SKIP000976 | |
SKIP ID | SKIP000976 |
Organism(En) | - |
Organism(Ja) | - |
Cell Type(En) | - |
Cell Type(Ja) | - |
Cell Tissue(En) | - |
Cell Tissue(Ja) | - |
Cell Origin | Normal |
Cell Name 1(En) | Control-2#13 |
Cell Name 1(Ja) | Control-2#13 |
Cell Name 2(En) | - |
Cell Name 2(Ja) | - |
Disease Name 1(Ja) | - |
ICD Code 1 | - |
Disease Name 1(En) | - |
OMIM1 | - |
Disease Name 2(Ja) | - |
ICD Code 2 | - |
Disease Name 2(En) | - |
OMIM 2 | - |
Disease Name 3(Ja) | - |
ICD Code 3 | - |
Disease Name 3(En) | - |
OMIM 3 | - |
Age | 39 |
Age Range | 30-39 |
Sex | Female |
Race(En) | - |
Race(Ja) | - |
Genetic Diagnosis | -- |
Not Detected | No |
Description(En) | Transgene-free iPSCs from fibroblasts using Cre-excisable lentiviruses (loxP-TetO-OKSM, loxP-FUW-M2rtTA). iPSC line, Control-2x, was established from the same person's fibroblasts, GM23151. |
Description(Ja) | 皮膚線維芽細胞にCreで排除可能な特定の配列(loxP-TetO-OKSM, loxP-FUW-M2rtTA)をのせたレンチウイルスを用いてトランスジーンが残存しないiPSCを樹立した。 Control-2xは同一ヒト皮膚線維芽細胞(GM23151)由来のクローン株。 |
Cell Morphology | human ES-like |
Grade | Research Grade |
Vector | Lentivirus |
Transgene | loxP-TetO-OKSM, loxP-FUW-M2rtTA |
Adhesiveness | - |
Feeder | Yes |
Feeder Cell | - |
Medium | - |
Genome Editing | - |
CO2 | - |
Mycoplasma | Unknown |
Detection of Contaminants Mycoplasma | - |
Pluripotent Markers | Yes |
Pluripotent Markers Assay | immunocytochemistry |
in vitro Differentiation | Yes |
in vitro Differentiation Assay | hepatic and neuronal differentiation |
in vivo Differentiation | Yes |
in vivo Differentiation Assay | teratoma formation assay |
Other 1 Assay | - |
Other 1 Assay Method | - |
Other 2 Assay | - |
Other 2 Assay Method | - |
Other 3 Assay | - |
Other 3 Assay Method | - |
Karyotype | Yes |
Karyotype Assay | karyotype analysis |
Remaining Vector Detection | Yes |
Remaining Vector Detection Assay | Southern blot analysis |
STR | Yes |
HLA | - |
Stem Cell Transcriptome analysis | Yes |
Stem Cell Transcriptome analysis Assay | qRT-PCR |
Author Name(En) | Rudolf Jaenisch |
Author Name(Ja) | Rudolf Jaenisch |
Author Organization(En) | Whitehead Institute for Biomedical Research |
Author Organization(Ja) | Whitehead Institute for Biomedical Research |
Author Contact Email | jaenisch[at]wi[dot]mit[dot]edu |
PI Organization(En) | Whitehead Institute for Biomedical Research |
PI Organization(Ja) | Whitehead Institute for Biomedical Research |
PI Name(En) | Rudolf Jaenisch |
PI Name(Ja) | Rudolf Jaenisch |
PI Contact Email | jaenisch[at]wi[dot]mit[dot]edu |
Availability | Information Only |
Provider Organization(En) | Whitehead Institute for Biomedical Research |
Provider Organization(Ja) | Whitehead Institute for Biomedical Research |
Provider Email | jaenisch[at]wi[dot]mit[dot]edu |
Provider URL | - |
Ethical Statement(En) | - |
Ethical Statement(Ja) | - |
Terms of Use(En) | - |
Terms of Use(Ja) | - |
PubMed ID | 24936472 |
DOI | 10.1016/j.stemcr.2014.03.014 |
Title | Genetic and chemical correction of cholesterol accumulation and impaired autophagy in hepatic and neural cells derived from Niemann-Pick Type C patient-specific iPS cells. |
Authors | Maetzel D, Sarkar S, Wang H, Abi-Mosleh L, Xu P, Cheng AW, Gao Q, Mitalipova M, Jaenisch R |
Journal | Stem Cell Reports |
Year | 2014 |
Volume | 2 |
Issue | 6 |
Pages | 866-80 |
URL | http://www.ncbi.nlm.nih.gov/pubmed/24936472 |
Free input | - |
Note | - |