SKIP000622
SKIP ID SKIP000622
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Normal
Cell Name 1(En) BJ hiPSC 5 factor #11
Cell Name 1(Ja) BJ hiPSC 5 factor #11
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range 0-9
Sex Male
Race(En) -
Race(Ja) -
Genetic Diagnosis --
Not Detected No
Description(En) hiPSCs from neonatal foreskin fibroblasts (BJ) with doxycycline (Dox)-inducibl lentiviruses containing the rtTA and either five factors(OCT4, SOX2, cMYC, KLF4,and NANOG)
Description(Ja) 新生児包皮繊維芽細胞に5因子を導入して作製したiPS細胞
Cell Morphology human ES-like
Grade Research Grade
Vector Lentivirus
Transgene OCT4, SOX2, cMYC, KLF4 ,NANOG
Adhesiveness -
Feeder Yes
Feeder Cell MEF
Medium DMEM with 10% FBS, nonessential amino acids, glutamine, and beta-mercaptoethanol
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay -
in vitro Differentiation Yes
in vitro Differentiation Assay Immunofluorescence analysis
in vivo Differentiation Yes
in vivo Differentiation Assay teratoma formation assay
Other 1 Assay Yes
Other 1 Assay Method EB formation
Other 2 Assay Yes
Other 2 Assay Method Quantitative RT-PCR
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay G-band
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Nimet Maherali
Author Name(Ja) Nimet Maherali
Author Organization(En) Massachusetts General Hospital Center
Author Organization(Ja) Massachusetts General Hospital Center
Author Contact Email -
PI Organization(En) -
PI Organization(Ja) -
PI Name(En) -
PI Name(Ja) -
PI Contact Email -
Availability Information Only
Provider Organization(En) -
Provider Organization(Ja) -
Provider Email -
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 18786420
DOI 10.1016/j.stem.2008.08.003
Title A high-efficiency system for the generation and study of human induced pluripotent stem cells.
Authors Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K
Journal Cell Stem Cell
Year 2008
Volume 3
Issue 3
Pages 340-5
URL http://www.ncbi.nlm.nih.gov/pubmed/18786420
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