SKIP000622 |
SKIP ID |
SKIP000622 |
Organism(En) |
- |
Organism(Ja) |
- |
Cell Type(En) |
- |
Cell Type(Ja) |
- |
Cell Tissue(En) |
- |
Cell Tissue(Ja) |
- |
Cell Origin |
Normal |
Cell Name 1(En) |
BJ hiPSC 5 factor #11 |
Cell Name 1(Ja) |
BJ hiPSC 5 factor #11 |
Cell Name 2(En) |
- |
Cell Name 2(Ja) |
- |
Disease Name 1(Ja) |
- |
ICD Code 1 |
- |
Disease Name 1(En) |
- |
OMIM1 |
- |
Disease Name 2(Ja) |
- |
ICD Code 2 |
- |
Disease Name 2(En) |
- |
OMIM 2 |
- |
Disease Name 3(Ja) |
- |
ICD Code 3 |
- |
Disease Name 3(En) |
- |
OMIM 3 |
- |
Age |
- |
Age Range |
0-9 |
Sex |
Male |
Race(En) |
- |
Race(Ja) |
- |
Genetic Diagnosis |
-- |
Not Detected |
No |
Description(En) |
hiPSCs from neonatal foreskin fibroblasts (BJ) with doxycycline (Dox)-inducibl lentiviruses containing the rtTA and either five factors(OCT4, SOX2, cMYC, KLF4,and NANOG) |
Description(Ja) |
新生児包皮繊維芽細胞に5因子を導入して作製したiPS細胞 |
Cell Morphology |
human ES-like |
Grade |
Research Grade |
Vector |
Lentivirus |
Transgene |
OCT4, SOX2, cMYC, KLF4 ,NANOG |
Adhesiveness |
- |
Feeder |
Yes |
Feeder Cell |
MEF |
Medium |
DMEM with 10% FBS, nonessential amino acids, glutamine, and beta-mercaptoethanol |
Genome Editing |
- |
CO2 |
- |
Mycoplasma |
- |
Detection of Contaminants Mycoplasma |
- |
Pluripotent Markers |
Yes |
Pluripotent Markers Assay |
- |
in vitro Differentiation |
Yes |
in vitro Differentiation Assay |
Immunofluorescence analysis |
in vivo Differentiation |
Yes |
in vivo Differentiation Assay |
teratoma formation assay |
Other 1 Assay |
Yes |
Other 1 Assay Method |
EB formation |
Other 2 Assay |
Yes |
Other 2 Assay Method |
Quantitative RT-PCR |
Other 3 Assay |
- |
Other 3 Assay Method |
- |
Karyotype |
Yes |
Karyotype Assay |
G-band |
Remaining Vector Detection |
- |
Remaining Vector Detection Assay |
- |
STR |
- |
HLA |
- |
Stem Cell Transcriptome analysis |
- |
Stem Cell Transcriptome analysis Assay |
- |
Author Name(En) |
Nimet Maherali |
Author Name(Ja) |
Nimet Maherali |
Author Organization(En) |
Massachusetts General Hospital Center |
Author Organization(Ja) |
Massachusetts General Hospital Center |
Author Contact Email |
- |
PI Organization(En) |
- |
PI Organization(Ja) |
- |
PI Name(En) |
- |
PI Name(Ja) |
- |
PI Contact Email |
- |
Availability |
Information Only |
Provider Organization(En) |
- |
Provider Organization(Ja) |
- |
Provider Email |
- |
Provider URL |
- |
Ethical Statement(En) |
- |
Ethical Statement(Ja) |
- |
Terms of Use(En) |
- |
Terms of Use(Ja) |
- |
PubMed ID |
18786420 |
DOI |
10.1016/j.stem.2008.08.003 |
Title |
A high-efficiency system for the generation and study of human induced pluripotent stem cells. |
Authors |
Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K |
Journal |
Cell Stem Cell |
Year |
2008 |
Volume |
3 |
Issue |
3 |
Pages |
340-5 |
URL |
http://www.ncbi.nlm.nih.gov/pubmed/18786420 |
Free input |
- |
Note |
- |