SKIP000969 | |
SKIP ID | SKIP000969 |
Organism(En) | - |
Organism(Ja) | - |
Cell Type(En) | - |
Cell Type(Ja) | - |
Cell Tissue(En) | - |
Cell Tissue(Ja) | - |
Cell Origin | Diseased |
Cell Name 1(En) | PDB1lox-21Puro-13 |
Cell Name 1(Ja) | PDB1lox-21Puro-13 |
Cell Name 2(En) | - |
Cell Name 2(Ja) | - |
Disease Name 1(Ja) | パーキンソン病 |
ICD Code 1 | G20 |
Disease Name 1(En) | Parkinson disease |
OMIM1 | 168600 |
Disease Name 2(Ja) | - |
ICD Code 2 | - |
Disease Name 2(En) | - |
OMIM 2 | - |
Disease Name 3(Ja) | - |
ICD Code 3 | - |
Disease Name 3(En) | - |
OMIM 3 | - |
Age | 53 |
Age Range | 50-59 |
Sex | Male |
Race(En) | - |
Race(Ja) | - |
Genetic Diagnosis | Yes |
Not Detected | No |
Description(En) | Human iPSCs were established from AG20442 fibroblasts. In order to derive hiPSCs that were free of proviruses, estabulishers generated lentiviral vectors that could be excised after integration using Cre-recombinase. The human ubiquitin promoter of the FUGW-loxP lentivirus, which contains a loxP site in the 30 long terminal repeat (LTR), was replaced with a DOX-inducible, minimal cytomegalovirus (CMV) promoter followed by the human cDNAs for OCT4, KLF4, or SOX2. Upon proviral replication, the loxP site in the 30 LTR is duplicated into the 50 LTR, resulting in an integrated transgene flanked by loxP sites in both LTRs. Such a Cre-loxP system worked on this iPSC line, resulting in reprogramming factors were removed. iPSC lines, PDB^x, were established from the same person. |
Description(Ja) | 皮膚線維芽細胞(AG20442)より樹立したヒト人工多能性幹細胞 (iPSC) 株。 ウイルス由来の導入遺伝子を排除したiPSC株を作製するために樹立者らはCre-recombinaseを用いてゲノムインテグレーションを排除するレンチウイルスベクターを作製した。30 LTRにloxPを含むFUGW-loxPレンチウイルスのヒトユビキチンプロモーターはヒトcDNA(OCT4, KLF4, or SOX2)をもつドキシサイクリン誘導性CMVプロモーターに置換される。ウイルス複製の際に、30 LTRのloxPサイトは50 LTRに複製される。結果的に導入遺伝子は両LTRの間に配置される。 本株は上記のシステムを稼働させ、初期化因子を排除したものである。 PDB^xは同一人由来のクローン株。 |
Cell Morphology | human ES-like |
Grade | Research Grade |
Vector | Lentivirus |
Transgene | FUWtetO-loxP lentiviral vectors transducing three reprogramming factors, OCT4, KLF4, and SOX2. (Reprogramming factors were removed by Cre-recombinase.) |
Adhesiveness | - |
Feeder | Yes |
Feeder Cell | mitomycin C (MMC)-inactivated mouse embryonic fibroblast (MEF) feeder layers |
Medium | hESC medium (DMEM/F12 [Invitrogen] supplemented with 15% FBS [Hyclone], 5% KnockOut Serum Replacement [Invitrogen], 1 mM glutamine [Invitrogen], 1% nonessential amino acids [Invitrogen], 0.1 mM β-mercaptoethanol [Sigma], and 4 ng/ml FGF2 [R&D Systems]) |
Genome Editing | pCre-PAC |
CO2 | - |
Mycoplasma | - |
Detection of Contaminants Mycoplasma | - |
Pluripotent Markers | Yes |
Pluripotent Markers Assay | immunocytochemistry |
in vitro Differentiation | Yes |
in vitro Differentiation Assay | differentiation to dopmainargic neuron |
in vivo Differentiation | Yes |
in vivo Differentiation Assay | teratoma formation |
Other 1 Assay | - |
Other 1 Assay Method | - |
Other 2 Assay | - |
Other 2 Assay Method | - |
Other 3 Assay | - |
Other 3 Assay Method | - |
Karyotype | Yes |
Karyotype Assay | - |
Remaining Vector Detection | Yes |
Remaining Vector Detection Assay | Southern blot analysis |
STR | - |
HLA | - |
Stem Cell Transcriptome analysis | Yes |
Stem Cell Transcriptome analysis Assay | qRT-PCR |
Author Name(En) | Rudolf Jaenisch |
Author Name(Ja) | Rudolf Jaenisch |
Author Organization(En) | The Whitehead Institute |
Author Organization(Ja) | The Whitehead Institute |
Author Contact Email | - |
PI Organization(En) | The Whitehead Institute |
PI Organization(Ja) | The Whitehead Institute |
PI Name(En) | Rudolf Jaenisch |
PI Name(Ja) | Rudolf Jaenisch |
PI Contact Email | - |
Availability | Available |
Provider Organization(En) | The Whitehead Institute |
Provider Organization(Ja) | The Whitehead Institute |
Provider Email | jaenisch[at]wi[dot]mit[dot]edu |
Provider URL | - |
Ethical Statement(En) | - |
Ethical Statement(Ja) | - |
Terms of Use(En) | - |
Terms of Use(Ja) | - |
PubMed ID | 19269371 |
DOI | 10.1016/j.cell.2009.02.013 |
Title | Parkinson's disease patient-derived induced pluripotent stem cells free of viral reprogramming factors. |
Authors | Soldner F, Hockemeyer D, Beard C, Gao Q, Bell GW, Cook EG, Hargus G, Blak A, Cooper O, Mitalipova M, Isacson O, Jaenisch R |
Journal | Cell |
Year | 2009 |
Volume | 136 |
Issue | 5 |
Pages | 964-77 |
URL | http://www.ncbi.nlm.nih.gov/pubmed/19269371 |
Free input | - |
Note | - |