SKIP000950
SKIP ID SKIP000950
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) dermal fibroblast
Cell Tissue(Ja) 表皮繊維芽細胞
Cell Origin Diseased
Cell Name 1(En) SP16.3
Cell Name 1(Ja) SP16.3
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) パーキンソン病
ICD Code 1 G20
Disease Name 1(En) Parkinson disease
OMIM1 168006
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age 51
Age Range 50-59
Sex Female
Race(En) -
Race(Ja) -
Genetic Diagnosis No
Not Detected No
Description(En) iPS cell line
Description(Ja) iPS細胞
Cell Morphology human ES-like
Grade Research Grade
Vector Retrovirus
Transgene SOX2, KLF4 and OCT3/4
Adhesiveness Yes
Feeder Yes
Feeder Cell MEFs
Medium Lines of patient-specific iPS cells were maintained by mechanical dissociation of colonies and splitting 1:3 onto feeder cells in hESC medium or by limited trypsin digestion and passaging onto Matrigel-coated plates with hESC medium pre-conditioned by mouse embryonic fibroblasts.
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay immunocytochemistry
in vitro Differentiation Yes
in vitro Differentiation Assay differentiation to three germ layers
in vivo Differentiation No
in vivo Differentiation Assay -
Other 1 Assay -
Other 1 Assay Method -
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay -
Remaining Vector Detection Yes
Remaining Vector Detection Assay qRT-PCR
STR -
HLA -
Stem Cell Transcriptome analysis Yes
Stem Cell Transcriptome analysis Assay qRT-PCR
Author Name(En) Adriana Sanchez-Danes
Author Name(Ja) Adriana Sanchez-Danes
Author Organization(En) Institute for Biomedicine (IBUB), University of Barcelona,
Author Organization(Ja) Institute for Biomedicine (IBUB), University of Barcelona,
Author Contact Email aconsiglio[at]pcb[dot]ub[dot]es
PI Organization(En) -
PI Organization(Ja) -
PI Name(En) -
PI Name(Ja) -
PI Contact Email -
Availability Available
Provider Organization(En) Spanish Stem Cell Bank
Provider Organization(Ja) Spanish Stem Cell Bank
Provider Email aconsiglio[at]pcb[dot]ub[dot]es
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 22407749
DOI 10.1002/emmm.201200215
Title Disease-specific phenotypes in dopamine neurons from human iPS-based models of genetic and sporadic Parkinson's disease.
Authors Sanchez-Danes A, Richaud-Patin Y, Carballo-Carbajal I, Jimenez-Delgado S, Caig C, Mora S, Di Guglielmo C, Ezquerra M, Patel B, Giralt A, Canals JM, Memo M, Alberch J, Lopez-Barneo J, Vila M, Cuervo AM, Tolosa E, Consiglio A, Raya A
Journal EMBO Mol Med
Year 2012
Volume 4
Issue 5
Pages 380-95
URL http://www.ncbi.nlm.nih.gov/pubmed/22407749
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