SKIP000976
SKIP ID SKIP000976
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Normal
Cell Name 1(En) Control-2#13
Cell Name 1(Ja) Control-2#13
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age 39
Age Range 30-39
Sex Female
Race(En) -
Race(Ja) -
Genetic Diagnosis --
Not Detected No
Description(En) Transgene-free iPSCs from fibroblasts using Cre-excisable lentiviruses (loxP-TetO-OKSM, loxP-FUW-M2rtTA).
iPSC line, Control-2x, was established from the same person's fibroblasts, GM23151.
Description(Ja) 皮膚線維芽細胞にCreで排除可能な特定の配列(loxP-TetO-OKSM, loxP-FUW-M2rtTA)をのせたレンチウイルスを用いてトランスジーンが残存しないiPSCを樹立した。
Control-2xは同一ヒト皮膚線維芽細胞(GM23151)由来のクローン株。
Cell Morphology human ES-like
Grade Research Grade
Vector Lentivirus
Transgene loxP-TetO-OKSM, loxP-FUW-M2rtTA
Adhesiveness -
Feeder Yes
Feeder Cell -
Medium -
Genome Editing -
CO2 -
Mycoplasma Unknown
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay immunocytochemistry
in vitro Differentiation Yes
in vitro Differentiation Assay hepatic and neuronal differentiation
in vivo Differentiation Yes
in vivo Differentiation Assay teratoma formation assay
Other 1 Assay -
Other 1 Assay Method -
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay karyotype analysis
Remaining Vector Detection Yes
Remaining Vector Detection Assay Southern blot analysis
STR Yes
HLA -
Stem Cell Transcriptome analysis Yes
Stem Cell Transcriptome analysis Assay qRT-PCR
Author Name(En) Rudolf Jaenisch
Author Name(Ja) Rudolf Jaenisch
Author Organization(En) Whitehead Institute for Biomedical Research
Author Organization(Ja) Whitehead Institute for Biomedical Research
Author Contact Email jaenisch[at]wi[dot]mit[dot]edu
PI Organization(En) Whitehead Institute for Biomedical Research
PI Organization(Ja) Whitehead Institute for Biomedical Research
PI Name(En) Rudolf Jaenisch
PI Name(Ja) Rudolf Jaenisch
PI Contact Email jaenisch[at]wi[dot]mit[dot]edu
Availability Information Only
Provider Organization(En) Whitehead Institute for Biomedical Research
Provider Organization(Ja) Whitehead Institute for Biomedical Research
Provider Email jaenisch[at]wi[dot]mit[dot]edu
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 24936472
DOI 10.1016/j.stemcr.2014.03.014
Title Genetic and chemical correction of cholesterol accumulation and impaired autophagy in hepatic and neural cells derived from Niemann-Pick Type C patient-specific iPS cells.
Authors Maetzel D, Sarkar S, Wang H, Abi-Mosleh L, Xu P, Cheng AW, Gao Q, Mitalipova M, Jaenisch R
Journal Stem Cell Reports
Year 2014
Volume 2
Issue 6
Pages 866-80
URL http://www.ncbi.nlm.nih.gov/pubmed/24936472
Free input -
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