| SKIP000593 | |
| SKIP ID | SKIP000593 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | - |
| Cell Tissue(Ja) | - |
| Cell Origin | Diseased |
| Cell Name 1(En) | PDB3F-9 |
| Cell Name 1(Ja) | PDB3F-9 |
| Cell Name 2(En) | - |
| Cell Name 2(Ja) | - |
| Disease Name 1(Ja) | パーキンソン病 |
| ICD Code 1 | G20 |
| Disease Name 1(En) | Parkinson disease |
| OMIM1 | 168600 |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | 53 |
| Age Range | 50-59 |
| Sex | Male |
| Race(En) | - |
| Race(Ja) | - |
| Genetic Diagnosis | Yes |
| Not Detected | No |
| Description(En) | Human iPSCs were established via a constitutively active lentivirus expressing the reverse tetracycline transactivator (FUW-M2rtTA) together with DOX-inducible lentiviruses transducing three (OCT4, SOX2, KLF4) reprogramming factors into AG20442 fibroblasts. PDB3F-iPSC lines were established from the same person. |
| Description(Ja) | 皮膚線維芽細胞（AG20442）にリバーステトラサイクリントランスアクチベーター（FUW-M2rtTA）とDOX誘導型3因子（SOX2, OCT4, KLF4）をのせたレンチウイルスベクターを用いて樹立したヒト人工多能性幹細胞 (iPSC) 株。 PDB3F-は同一人由来のクローン株。 |
| Cell Morphology | human ES-like |
| Grade | Research Grade |
| Vector | Lentivirus |
| Transgene | FUW-tetO 3 factors (OCT4, SOX2, KLF4) |
| Adhesiveness | - |
| Feeder | Yes |
| Feeder Cell | mitomycin C (MMC)-inactivated mouse embryonic fibroblast (MEF) feeder layers |
| Medium | hESC medium (DMEM/F12 [Invitrogen] supplemented with 15% FBS [Hyclone], 5% KnockOut Serum Replacement [Invitrogen], 1 mM glutamine [Invitrogen], 1% nonessential amino acids [Invitrogen], 0.1 mM β-mercaptoethanol [Sigma], and 4 ng/ml FGF2 [R&D Systems]) |
| Genome Editing | - |
| CO2 | - |
| Mycoplasma | - |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | Yes |
| Pluripotent Markers Assay | - |
| in vitro Differentiation | Yes |
| in vitro Differentiation Assay | - |
| in vivo Differentiation | Yes |
| in vivo Differentiation Assay | teratoma formation |
| Other 1 Assay | - |
| Other 1 Assay Method | - |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | Yes |
| Karyotype Assay | - |
| Remaining Vector Detection | Yes |
| Remaining Vector Detection Assay | - |
| STR | - |
| HLA | - |
| Stem Cell Transcriptome analysis | Yes |
| Stem Cell Transcriptome analysis Assay | Microarray Gene Expression Analysis |
| Author Name(En) | Rudolf Jaenisch |
| Author Name(Ja) | Rudolf Jaenisch |
| Author Organization(En) | The Whitehead Institute |
| Author Organization(Ja) | The Whitehead Institute |
| Author Contact Email | - |
| PI Organization(En) | The Whitehead Institute |
| PI Organization(Ja) | The Whitehead Institute |
| PI Name(En) | Rudolf Jaenisch |
| PI Name(Ja) | Rudolf Jaenisch |
| PI Contact Email | - |
| Availability | Available |
| Provider Organization(En) | The Whitehead Institute |
| Provider Organization(Ja) | The Whitehead Institute |
| Provider Email | jaenisch[at]wi[dot]mit[dot]edu |
| Provider URL | - |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 19269371 |
| DOI | 10.1016/j.cell.2009.02.013 |
| Title | Parkinson's disease patient-derived induced pluripotent stem cells free of viral reprogramming factors. |
| Authors | Soldner F, Hockemeyer D, Beard C, Gao Q, Bell GW, Cook EG, Hargus G, Blak A, Cooper O, Mitalipova M, Isacson O, Jaenisch R |
| Journal | Cell |
| Year | 2009 |
| Volume | 136 |
| Issue | 5 |
| Pages | 964-77 |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/19269371 |
| Free input | - |
| Note | - |