SKIP000291
SKIP ID SKIP000291
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Normal
Cell Name 1(En) C4
Cell Name 1(Ja) C4
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range --
Sex Female
Race(En) -
Race(Ja) -
Genetic Diagnosis --
Not Detected No
Description(En) Healthy control line for LRRK2 patient #2. Isogenic parent line of C4+G2019S, Subclone of C4 with inserted G2019S mutation.
Description(Ja) Healthy control line for LRRK2 patient #2. Isogenic parent line of C4+G2019S, Subclone of C4 with inserted G2019S mutation.
Cell Morphology --
Grade Research Grade
Vector Retrovirus
Transgene OSKM
Adhesiveness -
Feeder Yes
Feeder Cell MEF
Medium -
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay Immunostaining, qRT-PCR
in vitro Differentiation Yes
in vitro Differentiation Assay EB-mediated differentiation
in vivo Differentiation Yes
in vivo Differentiation Assay Teratoma formation
Other 1 Assay Yes
Other 1 Assay Method differentiation into midbrain dopaminergic neuron (Immunostaining, qRT-PCR)
Other 2 Assay Yes
Other 2 Assay Method Function of iPS cell derived neuron (Neurite outgrowth, 6-Hydroxydopamine sensitivity, Rotenone sensitivity)
Other 3 Assay Yes
Other 3 Assay Method microarray profiling of single-nucleotide polymorphisms
Karyotype Yes
Karyotype Assay qRT-PCR (silencing of the retroviral vectors)
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Thomas Gasser
Author Name(Ja) Thomas Gasser
Author Organization(En) Department of Neurodegenerative Diseases Hertie Institute for Clinical Brain Research, University of Tubingen, and German Center for Neurodegenerative Diseases
Author Organization(Ja) Department of Neurodegenerative Diseases Hertie Institute for Clinical Brain Research, University of Tubingen, and German Center for Neurodegenerative Diseases
Author Contact Email thomas[dot]gasser[at]uni-tuebingen[dot]de
PI Organization(En) -
PI Organization(Ja) -
PI Name(En) -
PI Name(Ja) -
PI Contact Email -
Availability Information Only
Provider Organization(En) -
Provider Organization(Ja) -
Provider Email -
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 23472874
DOI 10.1016/j.stem.2013.01.008
Title Genetic correction of a LRRK2 mutation in human iPSCs links parkinsonian neurodegeneration to ERK-dependent changes in gene expression.
Authors Reinhardt P, Schmid B, Burbulla LF, Schondorf DC, Wagner L, Glatza M, Hoing S, Hargus G, Heck SA, Dhingra A, Wu G, Muller S, Brockmann K, Kluba T, Maisel M, Kruger R, Berg D, Tsytsyura Y, Thiel CS, Psathaki OE, Klingauf J, Kuhlmann T, Klewin M, Muller H, Gasser T, Scholer HR, Sterneckert J
Journal Cell Stem Cell
Year 2013
Volume 12
Issue 3
Pages 354-67
URL http://www.ncbi.nlm.nih.gov/pubmed/23472874
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