SKIP000673 | |
SKIP ID | SKIP000673 |
Organism(En) | - |
Organism(Ja) | - |
Cell Type(En) | - |
Cell Type(Ja) | - |
Cell Tissue(En) | - |
Cell Tissue(Ja) | - |
Cell Origin | Normal |
Cell Name 1(En) | dH1f-RiPS-1.14 |
Cell Name 1(Ja) | dH1f-RiPS-1.14 |
Cell Name 2(En) | - |
Cell Name 2(Ja) | - |
Disease Name 1(Ja) | - |
ICD Code 1 | - |
Disease Name 1(En) | - |
OMIM1 | - |
Disease Name 2(Ja) | - |
ICD Code 2 | - |
Disease Name 2(En) | - |
OMIM 2 | - |
Disease Name 3(Ja) | - |
ICD Code 3 | - |
Disease Name 3(En) | - |
OMIM 3 | - |
Age | - |
Age Range | Fetus |
Sex | Male |
Race(En) | - |
Race(Ja) | - |
Genetic Diagnosis | -- |
Not Detected | No |
Description(En) | Reprogramming of human ES-cell-derived fetal fibroblasts(dH1f). dH1f fibroblasts were subcloned from fibroblasts produced by directed differentiation of the H1-OGN human ESC line. iPSCs were derived using modified mRNAs coding reprogramming factors (KMOS) with molar concentrations in the ratio 1:1:3:1, in an atmosphere with 5% oxygenLow-oxygen culture conditions. |
Description(Ja) | ヒトES細胞由来線維芽細胞(dH1f)に、低酸素(5%)条件下で、合成mRNAを導入させて作製したiPS細胞 |
Cell Morphology | human ES-like |
Grade | Research Grade |
Vector | Other |
Transgene | KLF4 ,c-MYC,OCT4,SOX2 |
Adhesiveness | - |
Feeder | Yes |
Feeder Cell | γ -irradiated human neonatal fibroblast feeders (GlobalStem) |
Medium | DMEM/F12 , 20% KOSR ,10ng/ml FGF, nonessential amino acids (Invitrogen), 0.1 mM β-ME (Sigma), 1 mM L-glutamine (Invitrogen), plus antibiotics |
Genome Editing | - |
CO2 | - |
Mycoplasma | - |
Detection of Contaminants Mycoplasma | - |
Pluripotent Markers | Yes |
Pluripotent Markers Assay | Immunohistochemistry |
in vitro Differentiation | Yes |
in vitro Differentiation Assay | embryoid body formation |
in vivo Differentiation | - |
in vivo Differentiation Assay | - |
Other 1 Assay | - |
Other 1 Assay Method | - |
Other 2 Assay | - |
Other 2 Assay Method | - |
Other 3 Assay | - |
Other 3 Assay Method | - |
Karyotype | - |
Karyotype Assay | - |
Remaining Vector Detection | - |
Remaining Vector Detection Assay | - |
STR | - |
HLA | - |
Stem Cell Transcriptome analysis | - |
Stem Cell Transcriptome analysis Assay | - |
Author Name(En) | - |
Author Name(Ja) | - |
Author Organization(En) | - |
Author Organization(Ja) | - |
Author Contact Email | - |
PI Organization(En) | Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston |
PI Organization(Ja) | Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston |
PI Name(En) | Derrick J. Rossi |
PI Name(Ja) | Derrick J. Rossi |
PI Contact Email | rossi[at]idi[dot]harvard[dot]edu |
Availability | Information Only |
Provider Organization(En) | Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston |
Provider Organization(Ja) | Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston |
Provider Email | rossi[at]idi[dot]harvard[dot]edu |
Provider URL | - |
Ethical Statement(En) | - |
Ethical Statement(Ja) | - |
Terms of Use(En) | - |
Terms of Use(Ja) | - |
PubMed ID | 20888316 -- 21368825 |
DOI | 10.1016/j.stem.2010.08.012 -- 10.1038/nature09805 |
Title | Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. -- Somatic coding mutations in human induced pluripotent stem cells. |
Authors | Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ -- Gore A, Li Z, Fung HL, Young JE, Agarwal S, Antosiewicz-Bourget J, Canto I, Giorgetti A, Israel MA, Kiskinis E, Lee JH, Loh YH, Manos PD, Montserrat N, Panopoulos AD, Ruiz S, Wilbert ML, Yu J, Kirkness EF, Izpisua Belmonte JC, Rossi DJ, Thomson JA, Eggan K, Daley GQ, Goldstein LS, Zhang K |
Journal | Cell Stem Cell -- Nature |
Year | 2010 -- 2011 |
Volume | 7 -- 471 |
Issue | 5 -- 7336 |
Pages | 618-30 -- 63-7 |
URL | http://www.ncbi.nlm.nih.gov/pubmed/20888316 -- http://www.ncbi.nlm.nih.gov/pubmed/21368825 |
Free input | -- |
Note | - |