Sendai virus vector
Adhesiveness -
Feeder Yes
Feeder Cell MEF (X-rays:5000R or MMC) 1-1.5x10^(6) cells/100mm dish
Medium DMEM/HamF12(2mM L-Glutamine)+20%KSR+0.1mM NEAA+0.1mM 2-Mercaptoethanol+5ng/ml human bFGF
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers -
Pluripotent Markers Assay -
in vitro Differentiation -
in vitro Differentiation Assay -
in vivo Differentiation -
in vivo Differentiation Assay -
Other 1 Assay -
Other 1 Assay Method -
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype -
Karyotype Assay -
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Emiko Noguchi
Author Name(Ja) 野口恵美子
Author Organization(En) Graduate School of Comprehensive Human Sciences, University of Tsukuba
Author Organization(Ja) 国立大学法人 筑波大学大学院 人間総合科学研究科 遺伝医学
Author Contact Email -
PI Organization(En) Graduate School of Comprehensive Human Sciences, University of Tsukuba
PI Organization(Ja) 国立大学法人 筑波大学大学院 人間総合科学研究科 遺伝医学
PI Name(En) Emiko Noguchi
PI Name(Ja) 野口恵美子
PI Contact Email -
Availability Available
Provider Organization(En) Riken Bio Resource Center
Provider Organization(Ja) 理研BRC
Provider Email -
Provider URL http://www2.brc.riken.jp/lab/cell/detail.cgi?cell_no=HPS0223
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 22912751
DOI 10.1371/journal.pone.0042855
Title Generation of induced pluripotent stem cells from human nasal epithelial cells using a Sendai virus vector.
Authors Ono M, Hamada Y, Horiuchi Y, Matsuo-Takasaki M, Imoto Y, Satomi K, Arinami T, Hasegawa M, Fujioka T, Nakamura Y, Noguchi E
Journal PLoS One
Year 2012
Volume 7
Issue 8
Pages e42855
URL http://www.ncbi.nlm.nih.gov/pubmed/22912751
Free input -
Note 細胞名:NIps-B2
保存:DAP213 : Medium+2M DMSO+1M Acetamide+3M Propyleneglycol