SKIP000519
SKIP ID SKIP000519
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Diseased
Cell Name 1(En) PMD2-10
Cell Name 1(Ja) PMD2-10
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) ペリツェウス・メルツバッヘル病
ICD Code 1 E752
Disease Name 1(En) Pelizaeus-Merzbacher Disease
OMIM1 312080
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age 20
Age Range 20-29
Sex Male
Race(En) -
Race(Ja) -
Genetic Diagnosis Yes
Not Detected No
Description(En) Human iPSCs were established via the retroviral transduction of four transcription factors (SOX2, OCT4, KLF4, and c-MYC) into dermal fibroblasts.
iPSC lines, "PMD2-6" and "PMD2-22", were established from the same person.
Description(Ja) 皮膚線維芽細胞にレトロウイルスベクターを用いてSOX2, OCT4, KLF4, c-Mycの4因子を導入して樹立したヒト人工多能性幹細胞 (iPSC) 株。
PMD2-6、PMD2-22と同一人由来。
Cell Morphology human ES-like
Grade Research Grade
Vector Retrovirus
Transgene SOX2, OCT4, KLF4, c-Myc
Adhesiveness -
Feeder Yes
Feeder Cell Mitomycin-C-treated murine fibroblast feeder cells
Medium standard hESC medium (Dulbecco's modified Eagle's medium [DMEM]/F12 [Sigma] containing 20% KnockOut serum replacement [KSR; Life Technologies], nonessential amino acids [NEAA], 0.1 mM 2-mercaptoethanol [Sigma], and 4 ng/ml fibroblast growth factor 2 [FGF-2] [PeproTech])
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay -
in vitro Differentiation Yes
in vitro Differentiation Assay -
in vivo Differentiation Yes
in vivo Differentiation Assay teratoma formation
Other 1 Assay -
Other 1 Assay Method -
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype -
Karyotype Assay -
Remaining Vector Detection Yes
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Yohei Okada
Author Name(Ja) 岡田 洋平
Author Organization(En) Department of Physiology, Keio University School of Medicine
Author Organization(Ja) 慶應義塾大学 医学部 生理学教室
Author Contact Email yohei[at]a6[dot]keio[dot]jp
PI Organization(En) Department of Physiology, Keio University School of Medicine
PI Organization(Ja) 慶應義塾大学 医学部 生理学教室
PI Name(En) Hideyuki Okano
PI Name(Ja) 岡野 栄之
PI Contact Email hidokano[at]a2[dot]keio[dot]jp
Availability Information Only
Provider Organization(En) Department of Physiology, Keio University School of Medicine
Provider Organization(Ja) 慶應義塾大学 医学部 生理学教室
Provider Email yohei[at]a6[dot]keio[dot]jp
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 24936452
DOI 10.1016/j.stemcr.2014.03.007
Title Involvement of ER Stress in Dysmyelination of Pelizaeus-Merzbacher Disease with PLP1 Missense Mutations Shown by iPSC-Derived Oligodendrocytes.
Authors Numasawa-Kuroiwa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Osaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H
Journal Stem Cell Reports
Year 2014
Volume 2
Issue 5
Pages 648-61
URL http://www.ncbi.nlm.nih.gov/pubmed/24936452
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