SKIP000809 |
SKIP ID |
SKIP000809 |
Organism(En) |
- |
Organism(Ja) |
- |
Cell Type(En) |
- |
Cell Type(Ja) |
- |
Cell Tissue(En) |
- |
Cell Tissue(Ja) |
- |
Cell Origin |
Diseased |
Cell Name 1(En) |
HPS0148 |
Cell Name 1(Ja) |
HPS0148 |
Cell Name 2(En) |
- |
Cell Name 2(Ja) |
- |
Disease Name 1(Ja) |
孤発性筋萎縮性側索硬化症 (bulbar, with UMN) |
ICD Code 1 |
- |
Disease Name 1(En) |
Amyotrophic lateral sclerosis, Sporadic type (bulbar, with UMN) |
OMIM1 |
- |
Disease Name 2(Ja) |
- |
ICD Code 2 |
- |
Disease Name 2(En) |
- |
OMIM 2 |
- |
Disease Name 3(Ja) |
- |
ICD Code 3 |
- |
Disease Name 3(En) |
- |
OMIM 3 |
- |
Age |
- |
Age Range |
-- |
Sex |
-- |
Race(En) |
Japanese |
Race(Ja) |
日本人 |
Genetic Diagnosis |
No |
Not Detected |
No |
Description(En) |
Disease specific iPS cell line derived from a patient : Amyotrophic lateral sclerosis, Sporadic type (bulbar, with UMN). HPS0146 and HPS0147 were derived from the same patient. iPS cells were created using the Sendai virus vector. Order Form to RIKEN BRC: C-0042, C-0057, C-0007 or C-0007p. |
Description(Ja) |
孤発性筋萎縮性側索硬化症 (bulbar, with UMN)患者由来。HPS0146、HPS0147と同一人由来。ガラス化法(Vitrification法)保存。提供依頼書式 (C-0041、C-0056、C-0003 または C-0003p、C-0058:必要な場合のみ)。 |
Cell Morphology |
human ES-like |
Grade |
Research Grade |
Vector |
Sendai virus |
Transgene |
SeV18+HS-OCT3/4/TSΔF, SeV18+HS-SOX2/TSΔF, SeV18+HS-KLF4/TSΔF, SeV18(HNL)c-MYCQC/TSΔF |
Adhesiveness |
- |
Feeder |
No |
Feeder Cell |
- |
Medium |
DMEM/HamF12(2mM L-Glutamine)+20%KSR+0.1mM NEAA+0.1mM 2-Mercaptoethanol+5ng/ml human bFGF. For subculture : once/4-6days. Medium change : every day |
Genome Editing |
No |
CO2 |
5% |
Mycoplasma |
Negative |
Detection of Contaminants Mycoplasma |
- |
Pluripotent Markers |
- |
Pluripotent Markers Assay |
- |
in vitro Differentiation |
- |
in vitro Differentiation Assay |
- |
in vivo Differentiation |
- |
in vivo Differentiation Assay |
- |
Other 1 Assay |
- |
Other 1 Assay Method |
- |
Other 2 Assay |
- |
Other 2 Assay Method |
- |
Other 3 Assay |
- |
Other 3 Assay Method |
- |
Karyotype |
- |
Karyotype Assay |
- |
Remaining Vector Detection |
- |
Remaining Vector Detection Assay |
- |
STR |
- |
HLA |
- |
Stem Cell Transcriptome analysis |
- |
Stem Cell Transcriptome analysis Assay |
- |
Author Name(En) |
- |
Author Name(Ja) |
- |
Author Organization(En) |
- |
Author Organization(Ja) |
- |
Author Contact Email |
- |
PI Organization(En) |
RIKEN BioResouce Center (RIKEN BRC) |
PI Organization(Ja) |
理化学研究所バイオリソースセンター |
PI Name(En) |
RIKEN BioResouce Center (RIKEN BRC) |
PI Name(Ja) |
理化学研究所バイオリソースセンター |
PI Contact Email |
- |
Availability |
Available |
Provider Organization(En) |
RIKEN BioResouce Center (RIKEN BRC) |
Provider Organization(Ja) |
理化学研究所バイオリソースセンター |
Provider Email |
cellips[at]brc[dot]riken[dot]jp |
Provider URL |
http://www2.brc.riken.jp/lab/cell/detail.cgi?cell_no=HPS0148&type=1 |
Ethical Statement(En) |
- |
Ethical Statement(Ja) |
- |
Terms of Use(En) |
- |
Terms of Use(Ja) |
- |
PubMed ID |
- |
DOI |
- |
Title |
- |
Authors |
- |
Journal |
- |
Year |
- |
Volume |
- |
Issue |
- |
Pages |
- |
URL |
- |
Free input |
- |
Note |
- |