SKIP000530
SKIP ID SKIP000530
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Diseased
Cell Name 1(En) iPS MPD 562 clone 2
Cell Name 1(Ja) iPS MPD 562 clone 2
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) 骨髄増殖性疾患
ICD Code 1 D47.1
Disease Name 1(En) Myeloproliferative disorders
OMIM1 254450
Disease Name 2(Ja) 原発性骨髄線維症
ICD Code 2 D75.8
Disease Name 2(En) Primary myelofibrosis
OMIM 2 254450
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range --
Sex Female
Race(En) -
Race(Ja) -
Genetic Diagnosis Yes
Not Detected No
Description(En) iPS cell lines from PB CD34+ cells containing the JAK2-V617F mutation that is commonly found in hematopoietic progenitor cells of adult patients with myeloproliferative disorders (MPDs).
Four classic retroviral vectors pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, and pMXs-c-Myc encoding (mouse) reprogramming factors constructed by the laboratory of Dr Yamanaka were obtained from Addgene.
Description(Ja) JAK2-V617F変異(成人の骨髄増殖性疾患患者の造血前駆細胞によくみられる)を持つ末梢血CD34+細胞からiPS細胞を樹立した。
4つのレトロウイルスベクター(pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, and pMXs-c-Myc; 京大山中研のものをAddgeneにて購入)を用いてiPS細胞を樹立。
Cell Morphology human ES-like
Grade Research Grade
Vector Retrovirus
Transgene pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, and pMXs-c-Myc
Adhesiveness -
Feeder Yes
Feeder Cell mouse embryonic fibroblast (MEF)
Medium the standard hES cell medium (knockout [KO] Dulbecco's modified Eagle's medium, 20% KO Serum Replacement (Invitrogen) with 4 ng/ml basic fibroblast growth factor)
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay -
in vitro Differentiation Yes
in vitro Differentiation Assay EB formation
in vivo Differentiation Yes
in vivo Differentiation Assay teratoma formation
Other 1 Assay -
Other 1 Assay Method -
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay -
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) Linzhao Cheng
Author Name(Ja) Linzhao Cheng
Author Organization(En) Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine
Author Organization(Ja) Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine
Author Contact Email -
PI Organization(En) Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine
PI Organization(Ja) Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine
PI Name(En) Linzhao Cheng
PI Name(Ja) Linzhao Cheng
PI Contact Email -
Availability Information Only
Provider Organization(En) Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine
Provider Organization(Ja) Stem Cell Program, Institute for Cell Engineering, and Department of Gynecology & Obstetrics, Johns Hopkins University School of Medicine
Provider Email lcheng[at]welch[dot]jhu[dot]edu
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 19797525
--
18723264
DOI 10.1182/blood-2009-04-217406
--
10.1016/j.exphem.2008.05.006
Title Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders.
--
Phenotypic variability within the JAK2 V617F-positive MPD: roles of progenitor cell and neutrophil allele burdens.
Authors Ye Z, Zhan H, Mali P, Dowey S, Williams DM, Jang YY, Dang CV, Spivak JL, Moliterno AR, Cheng L
--
Moliterno AR, Williams DM, Rogers O, Isaacs MA, Spivak JL
Journal Blood
--
Exp Hematol
Year 2009
--
2008
Volume 114
--
36
Issue 27
--
11
Pages 5473-80
--
1480-6
URL http://www.ncbi.nlm.nih.gov/pubmed/19797525
--
http://www.ncbi.nlm.nih.gov/pubmed/18723264
Free input
--
Note -