SKIP000309
SKIP ID SKIP000309
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Diseased
Cell Name 1(En) MJD1-9
Cell Name 1(Ja) MJD1-9
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) 脊髄小脳変性症3型/マシャド・ジョセフ病
ICD Code 1 G112
Disease Name 1(En) Spinocerebellar ataxia type 3 / Machado-Joseph disease
OMIM1 109150
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range 30-39
Sex Male
Race(En) -
Race(Ja) -
Genetic Diagnosis Yes
Not Detected No
Description(En) Human iPSC derived from SCA3 / MJD patient
Description(Ja) Human iPSC derived from SCA3 / MJD patient
Cell Morphology human ES-like
Grade Research Grade
Vector Retrovirus
Transgene OCT4, SOX2, KLF4, c-MYC
Adhesiveness -
Feeder Yes
Feeder Cell MEF
Medium DMEM/F12 culture medium supplemented with 20% KnockOut Serum Replacement, 0.1mM non-essential amino acids (all from Invitrogen), 1mM L-glutamine, 0.1mM beta-mercaptoethanol and 50 ng/ml zebrafish basic fibroblast growth factor (zbFGF)
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay ICC
in vitro Differentiation No
in vitro Differentiation Assay -
in vivo Differentiation Yes
in vivo Differentiation Assay teratoma formation
Other 1 Assay -
Other 1 Assay Method -
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay SNP analysis
Remaining Vector Detection No
Remaining Vector Detection Assay -
STR No
HLA No
Stem Cell Transcriptome analysis No
Stem Cell Transcriptome analysis Assay -
Author Name(En) Oliver Brustle
Author Name(Ja) Oliver Brustle
Author Organization(En) University of Bonn and Hertie Foundation
Author Organization(Ja) University of Bonn and Hertie Foundation
Author Contact Email brustle[at]uni-bonn[dot]de
PI Organization(En) -
PI Organization(Ja) -
PI Name(En) -
PI Name(Ja) -
PI Contact Email -
Availability Information Only
Provider Organization(En) University of Bonn and Hertie Foundation
Provider Organization(Ja) University of Bonn and Hertie Foundation
Provider Email -
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 22113611
DOI 10.1038/nature10671
Title Excitation-induced ataxin-3 aggregation in neurons from patients with Machado-Joseph disease.
Authors Koch P, Breuer P, Peitz M, Jungverdorben J, Kesavan J, Poppe D, Doerr J, Ladewig J, Mertens J, Tuting T, Hoffmann P, Klockgether T, Evert BO, Wullner U, Brustle O
Journal Nature
Year 2011
Volume 480
Issue 7378
Pages 543-6
URL http://www.ncbi.nlm.nih.gov/pubmed/22113611
Free input -
Note -