SKIP000890 | |
SKIP ID | SKIP000890 |
Organism(En) | - |
Organism(Ja) | - |
Cell Type(En) | - |
Cell Type(Ja) | - |
Cell Tissue(En) | - |
Cell Tissue(Ja) | - |
Cell Origin | Normal |
Cell Name 1(En) | 7H5 |
Cell Name 1(Ja) | 7H5 |
Cell Name 2(En) | - |
Cell Name 2(Ja) | - |
Disease Name 1(Ja) | - |
ICD Code 1 | - |
Disease Name 1(En) | - |
OMIM1 | - |
Disease Name 2(Ja) | - |
ICD Code 2 | - |
Disease Name 2(En) | - |
OMIM 2 | - |
Disease Name 3(Ja) | - |
ICD Code 3 | - |
Disease Name 3(En) | - |
OMIM 3 | - |
Age | 36 |
Age Range | 30-39 |
Sex | Female |
Race(En) | - |
Race(Ja) | - |
Genetic Diagnosis | -- |
Not Detected | No |
Description(En) | iPSC derived from primary human dermal fibroblasts, generated with SeV (c-Myc were inserted at 18+) |
Description(Ja) | ヒト線維芽細胞由来iPS細胞 SeVを用いて作製(18塩基目にc-Mycを挿入) |
Cell Morphology | human ES-like |
Grade | Research Grade |
Vector | Sendai virus |
Transgene | Oct3/4,Sox2,Klf4,c-Myc |
Adhesiveness | - |
Feeder | Yes |
Feeder Cell | MMC-treated MEF(ReproCELL) |
Medium | primate ES medium (ReproCELL) supplemented with 10 ng/ml bFGF (R&D systems) |
Genome Editing | - |
CO2 | - |
Mycoplasma | - |
Detection of Contaminants Mycoplasma | - |
Pluripotent Markers | Yes |
Pluripotent Markers Assay | RT-PCR, IH, WB, telomerase |
in vitro Differentiation | Yes |
in vitro Differentiation Assay | EB formation |
in vivo Differentiation | Yes |
in vivo Differentiation Assay | teratoma formation |
Other 1 Assay | Yes |
Other 1 Assay Method | cardiomyocytes |
Other 2 Assay | Yes |
Other 2 Assay Method | PA6 |
Other 3 Assay | - |
Other 3 Assay Method | - |
Karyotype | Yes |
Karyotype Assay | G-banding |
Remaining Vector Detection | Yes |
Remaining Vector Detection Assay | RT-PCR, RT-qPCR, Western Blotting, Anti-Sev-immunostaining |
STR | - |
HLA | - |
Stem Cell Transcriptome analysis | Yes |
Stem Cell Transcriptome analysis Assay | DNA fingerprinting |
Author Name(En) | Noemi Fusaki |
Author Name(Ja) | 房木 ノエミ |
Author Organization(En) | DNAVEC Corporation |
Author Organization(Ja) | (株)IDファーマ |
Author Contact Email | - |
PI Organization(En) | - |
PI Organization(Ja) | - |
PI Name(En) | - |
PI Name(Ja) | - |
PI Contact Email | - |
Availability | Information Only |
Provider Organization(En) | - |
Provider Organization(Ja) | - |
Provider Email | - |
Provider URL | - |
Ethical Statement(En) | - |
Ethical Statement(Ja) | - |
Terms of Use(En) | - |
Terms of Use(Ja) | - |
PubMed ID | 19838014 |
DOI | 10.2183/pjab.85.348 |
Title | Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome. |
Authors | Fusaki N, Ban H, Nishiyama A, Saeki K, Hasegawa M |
Journal | Proc Jpn Acad Ser B Phys Biol Sci |
Year | 2009 |
Volume | 85 |
Issue | 8 |
Pages | 348-62 |
URL | http://www.ncbi.nlm.nih.gov/pubmed/19838014 |
Free input | - |
Note | - |