SKIP000608 | |
SKIP ID | SKIP000608 |
Organism(En) | - |
Organism(Ja) | - |
Cell Type(En) | - |
Cell Type(Ja) | - |
Cell Tissue(En) | - |
Cell Tissue(Ja) | - |
Cell Origin | Diseased |
Cell Name 1(En) | PDD4F-9 |
Cell Name 1(Ja) | PDD4F-9 |
Cell Name 2(En) | - |
Cell Name 2(Ja) | - |
Disease Name 1(Ja) | パーキンソン病 |
ICD Code 1 | G20 |
Disease Name 1(En) | Parkinson disease |
OMIM1 | 168600 |
Disease Name 2(Ja) | - |
ICD Code 2 | - |
Disease Name 2(En) | - |
OMIM 2 | - |
Disease Name 3(Ja) | - |
ICD Code 3 | - |
Disease Name 3(En) | - |
OMIM 3 | - |
Age | 60 |
Age Range | 60-69 |
Sex | Male |
Race(En) | - |
Race(Ja) | - |
Genetic Diagnosis | Yes |
Not Detected | No |
Description(En) | Human iPSCs were established via a constitutively active lentivirus expressing the reverse tetracycline transactivator (FUW-M2rtTA) together with DOX-inducible lentiviruses transducing four (OCT4, SOX2, KLF4, c-MYC) reprogramming factors into AG20445 fibroblasts. PDD-iPSC lines were established from the same person. |
Description(Ja) | 皮膚線維芽細胞(AG20445)にリバーステトラサイクリントランスアクチベーター(FUW-M2rtTA)とDOX誘導型4因子(SOX2, OCT4, KLF4, c-MYC)をのせたレンチウイルスベクターを用いて樹立したヒト人工多能性幹細胞 (iPSC) 株。 PDD-は同一人由来のクローン株。 |
Cell Morphology | human ES-like |
Grade | Research Grade |
Vector | Lentivirus |
Transgene | FUW-tetO 4 factors (OCT4, SOX2, KLF4, c-MYC) |
Adhesiveness | - |
Feeder | Yes |
Feeder Cell | mitomycin C (MMC)-inactivated mouse embryonic fibroblast (MEF) feeder layers |
Medium | hESC medium (DMEM/F12 [Invitrogen] supplemented with 15% FBS [Hyclone], 5% KnockOut Serum Replacement [Invitrogen], 1 mM glutamine [Invitrogen], 1% nonessential amino acids [Invitrogen], 0.1 mM β-mercaptoethanol [Sigma], and 4 ng/ml FGF2 [R&D Systems]) |
Genome Editing | - |
CO2 | - |
Mycoplasma | - |
Detection of Contaminants Mycoplasma | - |
Pluripotent Markers | Yes |
Pluripotent Markers Assay | - |
in vitro Differentiation | Yes |
in vitro Differentiation Assay | - |
in vivo Differentiation | Yes |
in vivo Differentiation Assay | teratoma formation |
Other 1 Assay | - |
Other 1 Assay Method | - |
Other 2 Assay | - |
Other 2 Assay Method | - |
Other 3 Assay | - |
Other 3 Assay Method | - |
Karyotype | Yes |
Karyotype Assay | - |
Remaining Vector Detection | Yes |
Remaining Vector Detection Assay | - |
STR | - |
HLA | - |
Stem Cell Transcriptome analysis | Yes |
Stem Cell Transcriptome analysis Assay | Microarray Gene Expression Analysis |
Author Name(En) | Rudolf Jaenisch |
Author Name(Ja) | Rudolf Jaenisch |
Author Organization(En) | The Whitehead Institute |
Author Organization(Ja) | The Whitehead Institute |
Author Contact Email | - |
PI Organization(En) | The Whitehead Institute |
PI Organization(Ja) | The Whitehead Institute |
PI Name(En) | Rudolf Jaenisch |
PI Name(Ja) | Rudolf Jaenisch |
PI Contact Email | - |
Availability | Available |
Provider Organization(En) | The Whitehead Institute |
Provider Organization(Ja) | The Whitehead Institute |
Provider Email | jaenisch[at]wi[dot]mit[dot]edu |
Provider URL | - |
Ethical Statement(En) | - |
Ethical Statement(Ja) | - |
Terms of Use(En) | - |
Terms of Use(Ja) | - |
PubMed ID | 19269371 |
DOI | 10.1016/j.cell.2009.02.013 |
Title | Parkinson's disease patient-derived induced pluripotent stem cells free of viral reprogramming factors. |
Authors | Soldner F, Hockemeyer D, Beard C, Gao Q, Bell GW, Cook EG, Hargus G, Blak A, Cooper O, Mitalipova M, Isacson O, Jaenisch R |
Journal | Cell |
Year | 2009 |
Volume | 136 |
Issue | 5 |
Pages | 964-77 |
URL | http://www.ncbi.nlm.nih.gov/pubmed/19269371 |
Free input | - |
Note | - |