SKIP000673
SKIP ID SKIP000673
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) -
Cell Tissue(Ja) -
Cell Origin Normal
Cell Name 1(En) dH1f-RiPS-1.14
Cell Name 1(Ja) dH1f-RiPS-1.14
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range Fetus
Sex Male
Race(En) -
Race(Ja) -
Genetic Diagnosis --
Not Detected No
Description(En) Reprogramming of human ES-cell-derived fetal fibroblasts(dH1f).
dH1f fibroblasts were subcloned from fibroblasts produced by directed differentiation of the H1-OGN human ESC line.
iPSCs were derived using modified mRNAs coding reprogramming factors (KMOS) with molar concentrations in the ratio 1:1:3:1, in an atmosphere with 5% oxygenLow-oxygen culture conditions.
Description(Ja) ヒトES細胞由来線維芽細胞(dH1f)に、低酸素(5%)条件下で、合成mRNAを導入させて作製したiPS細胞
Cell Morphology human ES-like
Grade Research Grade
Vector Other
Transgene KLF4 ,c-MYC,OCT4,SOX2
Adhesiveness -
Feeder Yes
Feeder Cell γ -irradiated human neonatal fibroblast feeders (GlobalStem)
Medium DMEM/F12 , 20% KOSR ,10ng/ml FGF, nonessential amino acids (Invitrogen), 0.1 mM β-ME (Sigma), 1 mM L-glutamine (Invitrogen), plus antibiotics
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay Immunohistochemistry
in vitro Differentiation Yes
in vitro Differentiation Assay embryoid body formation
in vivo Differentiation -
in vivo Differentiation Assay -
Other 1 Assay -
Other 1 Assay Method -
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype -
Karyotype Assay -
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) -
Author Name(Ja) -
Author Organization(En) -
Author Organization(Ja) -
Author Contact Email -
PI Organization(En) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
PI Organization(Ja) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
PI Name(En) Derrick J. Rossi
PI Name(Ja) Derrick J. Rossi
PI Contact Email rossi[at]idi[dot]harvard[dot]edu
Availability Information Only
Provider Organization(En) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
Provider Organization(Ja) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
Provider Email rossi[at]idi[dot]harvard[dot]edu
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 20888316
--
21368825
DOI 10.1016/j.stem.2010.08.012
--
10.1038/nature09805
Title Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA.
--
Somatic coding mutations in human induced pluripotent stem cells.
Authors Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ
--
Gore A, Li Z, Fung HL, Young JE, Agarwal S, Antosiewicz-Bourget J, Canto I, Giorgetti A, Israel MA, Kiskinis E, Lee JH, Loh YH, Manos PD, Montserrat N, Panopoulos AD, Ruiz S, Wilbert ML, Yu J, Kirkness EF, Izpisua Belmonte JC, Rossi DJ, Thomson JA, Eggan K, Daley GQ, Goldstein LS, Zhang K
Journal Cell Stem Cell
--
Nature
Year 2010
--
2011
Volume 7
--
471
Issue 5
--
7336
Pages 618-30
--
63-7
URL http://www.ncbi.nlm.nih.gov/pubmed/20888316
--
http://www.ncbi.nlm.nih.gov/pubmed/21368825
Free input
--
Note -