SKIP000396
SKIP ID SKIP000396
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Normal
Cell Name 1(En) GM23279
Cell Name 1(Ja) GM23279
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age 36
Age Range 30-39
Sex Female
Race(En) -
Race(Ja) -
Genetic Diagnosis --
Not Detected No
Description(En) Apparently healthy iPSCs induced from facial dermal fibroblasts
Description(Ja) 健常人皮膚線維芽細胞由来iPS細胞株
Cell Morphology human ES-like
Grade Research Grade
Vector Retrovirus
Transgene OCT3/4, SOX2, Klf4, c-Myc
Adhesiveness -
Feeder Yes
Feeder Cell CF1 MEFs on 0.1% Gelatin
Medium DMEM/F12 + 20% KOSR + 10ng/ml bFGF
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay Immunostaining
in vitro Differentiation Yes
in vitro Differentiation Assay Embryoid Body Formation Assay, Differentiation (cardiac, pancreatic and neuronal)
in vivo Differentiation Yes
in vivo Differentiation Assay Teratoma Formation
Other 1 Assay Yes
Other 1 Assay Method Post-Thaw Viable Cell Recovery
Other 2 Assay Yes
Other 2 Assay Method Growth on agar
Other 3 Assay Yes
Other 3 Assay Method PCR Mycoplasma test
Karyotype Yes
Karyotype Assay G-banding
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR Yes
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Takahashi K
Author Name(Ja) Takahashi K
Author Organization(En) Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University.
Author Organization(Ja) Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University.
Author Contact Email -
PI Organization(En) NIGMS Human Cell Repository
PI Organization(Ja) NIGMS Human Cell Repository
PI Name(En) NIGMS Human Genetic Cell Repository
PI Name(Ja) NIGMS Human Genetic Cell Repository
PI Contact Email -
Availability Available
Provider Organization(En) NIGMS Human Cell Repository
Provider Organization(Ja) NIGMS Human Cell Repository
Provider Email -
Provider URL http://ccr.coriell.org/Sections/Search/Sample_Detail.aspx?PgId=166&Ref=GM23279
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 18035408
DOI 10.1016/j.cell.2007.11.019
Title Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
Authors Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S
Journal Cell
Year 2007
Volume 131
Issue 5
Pages 861-72
URL http://www.ncbi.nlm.nih.gov/pubmed/18035408
Free input -
Note -