| SKIP001096 |
| SKIP ID |
SKIP001096 |
| Organism(En) |
- |
| Organism(Ja) |
- |
| Cell Type(En) |
- |
| Cell Type(Ja) |
- |
| Cell Tissue(En) |
- |
| Cell Tissue(Ja) |
- |
| Cell Origin |
Normal |
| Cell Name 1(En) |
WT-1-#2 |
| Cell Name 1(Ja) |
WT-1-#2 |
| Cell Name 2(En) |
- |
| Cell Name 2(Ja) |
- |
| Disease Name 1(Ja) |
- |
| ICD Code 1 |
- |
| Disease Name 1(En) |
- |
| OMIM1 |
- |
| Disease Name 2(Ja) |
- |
| ICD Code 2 |
- |
| Disease Name 2(En) |
- |
| OMIM 2 |
- |
| Disease Name 3(Ja) |
- |
| ICD Code 3 |
- |
| Disease Name 3(En) |
- |
| OMIM 3 |
- |
| Age |
- |
| Age Range |
0-9 |
| Sex |
Male |
| Race(En) |
- |
| Race(Ja) |
- |
| Genetic Diagnosis |
-- |
| Not Detected |
No |
| Description(En) |
Nomal human iPS cell line derived from a neonate.(1439 KURABO)
|
| Description(Ja) |
新生児皮膚繊維芽細胞(1439 KURABO)由来iPS細胞 |
| Cell Morphology |
human ES-like |
| Grade |
Research Grade |
| Vector |
Other |
| Transgene |
Mixture Y4: OCT3/4 , SOX2 , KLF4 , L-MYC , LIN28 , and p53 shRNA |
| Adhesiveness |
- |
| Feeder |
Yes |
| Feeder Cell |
Mitomycin-C-treated mouse embryonic fibroblasts |
| Medium |
MEM/F12 (Sigma) with 20% Knockout Serum Replacement (Invitrogen), 2 m m l -glutamine, 100 μ m non-essential amino acids, 100 μ m β -mercaptoethanol, 4 ng/ml bFGF (Wako), 50 U/ml penicillin and 50 μg/ml streptomycin |
| Genome Editing |
- |
| CO2 |
- |
| Mycoplasma |
- |
| Detection of Contaminants Mycoplasma |
- |
| Pluripotent Markers |
Yes |
| Pluripotent Markers Assay |
Immunocytochemical analysis |
| in vitro Differentiation |
Yes |
| in vitro Differentiation Assay |
- |
| in vivo Differentiation |
Yes |
| in vivo Differentiation Assay |
Teratoma formation |
| Other 1 Assay |
- |
| Other 1 Assay Method |
- |
| Other 2 Assay |
- |
| Other 2 Assay Method |
- |
| Other 3 Assay |
- |
| Other 3 Assay Method |
- |
| Karyotype |
No |
| Karyotype Assay |
- |
| Remaining Vector Detection |
- |
| Remaining Vector Detection Assay |
- |
| STR |
- |
| HLA |
- |
| Stem Cell Transcriptome analysis |
- |
| Stem Cell Transcriptome analysis Assay |
- |
| Author Name(En) |
Minoru Okada |
| Author Name(Ja) |
岡田 稔 |
| Author Organization(En) |
Center for iPS Cell Research and Application, Kyoto University |
| Author Organization(Ja) |
京都大学iPS細胞研究所 |
| Author Contact Email |
ntsumaki[at]cira[dot]kyoto-u[dot]ac[dot]jp |
| PI Organization(En) |
- |
| PI Organization(Ja) |
- |
| PI Name(En) |
- |
| PI Name(Ja) |
- |
| PI Contact Email |
- |
| Availability |
Information Only |
| Provider Organization(En) |
Center for iPS Cell Research and Application,Kyoto University |
| Provider Organization(Ja) |
京都大学iPS細胞研究所 CiRA |
| Provider Email |
ntsumaki[at]cira[dot]kyoto-u[dot]ac[dot]jp |
| Provider URL |
https://www.cira.kyoto-u.ac.jp/e/index.html |
| Ethical Statement(En) |
- |
| Ethical Statement(Ja) |
- |
| Terms of Use(En) |
- |
| Terms of Use(Ja) |
- |
| PubMed ID |
25187577 |
| DOI |
10.1093/hmg/ddu444 |
| Title |
Modeling type II collagenopathy skeletal dysplasia by directed conversion and induced pluripotent stem cells. |
| Authors |
Okada M, Ikegawa S, Morioka M, Yamashita A, Saito A, Sawai H, Murotsuki J, Ohashi H, Okamoto T, Nishimura G, Imaizumi K, Tsumaki N |
| Journal |
Hum Mol Genet |
| Year |
2014 |
| Volume |
24 |
| Issue |
2 |
| Pages |
299-313 |
| URL |
http://www.ncbi.nlm.nih.gov/pubmed/25187577 |
| Free input |
- |
| Note |
- |