SKIP000520 | |
SKIP ID | SKIP000520 |
Organism(En) | - |
Organism(Ja) | - |
Cell Type(En) | - |
Cell Type(Ja) | - |
Cell Tissue(En) | - |
Cell Tissue(Ja) | - |
Cell Origin | Diseased |
Cell Name 1(En) | PMD2-22 |
Cell Name 1(Ja) | PMD2-22 |
Cell Name 2(En) | - |
Cell Name 2(Ja) | - |
Disease Name 1(Ja) | ペリツェウス・メルツバッヘル病 |
ICD Code 1 | E752 |
Disease Name 1(En) | Pelizaeus-Merzbacher Disease |
OMIM1 | 312080 |
Disease Name 2(Ja) | - |
ICD Code 2 | - |
Disease Name 2(En) | - |
OMIM 2 | - |
Disease Name 3(Ja) | - |
ICD Code 3 | - |
Disease Name 3(En) | - |
OMIM 3 | - |
Age | 20 |
Age Range | 20-29 |
Sex | Male |
Race(En) | - |
Race(Ja) | - |
Genetic Diagnosis | Yes |
Not Detected | No |
Description(En) | Human iPSCs were established via the retroviral transduction of four transcription factors (SOX2, OCT4, KLF4, and c-MYC) into dermal fibroblasts. iPSC lines, "PMD2-6" and "PMD2-10", were established from the same person. |
Description(Ja) | 皮膚線維芽細胞にレトロウイルスベクターを用いてSOX2, OCT4, KLF4, c-Mycの4因子を導入して樹立したヒト人工多能性幹細胞 (iPSC) 株。 PMD2-6、PMD2-10と同一人由来。 |
Cell Morphology | human ES-like |
Grade | Research Grade |
Vector | Retrovirus |
Transgene | SOX2, OCT4, KLF4, c-Myc |
Adhesiveness | - |
Feeder | Yes |
Feeder Cell | Mitomycin-C-treated murine fibroblast feeder cells |
Medium | standard hESC medium (Dulbecco's modified Eagle's medium [DMEM]/F12 [Sigma] containing 20% KnockOut serum replacement [KSR; Life Technologies], nonessential amino acids [NEAA], 0.1 mM 2-mercaptoethanol [Sigma], and 4 ng/ml fibroblast growth factor 2 [FGF-2] [PeproTech]) |
Genome Editing | - |
CO2 | - |
Mycoplasma | - |
Detection of Contaminants Mycoplasma | - |
Pluripotent Markers | Yes |
Pluripotent Markers Assay | - |
in vitro Differentiation | Yes |
in vitro Differentiation Assay | - |
in vivo Differentiation | Yes |
in vivo Differentiation Assay | teratoma formation |
Other 1 Assay | - |
Other 1 Assay Method | - |
Other 2 Assay | - |
Other 2 Assay Method | - |
Other 3 Assay | - |
Other 3 Assay Method | - |
Karyotype | - |
Karyotype Assay | - |
Remaining Vector Detection | Yes |
Remaining Vector Detection Assay | - |
STR | - |
HLA | - |
Stem Cell Transcriptome analysis | - |
Stem Cell Transcriptome analysis Assay | - |
Author Name(En) | Yohei Okada |
Author Name(Ja) | 岡田 洋平 |
Author Organization(En) | Department of Physiology, Keio University School of Medicine |
Author Organization(Ja) | 慶應義塾大学 医学部 生理学教室 |
Author Contact Email | yohei[at]a6[dot]keio[dot]jp |
PI Organization(En) | Department of Physiology, Keio University School of Medicine |
PI Organization(Ja) | 慶應義塾大学 医学部 生理学教室 |
PI Name(En) | Hideyuki Okano |
PI Name(Ja) | 岡野 栄之 |
PI Contact Email | hidokano[at]a2[dot]keio[dot]jp |
Availability | Information Only |
Provider Organization(En) | Department of Physiology, Keio University School of Medicine |
Provider Organization(Ja) | 慶應義塾大学 医学部 生理学教室 |
Provider Email | yohei[at]a6[dot]keio[dot]jp |
Provider URL | - |
Ethical Statement(En) | - |
Ethical Statement(Ja) | - |
Terms of Use(En) | - |
Terms of Use(Ja) | - |
PubMed ID | 24936452 |
DOI | 10.1016/j.stemcr.2014.03.007 |
Title | Involvement of ER Stress in Dysmyelination of Pelizaeus-Merzbacher Disease with PLP1 Missense Mutations Shown by iPSC-Derived Oligodendrocytes. |
Authors | Numasawa-Kuroiwa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Osaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H |
Journal | Stem Cell Reports |
Year | 2014 |
Volume | 2 |
Issue | 5 |
Pages | 648-61 |
URL | http://www.ncbi.nlm.nih.gov/pubmed/24936452 |
Free input | - |
Note | - |