| SKIP000277 | |
| SKIP ID | SKIP000277 |
| Organism(En) | - |
| Organism(Ja) | - |
| Cell Type(En) | - |
| Cell Type(Ja) | - |
| Cell Tissue(En) | - |
| Cell Tissue(Ja) | - |
| Cell Origin | Diseased |
| Cell Name 1(En) | KO-001-25 |
| Cell Name 1(Ja) | KO-001-25 |
| Cell Name 2(En) | - |
| Cell Name 2(Ja) | - |
| Disease Name 1(Ja) | 統合失調症 |
| ICD Code 1 | F209 |
| Disease Name 1(En) | Schizophrenia |
| OMIM1 | 181500 |
| Disease Name 2(Ja) | - |
| ICD Code 2 | - |
| Disease Name 2(En) | - |
| OMIM 2 | - |
| Disease Name 3(Ja) | - |
| ICD Code 3 | - |
| Disease Name 3(En) | - |
| OMIM 3 | - |
| Age | - |
| Age Range | 30-39 |
| Sex | Female |
| Race(En) | - |
| Race(Ja) | - |
| Genetic Diagnosis | Yes |
| Not Detected | No |
| Description(En) | 22q11.2 deletion |
| Description(Ja) | 22q11.2 欠損 |
| Cell Morphology | human ES-like |
| Grade | -- |
| Vector | Retrovirus |
| Transgene | pMXs-Oct3/4, pMXs-Sox2, pMXs-Klf4, pMXs-c-Myc |
| Adhesiveness | Yes |
| Feeder | Yes |
| Feeder Cell | SNL 1.5x10^(6)/10cm dish, Mitomycin C treatment:400microG/ml, 2hr 15min |
| Medium | DMEM/F12(SIGMA; D6421) |
| Genome Editing | - |
| CO2 | - |
| Mycoplasma | - |
| Detection of Contaminants Mycoplasma | - |
| Pluripotent Markers | Yes |
| Pluripotent Markers Assay | IHC |
| in vitro Differentiation | Yes |
| in vitro Differentiation Assay | IHC |
| in vivo Differentiation | - |
| in vivo Differentiation Assay | - |
| Other 1 Assay | - |
| Other 1 Assay Method | - |
| Other 2 Assay | - |
| Other 2 Assay Method | - |
| Other 3 Assay | - |
| Other 3 Assay Method | - |
| Karyotype | - |
| Karyotype Assay | - |
| Remaining Vector Detection | - |
| Remaining Vector Detection Assay | - |
| STR | - |
| HLA | - |
| Stem Cell Transcriptome analysis | - |
| Stem Cell Transcriptome analysis Assay | - |
| Author Name(En) | Hideyuki Okano -- Takeio Yoshikawa |
| Author Name(Ja) | 岡野 栄之 -- 吉川武男 |
| Author Organization(En) | Keio University -- Riken Brain Science Institute |
| Author Organization(Ja) | 慶應義塾大学 -- 理研BSI |
| Author Contact Email | -- takeo[at]brain[dot]riken[dot]jp |
| PI Organization(En) | Keio University |
| PI Organization(Ja) | 慶應義塾大学 |
| PI Name(En) | Hideyuki Okano |
| PI Name(Ja) | 岡野 栄之 |
| PI Contact Email | - |
| Availability | Not Available |
| Provider Organization(En) | Keio University |
| Provider Organization(Ja) | 慶應義塾大学医学部 |
| Provider Email | akamatsu[at]a7[dot]keio[dot]jp |
| Provider URL | - |
| Ethical Statement(En) | - |
| Ethical Statement(Ja) | - |
| Terms of Use(En) | - |
| Terms of Use(Ja) | - |
| PubMed ID | 24389010 |
| DOI | 10.1016/j.neuron.2013.10.053 |
| Title | Increased l1 retrotransposition in the neuronal genome in schizophrenia. |
| Authors | Bundo M, Toyoshima M, Okada Y, Akamatsu W, Ueda J, Nemoto-Miyauchi T, Sunaga F, Toritsuka M, Ikawa D, Kakita A, Kato M, Kasai K, Kishimoto T, Nawa H, Okano H, Yoshikawa T, Kato T, Iwamoto K |
| Journal | Neuron |
| Year | 2014 |
| Volume | 81 |
| Issue | 2 |
| Pages | 306-13 |
| URL | http://www.ncbi.nlm.nih.gov/pubmed/24389010 |
| Free input | - |
