SKIP000680
SKIP ID SKIP000680
Organism(En) -
Organism(Ja) -
Cell Type(En) -
Cell Type(Ja) -
Cell Tissue(En) Lung
Cell Tissue(Ja) Lung
Cell Origin Normal
Cell Name 1(En) MRC5-RiPS-1.11
Cell Name 1(Ja) MRC5-RiPS-1.11
Cell Name 2(En) -
Cell Name 2(Ja) -
Disease Name 1(Ja) -
ICD Code 1 -
Disease Name 1(En) -
OMIM1 -
Disease Name 2(Ja) -
ICD Code 2 -
Disease Name 2(En) -
OMIM 2 -
Disease Name 3(Ja) -
ICD Code 3 -
Disease Name 3(En) -
OMIM 3 -
Age -
Age Range Fetus
Sex Male
Race(En) -
Race(Ja) -
Genetic Diagnosis --
Not Detected No
Description(En) hiPS-cells derived from human fetal lung fibroblast cells (MRC-5 Line).
They were derived using modified mRNAs coding reprogramming factors OCT4, SOX2, KLF4, c-MYC and LIN28 (OSKML) with molar concentrations in the ratio 3:1:1:1:1.
Description(Ja) 胎児肺線維芽細胞由来iPS細胞。
低酸素下でKlf4, c-Myc, Oct4, Sox2とLIN28を3:1:1:1:1の割合でコードした合成RNAを、線維芽細胞株に20日間毎日添加し、遺伝子を細胞へ導入した。
Cell Morphology human ES-like
Grade Research Grade
Vector Other
Transgene OCT4 , SOX2 , KLF4 , c-MYC and LIN28 (OSKML)
Adhesiveness -
Feeder Yes
Feeder Cell γ -irradiated human neonatal fibroblast feeders (GlobalStem)
Medium DMEM/F12 + 20% KOSR + 5ng/ml FGF+(-)valproic acid (VPA)+a histone deacetylase inhibitor
Genome Editing -
CO2 -
Mycoplasma -
Detection of Contaminants Mycoplasma -
Pluripotent Markers Yes
Pluripotent Markers Assay Immunostaining
in vitro Differentiation Yes
in vitro Differentiation Assay embryoid body formation
in vivo Differentiation -
in vivo Differentiation Assay -
Other 1 Assay Yes
Other 1 Assay Method qRT-PCR
Other 2 Assay -
Other 2 Assay Method -
Other 3 Assay -
Other 3 Assay Method -
Karyotype Yes
Karyotype Assay -
Remaining Vector Detection -
Remaining Vector Detection Assay -
STR -
HLA -
Stem Cell Transcriptome analysis -
Stem Cell Transcriptome analysis Assay -
Author Name(En) -
Author Name(Ja) -
Author Organization(En) -
Author Organization(Ja) -
Author Contact Email -
PI Organization(En) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
PI Organization(Ja) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
PI Name(En) Derrick J. Rossi
PI Name(Ja) Derrick J. Rossi
PI Contact Email -
Availability Information Only
Provider Organization(En) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
Provider Organization(Ja) Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston
Provider Email rossi[at]idi[dot]harvard[dot]edu
Provider URL -
Ethical Statement(En) -
Ethical Statement(Ja) -
Terms of Use(En) -
Terms of Use(Ja) -
PubMed ID 20888316
DOI 10.1016/j.stem.2010.08.012
Title Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA.
Authors Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ
Journal Cell Stem Cell
Year 2010
Volume 7
Issue 5
Pages 618-30
URL http://www.ncbi.nlm.nih.gov/pubmed/20888316
Free input -
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