| SKIP000716 | |
| SKIP ID | SKIP000716 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | - |
| Cell Tissue(Ja) | - |
| Cell Origin | Normal |
| Cell Name 1(En) | UE7T-9 |
| Cell Name 1(Ja) | UE7T-9 |
| Cell Name 2(En) | - |
| Cell Name 2(Ja) | - |
| Disease Name 1(Ja) | - |
| ICD Code 1 | - |
| Disease Name 1(En) | - |
| OMIM1 | - |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | - |
| Age Range | -- |
| Sex | Female |
| Race(En) | - |
| Race(Ja) | - |
| Genetic Diagnosis | -- |
| Not Detected | No |
| Description(En) | Human bone marrow-derived mesenchymal stem cells which were infected with recombinant retroviruses expressing the E7 and hTERT. |
| Description(Ja) | 骨髄由来間葉系幹細胞, hTERT, HPV E7導入, 不死化細胞株 |
| Cell Morphology | fibroblast-like |
| Grade | -- |
| Vector | Retrovirus |
| Transgene | recombinant retroviruses expressing the E7 and hTERT. |
| Adhesiveness | Yes |
| Feeder | -- |
| Feeder Cell | - |
| Medium | poweredby10. When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells. |
| Genome Editing | - |
| CO2 | 5% |
| Mycoplasma | - |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | - |
| Pluripotent Markers Assay | - |
| in vitro Differentiation | - |
| in vitro Differentiation Assay | - |
| in vivo Differentiation | - |
| in vivo Differentiation Assay | - |
| Other 1 Assay | - |
| Other 1 Assay Method | - |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | - |
| Karyotype Assay | - |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | - |
| HLA | - |
| Stem Cell Transcriptome analysis | - |
| Stem Cell Transcriptome analysis Assay | - |
| Author Name(En) | Umezawa, Akihiro |
| Author Name(Ja) | 梅澤 明弘 |
| Author Organization(En) | National Center for Child Health and Development |
| Author Organization(Ja) | 国立成育医療研究センター研究所 |
| Author Contact Email | - |
| PI Organization(En) | - |
| PI Organization(Ja) | - |
| PI Name(En) | - |
| PI Name(Ja) | - |
| PI Contact Email | - |
| Availability | Not Available |
| Provider Organization(En) | National Institute of Biomedical Innovation. |
| Provider Organization(Ja) | 独立行政法人医薬基盤研究所ＪＣＲＢ細胞バンク |
| Provider Email | - |
| Provider URL | http://cellbank.nibio.go.jp/~cellbank/cgi-bin/search_res_det.cgi?DB_NUM=1&ID=3821 |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 15923633 |
| DOI | 10.1128/MCB.25.12.5183-5195.2005 |
| Title | Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential. |
| Authors | Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A |
| Journal | Mol Cell Biol |
| Year | 2005 |
| Volume | 25 |
| Issue | 12 |
| Pages | 5183-95 |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/15923633 |
| Free input | Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?Takeda Y,Mori T,Imabayashi H,Kiyono T,Gojo S,Miyoshi S,Hida N,Ita M,Segawa K,Ogawa S,Sakamoto M,Nakamura S,Umezawa A.,J Gene Med. 2004 Aug;6(8):833-45;PMID:15293342 |
| Note | - |