| Note | 確定診断方法：DSM-IV(22q11.2 欠失はFISHにて確認) HIV : HTLV-1 : HBV : HCV : その他の感染症 : 梅毒 陰性（梅毒定性RPR（ラテックス比濁法）、梅毒定性TP抗体（ラテックス比濁法） 病歴・治療歴等 : 28歳時、注意力の低下、記憶障害、独語、空笑、幻聴などの精神病症状が亜急性に出現。 精神科受診し統合失調症との診断を受ける。非定型抗精神病薬等で治療した結果、独語、 空笑等の症状は軽快した。 その他の既往歴 : VSD、口蓋裂（構音障害）、橋本病、学習障害 家族歴 : 特記事項なし。 飲酒・喫煙の嗜好歴 : なし 身体検査所見 : 長細い鼻、短い眼列という特徴的な顔貌あり。足指の短小化、肥満傾向あり。 月経は正常。 臨床検査データ : VIQ 66, PIQ 54, Full IQ 57 維持培養方法 1.基本培地 DMEM/F12(SIGMA; D6421) 2.血清: 使用しない 3.添加物: 使用する KockOutTM Serum Replecement(GIBCO; 10828-028) 20% Non Essential Amino Acid Solution(SIGMA; M7145) 10% b-mercaptoethanol(SIGMA; M7522) 0.1% 200mM L-Glutamine(Nacalai tesque; 16948-04) 1% human-FGF basic(PEPRO TECH; 100-18B)4ng/mL 4.抗生物質（含抗真菌剤） : 使用する Penicillin/Streptomycin(Nacalai tesque; 26253-84) 0.5% 5.培養温度 : 37℃ 6.CO2濃度 : 3.0% 7.培養容器 : 10 cm dish(日本ジェネティクス; FG-2090) コーティングの有無 : コーティングする Gelatin from porcine skin Type A(SIGMA; G2625) 8.Feeder Cellの使用: 使用する 細胞名 : SNL細胞 使用時の細胞密度 : 1.5 x 10^(6)/10cm dish 使用前処理方法 : Mitomycin C処理 : 400microG/mL, 2hr 15min 9.継代方法 [1]Aspirate medium. [2]Wash once with PBS. [3]Add Dissociation solution 1mL. [4]Aspirate Dissociation solution. [5]Incubate 7min at 37 degree. [6]Add 6ml Medium and collect colonies by mild pipetting. [7]Spin down at 160g 5min. [8]Aspirate supernatant and add appropriate volume of hiPS medium. [9]Dissociate colonies by Pipetting 5-6 times with 10ml pipette.(dissociate into small cell clamps) [10]Passage the cells 1:3-1:8. Culture at 37 degree, 3% CO2. *10mL plastic disposable pipette(greiner; 607160) *Dissociation solution 2.5% Trypsin 5ml(GIBCO; 15090-046) 1mg/mL Coll IV 5ml(GIBCO; 17104-019) 1M CaCl2 0.05ml(Nacalai tesque; 06729-55) KSR 10ml(GIBCO; 10828-028) Total 50ml Aliquot and store at -20 degree 10.パッセージの際の細胞播種時の細胞密度 Confluentの10cm dish 1枚から、10cm dish 3〜8枚にパッセージ 11.継代頻度 : コロニーが分化する直前 コロニーの中心および辺縁が茶色くなり始めた時点 4-6日に一回 12.その他 凍結保存方法 ・ガラス化法 [1]Wash cells once with PBS, add and aspirate dissociation solution, incubate 7min at 37 degree, and add 6mL of hiPS medium per 10cm dish. [2]Carefully detach colonies with cell scraper(keep original colony size). [3]Collect medium + colonies using 5mL pipette(grainer-important point). [4]Divide 6mL colony suspension into 2mL x 3 tupes(15mL falcons) and spin at 160 g for 5min at 4 degree. [5]Aspirate medium and put tubes with peletts on ice. Freezing procedure: [6]Take 200 microL of freezing solution using a P1000 pipette, and reset the dial to around 300 microL. [7]Resuspend pellet and pipette once or twice and immediately transfer to cryotube. [8]Immediately transfer cryotube directly to flask containing liquid N2 for 15s abd drio the tupe into liquid N2. [9]Steps 8-9 must be performed within 15 seconds. ・Freezing solution(DAP213) [1]0.59g 1M acetamide to 6mL of hiPS medium to 15ml tube [2]Add 1.42mL 2M DMSO & 2.2mL Propylene glycol [3]Measure up to the 10mL line by adding hiPS medium Sterile filter through 0.2microM filters, Store at -80℃ Acetamide(SIGMA;A0500) Dimethyl Sulfoxide(SIGMA;D5879) Propylene glycol(SIGMA;398039) 0.2microM filter(Millipore;SLGV033RB) 継代数 : 継代10回目 確認済の未分化マーカー Oct3/4 : ICC法 Nanog : ICC法 Tra-1-60 : ICC法 染色体解析 : 未実施 胚葉体形成実験 : 実施 テラトーマ形成実験 : 未実施 in vitro分化能解析 : 実施 マイコプラズマ汚染検査 : 未実施 細胞同定検査（STR多型解析） : クローニング : |