SKIP000890
SKIP ID SKIP000890
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Normal
Cell Name 1(En) 7H5
Cell Name 1(Ja) 7H5
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age 36
Age Range 30-39
Sex Female
Race(En) -
Race(Ja) -
Genetic Diagnosis --
Not Detected No
Description(En) iPSC derived from primary human dermal fibroblasts,
generated with SeV
(c-Myc were inserted at 18+)
Description(Ja) ヒト線維芽細胞由来iPS細胞
SeVを用いて作製(18塩基目にc-Mycを挿入)
Cell Morphology human ES-like
Grade Research Grade
Vector Sendai virus
Transgene Oct3/4,Sox2,Klf4,c-Myc
Adhesiveness -
Feeder Yes
Feeder Cell MMC-treated MEF(ReproCELL)
Medium primate ES medium (ReproCELL) supplemented with 10 ng/ml bFGF (R&D systems)
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay RT-PCR, IH, WB, telomerase
in vitro Differentiation Yes
in vitro Differentiation Assay EB formation
in vivo Differentiation Yes
in vivo Differentiation Assay teratoma formation
Other 1 Assay Yes
Other 1 Assay Method cardiomyocytes
Other 2 Assay Yes
Other 2 Assay Method PA6
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay G-banding
Remaining Vector Detection Yes
Remaining Vector Detection Assay RT-PCR, RT-qPCR, Western Blotting, Anti-Sev-immunostaining
STR -
HLA -
Stem Cell Transcriptome analysis Yes
Stem Cell Transcriptome analysis Assay DNA fingerprinting
Author Name(En) Noemi Fusaki
Author Name(Ja) 房木 ノエミ
Author Organization(En) DNAVEC Corporation
Author Organization(Ja) (株)IDファーマ
Author Contact Email -
PI Organization(En) -
PI Organization(Ja) -
PI Name(En) -
PI Name(Ja) -
PI Contact Email -
Availability Information Only
Provider Organization(En) -
Provider Organization(Ja) -
Provider Email -
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 19838014
DOI 10.2183/pjab.85.348
Title Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome.
Authors Fusaki N, Ban H, Nishiyama A, Saeki K, Hasegawa M
Journal Proc Jpn Acad Ser B Phys Biol Sci
Year 2009
Volume 85
Issue 8
Pages 348-62
URL http://www.ncbi.nlm.nih.gov/pubmed/19838014
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