SKIP000942
SKIP ID SKIP000942
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) dermal fibroblast
Cell Tissue(Ja) 表皮繊維芽細胞
Cell Origin Diseased
Cell Name 1(En) SP08.1
Cell Name 1(Ja) SP08.1
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) パーキンソン病
ICD Code 1 G20
Disease Name 1(En) Parkinson disease
OMIM1 168006
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age 66
Age Range 60-69
Sex Female
Race(En) -
Race(Ja) -
Genetic Diagnosis No
Not Detected No
Description(En) iPS cell line
Description(Ja) iPS細胞
Cell Morphology human ES-like
Grade Research Grade
Vector Retrovirus
Transgene SOX2, KLF4 and OCT3/4
Adhesiveness Yes
Feeder Yes
Feeder Cell MEFs
Medium Lines of patient-specific iPS cells were maintained by mechanical dissociation of colonies and splitting 1:3 onto feeder cells in hESC medium or by limited trypsin digestion and passaging onto Matrigel-coated plates with hESC medium pre-conditioned by mouse embryonic fibroblasts.
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay immunocytochemistry
in vitro Differentiation Yes
in vitro Differentiation Assay differentiation to three germ layers
in vivo Differentiation Yes
in vivo Differentiation Assay teratoma formation
Other 1 Assay -
Other 1 Assay Method -
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay -
Remaining Vector Detection Yes
Remaining Vector Detection Assay qRT-PCR
STR -
HLA -
Stem Cell Transcriptome analysis Yes
Stem Cell Transcriptome analysis Assay qRT-PCR
Author Name(En) Adriana Sanchez-Danes
Author Name(Ja) Adriana Sanchez-Danes
Author Organization(En) Institute for Biomedicine (IBUB), University of Barcelona,
Author Organization(Ja) Institute for Biomedicine (IBUB), University of Barcelona,
Author Contact Email aconsiglio[at]pcb[dot]ub[dot]es
PI Organization(En) -
PI Organization(Ja) -
PI Name(En) -
PI Name(Ja) -
PI Contact Email -
Availability Available
Provider Organization(En) Spanish Stem Cell Bank
Provider Organization(Ja) Spanish Stem Cell Bank
Provider Email aconsiglio[at]pcb[dot]ub[dot]es
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 22407749
DOI 10.1002/emmm.201200215
Title Disease-specific phenotypes in dopamine neurons from human iPS-based models of genetic and sporadic Parkinson's disease.
Authors Sanchez-Danes A, Richaud-Patin Y, Carballo-Carbajal I, Jimenez-Delgado S, Caig C, Mora S, Di Guglielmo C, Ezquerra M, Patel B, Giralt A, Canals JM, Memo M, Alberch J, Lopez-Barneo J, Vila M, Cuervo AM, Tolosa E, Consiglio A, Raya A
Journal EMBO Mol Med
Year 2012
Volume 4
Issue 5
Pages 380-95
URL http://www.ncbi.nlm.nih.gov/pubmed/22407749
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