SKIP000396 |
SKIP ID |
SKIP000396 |
Organism(En) |
- |
Organism(Ja) |
- |
Cell Type(En) |
- |
Cell Type(Ja) |
- |
Cell Tissue(En) |
- |
Cell Tissue(Ja) |
- |
Cell Origin |
Normal |
Cell Name 1(En) |
GM23279 |
Cell Name 1(Ja) |
GM23279 |
Cell Name 2(En) |
- |
Cell Name 2(Ja) |
- |
Disease Name 1(Ja) |
- |
ICD Code 1 |
- |
Disease Name 1(En) |
- |
OMIM1 |
- |
Disease Name 2(Ja) |
- |
ICD Code 2 |
- |
Disease Name 2(En) |
- |
OMIM 2 |
- |
Disease Name 3(Ja) |
- |
ICD Code 3 |
- |
Disease Name 3(En) |
- |
OMIM 3 |
- |
Age |
36 |
Age Range |
30-39 |
Sex |
Female |
Race(En) |
- |
Race(Ja) |
- |
Genetic Diagnosis |
-- |
Not Detected |
No |
Description(En) |
Apparently healthy iPSCs induced from facial dermal fibroblasts |
Description(Ja) |
健常人皮膚線維芽細胞由来iPS細胞株 |
Cell Morphology |
human ES-like |
Grade |
Research Grade |
Vector |
Retrovirus |
Transgene |
OCT3/4, SOX2, Klf4, c-Myc |
Adhesiveness |
- |
Feeder |
Yes |
Feeder Cell |
CF1 MEFs on 0.1% Gelatin |
Medium |
DMEM/F12 + 20% KOSR + 10ng/ml bFGF |
Genome Editing |
- |
CO2 |
- |
Mycoplasma |
- |
Detection of Contaminants Mycoplasma |
- |
Pluripotent Markers |
Yes |
Pluripotent Markers Assay |
Immunostaining |
in vitro Differentiation |
Yes |
in vitro Differentiation Assay |
Embryoid Body Formation Assay, Differentiation (cardiac, pancreatic and neuronal) |
in vivo Differentiation |
Yes |
in vivo Differentiation Assay |
Teratoma Formation |
Other 1 Assay |
Yes |
Other 1 Assay Method |
Post-Thaw Viable Cell Recovery |
Other 2 Assay |
Yes |
Other 2 Assay Method |
Growth on agar |
Other 3 Assay |
Yes |
Other 3 Assay Method |
PCR Mycoplasma test |
Karyotype |
Yes |
Karyotype Assay |
G-banding |
Remaining Vector Detection |
- |
Remaining Vector Detection Assay |
- |
STR |
Yes |
HLA |
- |
Stem Cell Transcriptome analysis |
- |
Stem Cell Transcriptome analysis Assay |
- |
Author Name(En) |
Takahashi K |
Author Name(Ja) |
Takahashi K |
Author Organization(En) |
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University. |
Author Organization(Ja) |
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University. |
Author Contact Email |
- |
PI Organization(En) |
NIGMS Human Cell Repository |
PI Organization(Ja) |
NIGMS Human Cell Repository |
PI Name(En) |
NIGMS Human Genetic Cell Repository |
PI Name(Ja) |
NIGMS Human Genetic Cell Repository |
PI Contact Email |
- |
Availability |
Available |
Provider Organization(En) |
NIGMS Human Cell Repository |
Provider Organization(Ja) |
NIGMS Human Cell Repository |
Provider Email |
- |
Provider URL |
http://ccr.coriell.org/Sections/Search/Sample_Detail.aspx?PgId=166&Ref=GM23279 |
Ethical Statement(En) |
- |
Ethical Statement(Ja) |
- |
Terms of Use(En) |
- |
Terms of Use(Ja) |
- |
PubMed ID |
18035408 |
DOI |
10.1016/j.cell.2007.11.019 |
Title |
Induction of pluripotent stem cells from adult human fibroblasts by defined factors. |
Authors |
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S |
Journal |
Cell |
Year |
2007 |
Volume |
131 |
Issue |
5 |
Pages |
861-72 |
URL |
http://www.ncbi.nlm.nih.gov/pubmed/18035408 |
Free input |
- |
Note |
- |