SKIP000300
SKIP ID SKIP000300
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Diseased
Cell Name 1(En) L2-1GC
Cell Name 1(Ja) L2-1GC
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) パーキンソン病
ICD Code 1 G20
Disease Name 1(En) Parkinson's disease
OMIM1 607060
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range --
Sex Female
Race(En) -
Race(Ja) -
Genetic Diagnosis Yes
Not Detected No
Description(En) Gene corrected subclone of L2-1Mut (iPSC with LRRK2 mutation)
Description(Ja) Gene corrected subclone of L2-1Mut (iPSC with LRRK2 mutation)
Cell Morphology --
Grade Research Grade
Vector Retrovirus
Transgene OSKM
Adhesiveness -
Feeder --
Feeder Cell -
Medium -
Genome Editing LRRK2 G2019S\WT correction ZFN
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay Immunostaining, qRT-PCR
in vitro Differentiation Yes
in vitro Differentiation Assay EB-mediated differentiation
in vivo Differentiation Yes
in vivo Differentiation Assay Teratoma formation
Other 1 Assay Yes
Other 1 Assay Method differentiation into midbrain dopaminergic neuron (Immunostaining, qRT-PCR)
Other 2 Assay Yes
Other 2 Assay Method Function of iPS cell derived neuron (6-Hydroxydopamine sensitivity, Rotenone sensitivity)
Other 3 Assay Yes
Other 3 Assay Method Expression of mRNA and protein
Karyotype Yes
Karyotype Assay microarray profiling of single-nucleotide polymorphisms
Remaining Vector Detection Yes
Remaining Vector Detection Assay qRT-PCR (silencing of the retroviral vectors)
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Thomas Gasser
Author Name(Ja) Thomas Gasser
Author Organization(En) Department of Neurodegenerative Diseases Hertie Institute for Clinical Brain Research, University of Tubingen, and German Center for Neurodegenerative Diseases
Author Organization(Ja) Department of Neurodegenerative Diseases Hertie Institute for Clinical Brain Research, University of Tubingen, and German Center for Neurodegenerative Diseases
Author Contact Email thomas[dot]gasser[at]uni-tuebingen[dot]de
PI Organization(En) -
PI Organization(Ja) -
PI Name(En) -
PI Name(Ja) -
PI Contact Email -
Availability Information Only
Provider Organization(En) -
Provider Organization(Ja) -
Provider Email -
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 23472874
DOI 10.1016/j.stem.2013.01.008
Title Genetic correction of a LRRK2 mutation in human iPSCs links parkinsonian neurodegeneration to ERK-dependent changes in gene expression.
Authors Reinhardt P, Schmid B, Burbulla LF, Schondorf DC, Wagner L, Glatza M, Hoing S, Hargus G, Heck SA, Dhingra A, Wu G, Muller S, Brockmann K, Kluba T, Maisel M, Kruger R, Berg D, Tsytsyura Y, Thiel CS, Psathaki OE, Klingauf J, Kuhlmann T, Klewin M, Muller H, Gasser T, Schoer HR, Sterneckert J
Journal Cell Stem Cell
Year 2013
Volume 12
Issue 3
Pages 354-67
URL http://www.ncbi.nlm.nih.gov/pubmed/23472874
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