SKIP000528 | |
SKIP ID | SKIP000528 |
Organism(En) | - |
Organism(Ja) | - |
Cell Type(En) | - |
Cell Type(Ja) | - |
Cell Tissue(En) | - |
Cell Tissue(Ja) | - |
Cell Origin | Diseased |
Cell Name 1(En) | iPS MPD 183 clone 10 |
Cell Name 1(Ja) | iPS MPD 183 clone 10 |
Cell Name 2(En) | - |
Cell Name 2(Ja) | - |
Disease Name 1(Ja) | 骨髄増殖性疾患 |
ICD Code 1 | D47.1 |
Disease Name 1(En) | Myeloproliferative disorders |
OMIM1 | 263300 |
Disease Name 2(Ja) | 真性赤血球増加症 |
ICD Code 2 | D45 |
Disease Name 2(En) | Polycythemia vera |
OMIM 2 | 263300 |
Disease Name 3(Ja) | - |
ICD Code 3 | - |
Disease Name 3(En) | - |
OMIM 3 | - |
Age | - |
Age Range | -- |
Sex | Female |
Race(En) | - |
Race(Ja) | - |
Genetic Diagnosis | Yes |
Not Detected | No |
Description(En) | iPS cell lines from PB CD34+ cells containing the JAK2-V617F mutation that is commonly found in hematopoietic progenitor cells of adult patients with myeloproliferative disorders (MPDs). Four classic retroviral vectors pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, and pMXs-c-Myc encoding (mouse) reprogramming factors constructed by the laboratory of Dr Yamanaka were obtained from Addgene. |
Description(Ja) | JAK2-V617F変異(成人の骨髄増殖性疾患患者の造血前駆細胞によくみられる)を持つ末梢血CD34+細胞からiPS細胞を樹立した。 4つのレトロウイルスベクター(pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, and pMXs-c-Myc; 京大山中研のものをAddgeneにて購入)を用いてiPS細胞を樹立。 |
Cell Morphology | human ES-like |
Grade | Research Grade |
Vector | Retrovirus |
Transgene | pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, and pMXs-c-Myc |
Adhesiveness | - |
Feeder | Yes |
Feeder Cell | mouse embryonic fibroblast (MEF) |
Medium | the standard hES cell medium (knockout [KO] Dulbecco's modified Eagle's medium, 20% KO Serum Replacement (Invitrogen) with 4 ng/ml basic fibroblast growth factor) |
Genome Editing | - |
CO2 | - |
Mycoplasma | - |
Detection of Contaminants Mycoplasma | - |
Pluripotent Markers | Yes |
Pluripotent Markers Assay | - |
in vitro Differentiation | Yes |
in vitro Differentiation Assay | EB formation |
in vivo Differentiation | Yes |
in vivo Differentiation Assay | teratoma formation |
Other 1 Assay | - |
Other 1 Assay Method | - |
Other 2 Assay | - |
Other 2 Assay Method | - |
Other 3 Assay | - |
Other 3 Assay Method | - |
Karyotype | Yes |
Karyotype Assay | - |
Remaining Vector Detection | - |
Remaining Vector Detection Assay | - |
STR | - |
HLA | - |
Stem Cell Transcriptome analysis | - |
Stem Cell Transcriptome analysis Assay | - |
Author Name(En) | Linzhao Cheng |
Author Name(Ja) | Linzhao Cheng |
Author Organization(En) | Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine |
Author Organization(Ja) | Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine |
Author Contact Email | - |
PI Organization(En) | Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine |
PI Organization(Ja) | Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine |
PI Name(En) | Linzhao Cheng |
PI Name(Ja) | Linzhao Cheng |
PI Contact Email | - |
Availability | Available |
Provider Organization(En) | Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine |
Provider Organization(Ja) | Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine |
Provider Email | lcheng[at]welch[dot]jhu[dot]edu |
Provider URL | - |
Ethical Statement(En) | - |
Ethical Statement(Ja) | - |
Terms of Use(En) | - |
Terms of Use(Ja) | - |
PubMed ID | 19797525 -- 18723264 |
DOI | 10.1182/blood-2009-04-217406 -- 10.1016/j.exphem.2008.05.006 |
Title | Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders. -- Phenotypic variability within the JAK2 V617F-positive MPD: roles of progenitor cell and neutrophil allele burdens. |
Authors | Ye Z, Zhan H, Mali P, Dowey S, Williams DM, Jang YY, Dang CV, Spivak JL, Moliterno AR, Cheng L -- Moliterno AR, Williams DM, Rogers O, Isaacs MA, Spivak JL |
Journal | Blood -- Exp Hematol |
Year | 2009 -- 2008 |
Volume | 114 -- 36 |
Issue | 27 -- 11 |
Pages | 5473-80 -- 1480-6 |
URL | http://www.ncbi.nlm.nih.gov/pubmed/19797525 -- http://www.ncbi.nlm.nih.gov/pubmed/18723264 |
Free input | -- |
Note | - |