| SKIP000823 | |
| SKIP ID | SKIP000823 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | - |
| Cell Tissue(Ja) | - |
| Cell Origin | Normal |
| Cell Name 1(En) | 418C-1 |
| Cell Name 1(Ja) | 418C-1 |
| Cell Name 2(En) | - |
| Cell Name 2(Ja) | - |
| Disease Name 1(Ja) | - |
| ICD Code 1 | - |
| Disease Name 1(En) | - |
| OMIM1 | - |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | 36 |
| Age Range | 30-39 |
| Sex | Male |
| Race(En) | Japanese |
| Race(Ja) | Japanese |
| Genetic Diagnosis | -- |
| Not Detected | No |
| Description(En) | Human iPS cell line, derived from fibloblast(JCRB TIG114). |
| Description(Ja) | TIG114線維芽細胞由来iPS細胞。エピゾーマルベクターによる樹立、導入細胞には導入遺伝子は残っていない。 |
| Cell Morphology | human ES-like |
| Grade | -- |
| Vector | Plasmid |
| Transgene | Episomal vector pCXLE, Oct3/4, Sox2, Klf4, L-Myc, Lin28, p53 shRNA |
| Adhesiveness | - |
| Feeder | Yes |
| Feeder Cell | SNL 76/7 (X-rays:5000R or MMC) 1.5-2.5x10^(6) cells/100mm dish |
| Medium | Primate ES Cell Medium+4ng/mL human basic FGF |
| Genome Editing | - |
| CO2 | - |
| Mycoplasma | - |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | Yes |
| Pluripotent Markers Assay | RT-PCR analysis |
| in vitro Differentiation | - |
| in vitro Differentiation Assay | - |
| in vivo Differentiation | Yes |
| in vivo Differentiation Assay | Teratoma Formation |
| Other 1 Assay | - |
| Other 1 Assay Method | - |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | Yes |
| Karyotype Assay | G-band |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | No |
| HLA | - |
| Stem Cell Transcriptome analysis | - |
| Stem Cell Transcriptome analysis Assay | - |
| Author Name(En) | Keisuke Okita |
| Author Name(Ja) | 沖田 圭介 |
| Author Organization(En) | Center for iPS Cell Research and Application, Kyoto University |
| Author Organization(Ja) | 京都大学iPS細胞研究所 |
| Author Contact Email | - |
| PI Organization(En) | Center for iPS Cell Research and Application, Kyoto University |
| PI Organization(Ja) | 京都大学iPS細胞研究所 |
| PI Name(En) | Shinya Yamanaka |
| PI Name(Ja) | 山中伸弥 |
| PI Contact Email | - |
| Availability | Information Only |
| Provider Organization(En) | Center for iPS Cell Research and Application, Kyoto University |
| Provider Organization(Ja) | 京都大学iPS細胞研究所 |
| Provider Email | - |
| Provider URL | http://www.cira.kyoto-u.ac.jp/ |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 21460823 |
| DOI | 10.1038/nmeth.1591 |
| Title | A more efficient method to generate integration-free human iPS cells. |
| Authors | Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S |
| Journal | Nat Methods |
| Year | 2011 |
| Volume | 8 |
| Issue | 5 |
| Pages | 409-12 |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/21460823 |
| Free input | - |
| Note | - |